121 research outputs found
Mortality percentage of Sri Lankan Leopards (Pantera pardus kotiya)
leopard, mortality, sri lankan, Π»Π΅ΠΎΠΏΠ°ΡΠ΄, ΡΠΌΠ΅ΡΡΠ½ΠΎΡΡΡ, Π¨ΡΠΈ-ΠΠ°Π½ΠΊ
Anthelmintic efficacy of Albendazole and Levamisole against gastrointestinal parasites in Sri Lanakan elephants at Udawalawe, Sri Lanka
elephants, parasites, gastrointestinal parasites, antihelminthic drug, albendazole, levamisole, sri lanka, ΡΠ»ΠΎΠ½Ρ, ΠΏΠ°ΡΠ°Π·ΠΈΡΡ, Π³Π΅Π»ΡΠΌΠΈΠ½ΡΡ, ΠΆΠ΅Π»ΡΠ΄ΠΎΡΠ½ΠΎ-ΠΊΠΈΡΠ΅ΡΠ½ΡΠΉ ΡΡΠ°ΠΊΡ(ΠΆΠΊΡ), ΠΏΡΠΎΡΠΈΠ²ΠΎΠ³Π»ΠΈΡΡΠ½ΡΠ΅ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ, Π°Π»ΡΠ±Π΅Π½Π΄Π°Π·ΠΎΠ», Π»Π΅Π²Π°ΠΌΠΈΠ·ΠΎΠ», Π¨ΡΠΈ-ΠΠ°Π½ΠΊ
Fatality percentages of Sri Lankan leopards (Panthera pardus kotiya) killed in human activities in Sri Lanka
Π»Π΅ΠΎΠΏΠ°ΡΠ΄, ΡΠΌΠ΅ΡΡΠ½ΠΎΡΡΡ, ΠΏΡΠΈΡΠΈΠ½Ρ, ΡΠ°ΠΌΠΊΠΈ, ΡΠ°ΠΌΡΡ, Π΄Π΅ΡΡΠ΅Π»ΡΠ½ΠΎΡΡΡ Π»ΡΠ΄Π΅ΠΉ, ΡΡΠ΅Π΄Π° ΠΎΠ±ΠΈΡΠ°Π½ΠΈΡ, Π°Π³ΡΠΎΠΊΡΠ»ΡΡΡΡΠ°, Π¨ΡΠΈ ΠΠ°Π½ΠΊΠ°ΠΡΠΈΠ²Π΅Π΄Π΅Π½Ρ Π΄Π°Π½Π½ΡΠ΅ ΠΏΠΎ Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΡ Π·Π° ΠΆΠΈΠ·Π½ΡΡ Π΄ΠΈΠΊΠΈΡ
Π»Π΅ΠΎΠΏΠ°ΡΠ΄ΠΎΠ² Π΄Π»Ρ ΡΡΠΎΡΠ½Π΅Π½ΠΈΡ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΡΡ
ΠΏΡΠΈΡΠΈΠ½ ΠΈΡ
Π³ΠΈΠ±Π΅Π»ΠΈ. Π Π°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°Π»ΠΈΡΡ ΠΈ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈΡΡ ΡΠ»ΡΡΠ°ΠΈ Π³ΠΈΠ±Π΅Π»ΠΈ Π»Π΅ΠΎΠΏΠ°ΡΠ΄ΠΎΠ² Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 2010-2016 Π³ΠΎΠ΄ΠΎΠ², ΠΏΠΎΠΊΠ°Π·ΡΠ²Π°ΡΡΠΈΠ΅, ΡΡΠΎ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΉ ΠΈ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΠ°ΡΡΠΎΠΉ ΠΏΡΠΈΡΠΈΠ½ΠΎΠΉ Π³ΠΈΠ±Π΅Π»ΠΈ Π»Π΅ΠΎΠΏΠ°ΡΠ΄ΠΎΠ² ΡΠ²ΠΈΠ»ΡΡ ΡΠ΅Π»ΠΎΠ²Π΅ΡΠ΅ΡΠΊΠΈΠΉ ΡΠ°ΠΊΡΠΎΡ. Π‘ΡΠ΅Π΄ΠΈ Π²ΡΠ΅Ρ
ΠΏΠΎΠ»ΠΎΠ²ΠΎΠ·ΡΠ°ΡΡΠ½ΡΡ
Π³ΡΡΠΏΠΏ Π½Π°Π±Π»ΡΠ΄Π°Π»Π°ΡΡ ΡΡΠ° ΠΆΠ΅ ΡΠ΅Π½Π΄Π΅Π½ΡΠΈΡ, ΡΠΎ Π΅ΡΡΡ ΡΠ΅Π»ΠΎΠ²Π΅ΡΠ΅ΡΠΊΠΈΠΉ ΡΠ°ΠΊΡΠΎΡ ΡΠ°ΠΊΠΆΠ΅ ΡΠ²Π»ΡΠ»ΡΡ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΉ ΠΏΡΠΈΡΠΈΠ½ΠΎΠΉ Π³ΠΈΠ±Π΅Π»ΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
. ΠΡΠΎΡ ΠΏΡΠΎΡΠ΅Π½Ρ Π³ΠΈΠ±Π΅Π»ΠΈ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΡΠ½ΠΈΠΆΠ΅Π½ Π·Π° ΡΡΠ΅Ρ ΠΏΠΎΠ½ΠΈΠΌΠ°Π½ΠΈΡ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ ΠΆΠΈΠ·Π½ΠΈ ΠΈ ΠΎΠ±ΠΈΡΠ°Π½ΠΈΡ Π»Π΅ΠΎΠΏΠ°ΡΠ΄ΠΎΠ². Π Π΄ΠΎΠΏΠΎΠ»Π½Π΅Π½ΠΈΠ΅ ΠΊ ΡΡΠΎΠΌΡ Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠΎ ΠΏΡΠΈΠΌΠ΅Π½ΡΡΡ ΠΌΠ΅ΡΡ ΠΏΠΎ Π·Π°ΡΠΈΡΠ΅ ΠΎΠΊΡΡΠΆΠ°ΡΡΠ΅ΠΉ ΡΡΠ΅Π΄Ρ, ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎ Π² ΡΠ΅Ρ
ΠΌΠ΅ΡΡΠ°Ρ
, Π³Π΄Π΅ ΡΠΎΡΡΠ΅Π΄ΠΎΡΠΎΡΠ΅Π½a Π±ΠΎΠ»ΡΡΠ°Ρ ΠΏΠΎΠΏΡΠ»ΡΡΠΈΡ ΡΡΠΈΡ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
, Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠΎ ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΠΎΠ²Π°ΡΡ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ ΡΠ΅Π»ΡΡΠΊΠΎΠ³ΠΎ Ρ
ΠΎΠ·ΡΠΉΡΡΠ²Π° Π² Π΄Π°Π½Π½ΡΡ
ΠΎΠ±Π»Π°ΡΡΡΡ
, ΠΏΡΠΈΠ²Π»Π΅ΠΊΠ°ΡΡ Π²Π½ΠΈΠΌΠ°Π½ΠΈΠ΅ Π»ΡΠ΄Π΅ΠΉ ΠΊ Π΄Π°Π½Π½ΠΎΠΉ ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ΅ Π·Π° ΡΡΠ΅Ρ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΈ Π² Π²ΠΈΠ΄Π΅ Π²ΡΠ²Π΅ΡΠΎΠΊ, ΠΎΠ³ΡΠ°Π½ΠΈΡΠΈΠ²Π°ΡΡ ΡΠΊΠΎΡΠΎΡΡΡ Π΄Π²ΠΈΠΆΠ΅Π½ΠΈΡ ΡΡΠ°Π½ΡΠΏΠΎΡΡΠ°, ΡΡΠΎΠ±Ρ ΡΠ½ΠΈΠ·ΠΈΡΡ ΠΏΡΠΎΡΠ΅Π½Ρ Π³ΠΈΠ±Π΅Π»ΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Large schwannoma in the ulnar nerve in axilla - A case report
Introduction: Schwannomas are nerve sheath tumours which majority is benign. Only one case of solitary schwannoma in the ulnar branch of brachial plexus in the axilla is so far reported. Some are asymptomatic while others having pain and neurological disability. For diagnosis, imaging studies such as ultrasonography, computerized tomography, magnetic resonance imaging, electromyography and aid of electron microscopy and immunohistochemistry are used. Non-surgical treatment is appropriate for slow growing and asymptomatic tumours. Surgical treatment is aimed to prevent progressive neurological deficit. Damage to the parent nerve is a known complication.Case report: A 44 year old female was investigated for a lump in her left axilla. Ultrasonography showed well defined hypoechoic solid mass, suspicious of an enlarged lymph node in the axilla with normal breasts. Tru-cut biopsy was compatible with histological appearance of schwannoma. A large (7.0x5.5x4.0cm3) well circumcised white mass of tissue arising at the origin of ulnar nerve deep in the left axilla was enucleated. Histologically lesion was compatible with a schwannoma with an intact capsule. Diffusely positive S-100 protein was demonstrated immunohistochemically and malignancy excluded. Post-operatively patient had ulnar nerve palsy confirmed by nerve conduction studies. Hand physiotherapy was offered and nerve function gradually improved.Discussion: Benign schwannoma of the ulnar nerve is not a common condition encountered in general clinical practice, hence delay in diagnosis is of concern. MRI and histology is needed to diagnose schwannoma accurately and to exclude malignancy. Treatment is complete excision while preserving nerve function preferably under intra-operative electrophysiological guidance.
