Apparatus and method for deforming a workpiece

An apparatus and method is provided for controlling the temperature of dies and workpiece throughout a deformation process. Dies are capable of receiving external current to resistively heat the workpiece and dies during the deformation operation, the external current being modulated by feedback temperature readings taken from the workpiece. External current may be provided from a homopolar generator capable of producing pulsed dc current at controllable intervals and magnitudes simultaneously through the dies and workpiece.Board of Regents, University of Texas Syste

Method and construction for control of current distribution in railgun armatures

An electromagnetic railgun having a layer of low conductivity material along the rails to interface with the armature as the armature is propelled along the rails. The low conductivity material has been found to permit rapid current penetration between the layer and armature, thereby inhibiting undersirable high current density formation along the trailing edge of the armature. Advantageously, inhibiting the high current density formation permits use of a solid armature at velocities exceeding on kilometer per second. Preferably, the layer is composed of graphite or a graphite/copper mixture. A layer of copper or other high conductivity material is preferably laminated to the low conductivity graphite layer to reduce the overall railgun circuit resistance. In the preferred embodiment, the layer of low conductivity material increases in thickness from the breech to the muzzle to decrease railgun circuit resistance.Board of Regents, University of Texas Syste

Method and construction for control of current distribution in railgun armatures

An electromagnetic railgun having a layer of low conductivity material along the rails to interface with the armature as the armature is propelled along the rails. The low conductivity material has been found to permit rapid current penetration between the layer and armature, thereby inhibiting undesirable high current density formation along the trailing edge of the armature. Advantageously, inhibiting the high current density formation permits use of a solid armature at velocities exceeding one kilometer per second. Preferably, the layer is composed of graphite or a graphite/copper mixture. A layer of copper or other high conductivity material is preferably laminated to the low conductivity graphite layer to reduce the overall railgun circuit resistance. In the preferred embodiment, the layer of low conductivity material increases in thickness from the breech to the muzzle to decrease railgun circuit resistance.Board of Regents, University of Texas Syste

Staged opening switch

" A staged switch for conducting a large electrical current for an indefinite time period and then rapidly switching the current into a load circuit. The switch comprises a first stage which can conduct a large electrical current for an indefinite time period without damage to the stage and a second stage which is a fast-opening element. The first stage includes primary and secondary contacts wherein the primary contacts conduct a charging current and the secondary contacts divert the current into the fast-opening second stage. The secondary contacts ""make"" before the primary contacts ""break"", reducing the damage to the contacts which might otherwise be experienced. The second stage is a low-inductance element which reduces the level of energy absorbed by the first-stage contacts, minimizing damage to the contacts. The second-stage element may be constructed in a cartridge and may be loaded into the switch using an autoloader device. "Board of Regents, University of Texas Syste

Do SASS5 scores vary with season in the South African highveld? A case study on the Skeerpoort River, North West province, South Africa

Macroinvertebrate assemblages can vary with season as a result of changing environmental conditions and phenology of individual species. The objective of this study was to determine whether results from the South African Scoring System version 5 (SASS5) are affected by season. The standard SASS5 protocol was used to sample three sites on the Skeerpoort River, South Africa, in autumn, winter and spring 2013, and summer 2014. At each site, stream morphology was characterised and physicochemical variables were measured. Two-way analysis of variance (ANOVA) was used to compare SASS indices in relation to site and season, and a one-way ANOVA was used to test the effect of season using both current and historical data. Macroinvertebrate assemblage similarity of sites was determined using nonmetric multidimensional scaling ordination, followed by analysis of similarity. Whilst macroinvertebrate assemblages differed between seasons and sites, there was no seasonal variation in the values of SASS indices, but they did differ between sites. SASS5 appears to be robust to seasonal variation in macroinvertebrate assemblage and seems suitable for use throughout the year in a perennial river draining the South African highveld region.University of Pretoria through a Research Development Programme.http://www.tandfonline.com/loi/taas202015-12-30hb201

