Ekspresija tanaze iz plijesni Aspergillus oryzae u kvascu Pichia pastoris i primjena u sintezi propil galata u organskom otapalu

Gallic acid esters could be synthesized biologically by tannase in organic media, among which is propyl gallate, an antioxidant widely used as a food additive. Efficient intracellular expression of Aspergillus oryzae tannase was achieved in Pichia pastoris under the control of the AOX1 promoter, and the productivity of recombinant tannase was 960 U/L or 64 U/g dry mass. The recombinant P. pastoris was used to synthesize propyl gallate in organic solvent and the yield of propyl gallate was 53 %.Esteri galne kiseline, poput propil galata, antioksidansa koji se često koristi kao prehrambeni aditiv, mogu se biološki sintetizirati pomoću tanaze u organskom otapalu. Postignuta je uspješna intracelularna ekspresija tanaze iz plijesni Aspergillus oryzae u kvascu Pichia pastoris pod kontrolom promotora AOX1, kao i prinos rekombinantne tanaze od 960 U/L ili 64 U/g suhe tvari. Rekombinantni soj P. pastoris upotrijebljen je za sintezu propil galata u organskom otapalu, pri čemu je postignut prinos propil galata od 53 %

Guest Editorial: Nonlinear Optimization of Communication Systems

Linear programming and other classical optimization techniques have found important applications in communication systems for many decades. Recently, there has been a surge in research activities that utilize the latest developments in nonlinear optimization to tackle a much wider scope of work in the analysis and design of communication systems. These activities involve every “layer” of the protocol stack and the principles of layered network architecture itself, and have made intellectual and practical impacts significantly beyond the established frameworks of optimization of communication systems in the early 1990s. These recent results are driven by new demands in the areas of communications and networking, as well as new tools emerging from optimization theory. Such tools include the powerful theories and highly efficient computational algorithms for nonlinear convex optimization, together with global solution methods and relaxation techniques for nonconvex optimization

Announcing Molecular Biomedicine

Molecular Biomedicine is a peer-reviewed and open- access journal launched by Springer Nature in 2020, publishing the pioneer works in molecular medicine. We appreciate for the sincere support of many talented sci- entists and academic institutions worldwide that inspired us to start this journal