In Vivo Dual-Modality Fluorescence and Magnetic Resonance Imaging-Guided Lymph Node Mapping with Good Biocompatibility Manganese Oxide Nanoparticles

Multifunctional manganese oxide nanoparticles (NPs) with impressive enhanced T1 contrast ability show great promise in biomedical diagnosis. Herein, we developed a dual-modality imaging agent system based on polyethylene glycol (PEG)-coated manganese oxide NPs conjugated with organic dye (Cy7.5), which functions as a fluorescence imaging (FI) agent as well as a magnetic resonance imaging (MRI) imaging agent. The formed [email protected] NPs with the size of ~10 nm exhibit good colloidal stability in different physiological media. Serial FI and MRI studies that non-invasively assessed the bio-distribution pattern and the feasibility for in vivo dual-modality imaging-guided lymph node mapping have been investigated. In addition, histological and biochemical analyses exhibited low toxicity even at a dose of 20 mg/kg in vivo. Since [email protected] NPs exhibited desirable properties as imaging agents and good biocompatibility, this work offers a robust, safe, and accurate diagnostic platform based on manganese oxide NPs for tumor metastasis diagnosis

The Clinicopathologic Importance of Serum Lactic Dehydrogenase in Patients with Gastric Cancer

Background. To explore possible correlation between serum lactate dehydrogenase (SLDH) levels and gastric cancer. Materials and Methods. We retrospectively reviewed 365 patients with gastric cancer. The correlation of SLDH levels with clinicopathologic features and survival rate was studied. Results. SLDH levels were closely associated with the pathological (p) T stage (P=0.011), metastasis (P=0.012), pTNM stage (P=0.001), and recurrence (P=0.012). Moreover, we found a significant SLDH level difference among Borrmann type (P=0.027), pT stage (P=0.004), lymph node metastasis (P=0.027), metastasis (P<0.001), pTNM stage (P=0.006), and recurrence (P=0.002). In addition, we detected a significant SLDH level difference between alive and dead subgroups (P=0.001). In addition, both univariate analysis and multivariate analysis showed that high SLDH levels were independent prognostic factor. For the subgroup with normal LDH (median point of 157.0 U/L), we detected that the subset with SLDH levels ≥157 U/L (158–245 U/L) showed poorer OS (P=0.005) and DFS (P=0.01) than that of ≤157 subgroup. Conclusions. Our results suggest that high SLDH level could be an independent poor prognostic biomarker. Gastric cancer patients with relative high SLDH level (158–245 U/L) were prone to develop a shorter OS and DFS

High-temperature PTT/CDT coordination nanoplatform realizing exacerbated hypoxia for enhancing hypoxia-activated chemotherapy to overcome tumor drug resistance

Abstract Background Hypoxia-activated prodrugs present new opportunities for safe and effective tumor drug resistance therapy due to their high selectivity for hypoxic cells. However, the uneven distribution of oxygen in solid tumor and insufficient hypoxia in the tumor microenvironment greatly limit its therapeutic efficacy. Results In this paper, a novel AQ4N-Mn(II)@PDA coordination nanoplatform was designed and functionalized with GMBP1 to target drug-resistant tumor cells. Its excellent photothermal conversion efficiency could achieve local high-temperature photothermal therapy in tumors, which could not only effectively exacerbate tumor hypoxia and thus improve the efficacy of hypoxia-activated chemotherapy of AQ4N but also significantly accelerate Mn2+-mediated Fenton-like activity to enhance chemodynamic therapy. Moreover, real-time monitoring of blood oxygen saturation through photoacoustic imaging could reflect the hypoxic status of tumors during treatment. Furthermore, synergistic treatment effectively inhibited tumor growth and improved the survival rate of mice bearing orthotopic drug-resistant tumors. Conclusions This study not only provided a new idea for PTT combined with hypoxia-activated chemotherapy and CDT for drug-resistant tumors but also explored a vital theory for real-time monitoring of hypoxia during treatment

Facile Synthesis of FeOOH Quantum Dots Modified ZnO Nanorods Films via a Metal-Solating Process

In this study, we referenced the formation principle of rust in nature and the FeOOH quantum dots (QDs) were prepared using a metal-solating process. The FeOOH QDs exhibited an average diameter of 3.5 nm with well crystallinity. Furthermore, the as-prepared FeOOH QDs were deposited on ZnO nanorods film as a co-catalyst for water oxidation. The crystal phase, microstructures, and optical properties of the synthesized films were established through X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, and ultraviolet–visible absorption spectroscopy (UV-vis). Applied as a photoanode for solar water splitting, the FeOOH QDs/ZnO nanorods film exhibited a photocurrent density of 0.44 mA/cm² at 1.23 V vs RHE, which is 2.1 times higher than that of pure ZnO film. After the loading of FeOOH QDs, the ZnO photoanode showed higher surface charge injection efficiency (by a factor of ∼2) and better long-term stability. The analysis of electrochemical measurements displayed that, as a co-catalyst of the oxygen evolution reaction, FeOOH QDs resulted in a noticeable cathodic shift of photocurrent onset potential for water oxidation and a remarkable improvement of surface charge injection efficiency. In addition, the metal-solating method can be applied to preparing the other metal oxides QDs, such as WO₃ and ZnO QDs