Cold Chain and Virus‐Free Chloroplast‐Made Booster Vaccine to Confer Immunity Against Different Poliovirus Serotypes

The WHO recommends complete withdrawal of oral polio vaccine (OPV) type 2 by April 2016 globally and replacing with at least one dose of inactivated poliovirus vaccine (IPV). However, high‐cost, limited supply of IPV, persistent circulating vaccine‐derived polioviruses transmission and need for subsequent boosters remain unresolved. To meet this critical need, a novel strategy of a low‐cost cold chain‐free plant‐made viral protein 1 (VP1) subunit oral booster vaccine after single IPV dose is reported. Codon optimization of the VP1 gene enhanced expression by 50‐fold in chloroplasts. Oral boosting of VP1 expressed in plant cells with plant‐derived adjuvants after single priming with IPV significantly increased VP1‐IgG1 and VP1‐IgA titres when compared to lower IgG1 or negligible IgA titres with IPV injections. IgA plays a pivotal role in polio eradication because of its transmission through contaminated water or sewer systems. Neutralizing antibody titres (~3.17–10.17 log2 titre) and seropositivity (70–90%) against all three poliovirus Sabin serotypes were observed with two doses of IPV and plant‐cell oral boosters but single dose of IPV resulted in poor neutralization. Lyophilized plant cells expressing VP1 stored at ambient temperature maintained efficacy and preserved antigen folding/assembly indefinitely, thereby eliminating cold chain currently required for all vaccines. Replacement of OPV with this booster vaccine and the next steps in clinical translation of FDA‐approved antigens and adjuvants are discussed

Enhanced Immunogenicity of Stabilized Trimeric Soluble Influenza Hemagglutinin

The recent swine-origin H1N1 pandemic illustrates the need to develop improved procedures for rapid production of influenza vaccines. One alternative to the current egg-based manufacture of influenza vaccine is to produce a hemagglutinin (HA) subunit vaccine using a recombinant expression system with the potential for high protein yields, ease of cloning new antigenic variants, and an established safety record in humans.We generated a soluble HA (sHA), derived from the H3N2 virus A/Aichi/2/68, modified at the C-terminus with a GCN4pII trimerization repeat to stabilize the native trimeric structure of HA. When expressed in the baculovirus system, the modified sHA formed native trimers. In contrast, the unmodified sHA was found to present epitopes recognized by a low-pH conformation specific monoclonal antibody. We found that mice primed and boosted with 3 microg of trimeric sHA in the absence of adjuvants had significantly higher IgG and HAI titers than mice that received the unmodified sHA. This correlated with an increased survival and reduced body weight loss following lethal challenge with mouse-adapted A/Aichi/2/68 virus. In addition, mice receiving a single vaccination of the trimeric sHA in the absence of adjuvants had improved survival and body weight loss compared to mice vaccinated with the unmodified sHA.Our data indicate that the recombinant trimeric sHA presents native trimeric epitopes while the unmodified sHA presents epitopes not exposed in the native HA molecule. The epitopes presented in the unmodified sHA constitute a "silent face" which may skew the antibody response to epitopes not accessible in live virus at neutral pH. The results demonstrate that the trimeric sHA is a more effective influenza vaccine candidate and emphasize the importance of structure-based antigen design in improving recombinant HA vaccines

High performance electromagnetic railgun launcher

A railgun operates at high pressure (up to 350 MPa) without structural damage and is readily disassembled for inspection, maintenance and component testing. A rail assembly is pressed into a hoop-wound epoxy fiberglass containment tube and clamped within a steel compression frame. The geometry of the rail assembly permits rail movement without insulator intrusion and achieves bore sealing during rail movement at maximum pressure. The rail assembly also has replaceable insulator inserts which are isolated from rail re-bound shock. Fused quartz insulator inserts provide the best results. A flash tube is provided at the gun muzzle to suppress precursor discharge and commutate precursor current back to the armature. To realize increased velocity without sacrificing in-bore projectile stability, a cut-corner projectile is used having a L/D ratio as small as 0.65 which reduces the mass by about 11%.Board of Regents, University of Texas Syste

