An Approach for Instantaneous Ambiguity Resolution for Medium- to Long-range Multiple Reference Station Networks

Integer ambiguity resolution (AR) is a prerequisite for all high-precision (centimetre level) GPS applications that utilise multiple reference station (MRS) networks. However, due to the presence of distance-dependent GPS errors, notably atmospheric refraction, AR across the network is difficult on an epoch-by-epoch basis, especially for medium- to long-range (typically 30?130 km as used here) MRS networks. This paper presents an approach for medium- to long-range instantaneous AR for MRS networks, based on an ionosphere-weighted observation model and network geometry constraints, along with a multiple ambiguity validation test procedure. The performance of the proposed method was demonstrated through two case-study examples from Australia and Norway. Our test results show that the instantaneous AR success rate varied from 93% (131 km baseline) to 98% (35 km baseline).It is also shown that the adopted high-precision prediction models for the double-difference (DD) ionospheric delay and residual tropospheric zenith delay (RTZD) are of benefit to the high success rate of the network AR. Due to its epoch-by-epoch nature, the proposed approach is insensitive to cycle-slips, rising or setting satellites, or loss-of-lock

Mechanism involved in phagocytosis and killing of Listeria monocytogenes by Acanthamoeba polyphaga

© Springer-Verlag 2009Intra-cellular pathogen, Listeria monocytogenes, is capable of invasion and survival within mammalian cells. However, Acanthamoeba polyphaga trophozoites phagocytose and rapidly degrade Listeria cells. In order to provide more information on amoeba phagocytosis and killing mechanisms, this study used several inhibitor agents known to affect the phagocytosis and killing of bacteria by eukaryotes. Amoebae were pre-treated with mannose, cytochalasin D, wortmannin, suramin, ammonium chloride, bafilomycin A and monensin followed by co-culture with bacteria. Phagocytosis and killing of bacterial cells by amoeba trophozoites was assessed using plate counting methods and microscopy. The data presented indicates that actin polymerisation and cytoskeletal rearrangement are involved in phagocytosis of L. monocytogenes cells by A. polyphaga trophozoites. Further, both phagosomal acidification and phagosome–lysosome fusion are involved in killing and degradation of L. monocytogenes cells by A. polyphaga. However, the mannose-binding protein receptor does not play an important role in uptake of bacteria by amoeba trophozoites. In conclusion, this data reveals the similar principles of molecular mechanisms used by different types of eukaryotes in uptake and killing of bacteria.Alisha Akya, Andrew Pointon and Connor Thoma