888 research outputs found
Rat Monoclonal Antibodies Specific for LST1 Proteins
The LST1 gene is located in the human MHC class III region and encodes transmembrane and soluble isoforms that have been suggested to play a role in the regulation of the immune response and are associated with inflammatory diseases such as rheumatoid arthritis. Here we describe the generation and characterization of the first monoclonal antibodies against LST1. Two hybridoma lines secreting monoclonal antibodies designated 7E2 and 8D12 were established. The 7E2 antibody detects recombinant and endogenous LST1 by Western blot analysis while 8D12 reacts with recombinant and endogenous LST1 in immunoprecipitation and flow cytometry procedures. The newly established antibodies were used to survey LST1 protein expression in human cell lines, which was found to be tightly regulated, allowing the expression of transmembrane isoforms but suppressing soluble isoforms
TUMOR NECROSIS FACTOR BETA (TNF-P) INDUCES BINDING OF THE NF-KB TRANSCRIPTION FACTOR TO A HIGH-AFFINITY KB ELEMENT IN THE TNF-BETA PROMOTER
Complete sequence of HLA-B27 cDNA identified through the characterization of structural markers unique to the HLA-A, -B, and -C allelic series
Antigen HLA-B27 is a high-risk genetic factor
with respect to a group of rheumatoid disorders, especially
ankylosing spondylitis. A cDNA library was constructed from
an autozygous B-cell line expressing HLA-B27, HLA-Cw1, and
the previously cloned HLA-A2 antigen. Clones detected with an
HLA probe' were isolated and sorted into homology groups by
differential hybridization and restriction maps. Nucleotide
sequencing allowed the unambiguous assignment of cDNAs to
HL4-A, -B, and -C loci. The HLA-B27 mRNA has the
structural features and the codon variability typical of an HLA
class I transcript but it specifies two uncommon amino acid
replacements: a cysteine in position 67 and a serine in position
131. The latter substitution may have functional consequences,
because it occurs in a conserved region and at a position
invariably occupied by a species-specific arginine in humans
and lysine in mice. The availability of the complete sequence of
HLA-B27 and of the partial sequence of HLA-Cw1 allows the
recognition of locus-specific sequence markers, particularly,
but not exclusively, in the transmembrane and cytoplasmic
domains
Identification of murine H-2Db histocompatibility antigens in cells transfected with cloned H-2 genes
Presence of an expressed 13-tubulin gene (TUBB) in the HLA class I region may provide the genetic basis for HLA-linked microtubule dysfunction
An expressed beta-tubulin gene (TUBB) has previously
been localized to chromosome region 6pter-p21
in man. By using a panel of deletion mutant cell lines and
radiation-reduced hybrids containing fragments of chromosome
6, the TUBB locus could be mapped to the HLA
class I region at 6p21.3. A long range restriction map including
TUBB and several HLA class I genes was then
generated by rotating field gel electrophoresis. The results
show that TUBB maps to a segment 170-370 kb telomeric
of HLA-C. This location suggests that a mutation at the
TUBB locus could be the cause for certain forms of HLAlinked
microtubule dysfunction, including immotile cilia
syndrome
Localization of the genes for tumor necrosis factor and lymphotoxin between the HLA classI and III regions by field inversion gel electrophoresis
To clarify the position of the TNFA and TNFB genes on the HLA map, we have assigned TNFA to large DNA restriction fragments separated by field inversion gel electrophoresis, which hybridize with either class III- or class I-specific probes as well. These results prove that the TNFA locus is localized between the HLA class III region and the HLA-B locus
Human interleukin-1 receptor antagonist is expressed in liver
AbstractUsing PCR and Northern blot analysis, an IL-1 receptor antagonist specific transcript was amplified from HepG2- and liver mRNA, cDNA clones coding for IL-1 receptor antagonist were isolated from a liver cDNA library and sequence comparison revealed complete identity with the secreted, monocytic form of IL-1 receptor antagonist
Impaired Intracellular Transport and Cell Surface Expression of Nonpolymorphic HLA-E
The assembly of the classical, polymorphic major histocompatibility complex class I molecules
in the endoplasmic reticulum requires the presence of peptide ligands and ~2-microglobulin
(~2m). Formation of this trimolecular complex is a prerequisite for e~cient transport to the
cell surface, where presented peptides are scanned by T lymphocytes. The function of the other
class I molecules is in dispute. The human, nonclassical class I gene, HLA-E, was found to be
ubiquitously transcribed, whereas cell surface expression was dif~cult to detect upon transfection.
Pulse chase experiments revealed that the HLA-E heavy chain in transfectants, obtained with
the murine myeloma cell line P3X63-Ag8.653 (X63), displays a significant reduction in
oligosaccharide maturation and intracellular transport compared with HLA-B27 in corresponding
transfectants. The accordingly low HLA-E cell surface expression could be significantly enhanced
by either reducing the culture temperature or by supplementing the medium with human ~2m,
suggesting inefficient binding of endogenous peptides to HLA-E. To analyze whether HLA-E
binds peptides and to identify the corresponding ligands, fractions of acid-extracted material from
HLA-E/X63 transfectants were separated by reverse phase HPLC and were tested for their ability
to enhance HLA-E cell surface expression. Two fractions specifically increased the HLA class
I expression on the HLA-E transfectant clone
Centromerus silvicola and C. sellarius (C. similis) - clarifying comments on nomenclature
Bei Untersuchungen der Spinnenfauna im Nationalpark Bayerischer Wald fanden wir eine Centromerus-Art, die nach der Struktur des männlichen Pedipalpus zunächst keiner Spezies einwandfrei zuzuordnen war. Wir hatten die Vermutung, dal1 es sich bei den vorliegenden Tieren um C. silvicola handeln könnte, da MILLER (1958) Weibchen dieser süddosteuropäisch verbreiteten Art im benachbarten Behmerwald (Sumava, Zelnava beim Moldau-Stausee) nachgewiesen hatte, und da das Männchen von C. silvicola allem Anschein nach als noch nicht beschrieben galt. Ein Vergleich dieser Tiere mit Exemplaren aus Niederbayern (Landshut), welche nach WIEHLE (1956) als C. similis KULCZVNSKI, 1894 bestimmt worden waren, erbrachte jedoch keinerlei Unterschiede
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