Anthelmintic efficacy of albendazole and levamisole against gastrointestinal parasites in sri lanakan elephants at udawalawe, Sri Lanka
Π°Π»ΡΠ±Π΅Π½Π΄Π°Π·ΠΎΠ», Π»Π΅Π²Π°ΠΌΠΈΠ·ΠΎΠ», ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΠΎΠ΅ Π»Π΅ΡΠ΅Π½ΠΈΠ΅, ΡΡΡΠΎΠ½Π³ΠΈΠ»ΡΡΠ°, ΡΠ°ΡΡΠΈΠΎΠ»Ρ, ΠΏΠ°ΡΠ°Π·ΠΈΡΠΎΠ·Ρ, ΡΠ»ΠΎΠ½Ρ, Π¨ΡΠΈ ΠΠ°Π½ΠΊΠ°ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ Π°Π½ΡΠΈΠ³Π΅Π»ΡΠΌΠΈΠ½ΡΠ½ΠΎΠΉ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Β«ΠΠ»ΡΠ±Π΅Π½Π΄Π°Π·ΠΎΠ»Β» ΠΈ Β«ΠΠ΅Π²ΠΎΠΌΠΈΠ·ΠΎΠ»Β» ΠΏΡΠΈ ΠΆΠ΅Π»ΡΠ΄ΠΎΡΠ½ΠΎ-ΠΊΠΈΡΠ΅ΡΠ½ΡΡ
ΠΏΠ°ΡΠ°Π·ΠΈΡΠΎΠ·Π°Ρ
ΡΠ»ΠΎΠ½ΠΎΠ² Π¨ΡΠΈ-ΠΠ°Π½ΠΊΠΈ. ΠΠ΅ΡΠ²ΡΠΉ ΡΠ΅ΡΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΡΡ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΠΎΠ»ΡΠΊΠΎ Π°Π»ΡΠ±Π΅Π½Π΄Π°Π·ΠΎΠ»Π°, Π²ΡΠΎΡΠΎΠΉ ΡΠ΅ΡΡ β ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΠΎΠ΅ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ². Π‘Π»ΠΎΠ½Ρ, Π½Π°Ρ
ΠΎΠ΄ΡΡΠΈΠ΅ΡΡ Π² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ΅, Π±ΡΠ»ΠΈ ΠΈΠ½Π²Π°Π·ΠΈΡΠΎΠ²Π°Π½Ρ ΡΠ°ΡΡΠΈΠΎΠ»Π°ΠΌΠΈ ΠΈ ΡΡΡΠΎΠ½Π³ΠΈΠ»ΡΡΠ°ΠΌΠΈ. ΠΡΠ»ΠΎ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΠΎΠ΅ Π»Π΅ΡΠ΅Π½ΠΈΠ΅ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎ ΠΏΡΠΎΡΠΈΠ² ΠΆΠ΅Π»ΡΠ΄ΠΎΡΠ½ΠΎ-ΠΊΠΈΡΠ΅ΡΠ½ΡΡ
Π³Π΅Π»ΡΠΌΠΈΠ½ΡΠΎΠ² ΡΠ»ΠΎΠ½ΠΎΠ²
Development and validation of a reference marker for identification of aerobic and anaerobic bacteria associated with diabetes chronic wound ulcers using PCR denaturing gradient gel electrophoresis
Introduction: Diabetes chronic wounds consist with a diverse microbial community and unculturablespecies may be highly prevalent.Objectives: This study aimed to establish a bacterial reference marker consisting of a group ofchronic wound related bacteria, using polymerase chain reaction-denaturing gradient gelelectrophoresis (PCR-DGGE) for profiling of bacteria in diabetes chronic wound infections.Methods: DNA was extracted from the known wound bacterial strains. PCRβDGGE was performedusing eubacterial specific primers targeting V2-V3 region of 16S rDNA. DGGE was performed usinga 30-55% denaturing gradient. Migration position of each organism was detected on DGGE gel andimportant organisms were selected. Equal volume from PCR products of each selected organism wasmixed, diluted with gel loading dye in 1:1.5 ratio and used for all DGGE gels. The ladder was thensubjected to species identification of fifteen tissue debridement specimens obtained from diabeteschronic wound ulcers. The identification efficacy was tested by sequencing.Results: DNA of bacterial pathogens which showed different migration distances on the gel werecombined and used as a reference panel. This bacterial ladder consisted of eleven different bacterialspecies including Bacteroides sp., S. aureus, Acineto bacter sp., P. aeruginosa, Streptococcus Group Aand Group B sp., E. faecalis, Providencia sp., Veillonella sp., E .coli and Enterobacter sp. Accordingto the reference panel, Pseudomonas species were abundant. Further the results were confirmed bysequencing.Conclusion: Reference marker allows comparative analysis of DGGE patterns and can be used as atool for presumptive identification of polymicrobial microbiota in chronic wound infections
Human stomach microbiota: Effects on health and disease