Selection on female reproductive schedules in the marula fly, ceratitis cosyra (Diptera : Tephritidae) affects dietary optima for female reproductive traits but not lifespan

DATA AVAILABILITY STATEMENT : The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.INTRODUCTION : A changing environment can select on life-history traits and tradeoffs in a myriad of ways. For example, global warming may shift phenology and thus the availability of host-plants. This may alter selection on survival and fertility schedules in herbivorous insects. If selection on life-histories changes, this may in turn select for altered nutrient intake, because the blend of nutrients organisms consume helps determine the expression of life-history traits. However, we lack empirical work testing whether shifts in the timing of oviposition alter nutrient intake and life-history strategies. METHODS : We tested in the marula fruit fly, Ceratitis cosyra, how upward-selection on the age of female oviposition, in comparison with laboratory adapted control flies, affects the sex-specific relationship between protein and carbohydrate intake and life-history traits including lifespan, female lifetime egg production and daily egg production. We then determined the macronutrient ratio consumed when flies from each selection line and sex were allowed to self-regulate their intake. RESULTS : Lifespan, lifetime egg production and daily egg production were optimised at similar protein to carbohydrate (P:C) ratios in flies from both selection lines. Likewise, females and males of both lines actively defended similar nutrient intake ratios (control =1:3.6 P:C; upward-selected = 1:3.2 P:C). DISCUSSION : Our results are comparable to those in non-selected C. cosyra, where the optima for each trait and the self-selected protein to carbohydrate ratio observed were nearly identical. The nutrient blend that needs to be ingested for optimal expression of a given trait appeared to be well conserved across laboratory adapted and experimentally selected populations. These results suggest that in C. cosyra, nutritional requirements do not respond to a temporal change in oviposition substrate availability.A National Research Foundation Competitive Programme for Rated Researchers grant and the Australian Research Council.https://www.frontiersin.org/journals/insect-scienceam2024Zoology and EntomologySDG-15:Life on lan

Immunity and field efficacy of type 2-containing polio vaccines after cessation of trivalent oral polio vaccine: A population-based serological study in Pakistan

Background: In Pakistan and other countries using oral polio vaccine (OPV), immunity to type 2 poliovirus is now maintained by a single dose of inactivated polio vaccine (IPV) in routine immunization, supplemented in outbreak settings by monovalent OPV type 2 (mOPV2) and IPV. While well-studied in clinical trials, population protection against poliovirus type 2 achieved in routine and outbreak settings is generally unknown.Methods: We conducted two phases of a population-based serological survey of 7940 children aged 6-11 months old, between November 2016 and October 2017 from 13 polio high-risk locations in Pakistan.Results: Type 2 seroprevalence was 50% among children born after trivalent OPV (tOPV) withdrawal (April 2016), with heterogeneity across survey areas. Supplementary immunization activities (SIAs) with mOPV2 followed by IPV improved population immunity, varying from 89% in Pishin to 64% in Killa Abdullah, with little observed marginal benefit of subsequent campaigns. In the other high-risk districts surveyed, a single SIA with IPV was conducted and appeared to improve immunity to 57% in Karachi to 84% in Khyber.Conclusions: Our study documents declining population immunity following trivalent OPV withdrawal in Pakistan, and wide heterogeneity in the population impact of supplementary immunization campaigns. Differences between areas, attributable to vaccination campaign coverage, were far more important for type 2 humoral immunity than the number of vaccination campaigns or vaccines used. This emphasizes the importance of immunization campaign coverage for type 2 outbreak response in the final stages of polio eradication. Given the declining type 2 immunity in new birth cohorts it is also recommended that 2 or more doses of IPV should be introduced in the routine immunization program of Pakistan