The gut microbiota is a complex ecological community, consisting of trillions of microbes which include bacteria, viruses, fungi and protozoa. The stomach was previously considered as a sterile site uninhabited by microbes due to its hostile environmental conditions. Breaking this concept, Helicobacter pylori was the first pathogen reported to inhabit the stomach. Recent studies have suggested that the stomach harbours transient as well as certain commensal bacterial and fungal species. The five major microbial phyla in the stomach have been identified as Firmicutes, Bacteroidetes, Actinobacteria, Fusobacteria and Proteobacteria.Β The composition of gastric microbiota is dynamic and is affected by several factors.Β These include age group, dietary habits, medication use, inflammation of gastric mucosa and H. pylori colonization.Β Further, the role of host genetics has recently been studied in maintaining the stomach microbiota. Mutations in host genes may affect the hostβs immune response towards commensal bacteria and reduce their number and diversity.Β The essential multiple roles of gut microorganisms include maintaining homeostasis in the gut, contributing to immune function and extraction of nutrients and energy from our diets.Β Loss of the normal balance between the gut microbiota and host has been associated with several abnormal conditions and disorders such as obesity, malnutrition, inflammatory bowel diseases (IBD), neurological disorders, and cancer. In the stomach, the interaction between H. pylori and the gastric microbiota can also influence gastric disΒease progression. Further studies should focus on addressing the role of gastric dysbiosis in health and disease. Identifying gastric microbiota is essential to understand how the gut microbiota and H. pylori affect health and disease.</p
Influence of culture medium on in-vitro biofilm formation by Candida species
Objectives:Β Objective of this study was to establish an in vitro biofilm on the 96 well plates and to determine the efficacy of three different culture media on biofilm formation of Candida albicans and C. tropicalisΒ Methods:Β A 96 well sterile, polystyrene plate was inoculated using 10^6 cell/ml of C. albicans and C. tropicalis suspensions and the growth rate of planktonic cells was determined by measuring the absorbance (OD492) at 2 hour intervals. Adhesion of Candidial cells to initiate the biofilm formation in the presence of three culture media (Yeast Nitrogen Base (YNB) supplemented with 100 mM glucose, Sabouraud Dextrose Broth (SDB) and RPMI1640) was quantified using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and Crystal Violet (CV) assay after 90 minutes. Biofilms of C. albicans, C. tropicalis and 1:1 co-biofilms were developed and the growth rates were quantified at 24 hoursβ time intervals. Scanning electron microscope (SEM) was performed to assess the architecture.Β Results:Β Planktonic cells of both C. albicans and C. tropicalis showed maximum growth with SDB. C. albicans and co-biofilm adhesion were significantly facilitated with RPMI1640 and the best medium for C. tropicalis adhesion was YNB. Biofilms showed the maximum growth rate in RPMI 1640. C. tropicalis exhibited the minimum growth with all three culture media.Conclusions:Β The maximum growth rate for planktonic C. albicans and C. tropicalis was achieved with SDB. However RPMI 1640 was the best medium for growth of biofilms
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