Induksi Kalus Tiga Kultivar Lili dari Petal Bunga pada Beberapa Jenis Media

ABSTRACTLilium is usually propagated vegetatively by using bulb scales  or bulbs. Based on the totipotency ability  of  every  parts  of  plant,  it  is  possible  to  regenerate  them  into  plantlets.  The  objective  of  the experiment was to find out micropropagation technique of lily  by  using petal  of  three cultivars of liliumas explants. Different stages  of developing flower buds was used in this research. The best medium for callus induction was Murashige & Skoog (MS)  + 0.3  mg  l-1TDZ. The stage of flower bud  development D2  (diameter flower bud was 2.15 cm and 7 cm  in  length) of  ‘Frutty Pink’  cultivar  was  more responsiveon callus induction media than other cultivars (‘Sorbon’ and ‘Purple Diamond’).Key words: TDZ (thidiazuron),lilium, callus induction, flower, explant.ABSTRAKLilium  biasanya  diperbanyak  secara  vegetatif  dengan  umbi  sisik  atau bulb.  Berdasarkan  pada kemampuan totipotensi dari setiap bagian tanaman, dimungkinkan untuk meregenerasikan tanaman dari berbagai bagian tanaman menjadi planlet. Tujuan percobaan adalah menemukan teknik mikropropagasi lili  menggunakan  petal  tiga  kultivar  lili  sebagai  eksplan.  Stadia  perkembangan  bunga  yang  berbeda digunakan  dalam  penelitian  ini.  Media  yang  terbaik  untuk  induksi  kalus  adalah  media  Murashige  & Skoog (MS) +0.3 mg  l-1TDZ. Stadia perkembangan tunas bunga D2  dengan diametertunas bunga 2.15 cm  dan panjang bunga 7 cm  dari kultivar  ‘Frutty Pink’  lebih responsif dalam induksi   kalus dibanding kultivar lainnya (‘Sorbon’ dan ‘Purple Diamond’).Kata kunci: TDZ (thidiazuron), Lilium, induksi kalus, bunga, eksplan

Kombinasi persilangan dan seleksi in vitro untuk mendapatkan kultivar unggul kentang

Cultivars Granola  dan Atlantic are the best cultivars so far for farmers, due to it adaptation in Indonesia. Cv Granola is resistant to several important diseases, but the quality is low, thus only produce for vegetable purposes. Cv Atlantic is the best cultivar, in term of productivity and quality. Cv Atlantic is suitable for industry purposes, susceptible to several important diseases. Crossing between both cultivars expected to produce high yielding cultivars and adapted to Indonesian environment. The method used was crossing and germinated the seed then selected in vitro. Each germinated seed was propagated in vitro and considered as clones. In vitro selection was performed through vigour, bacterial wilt and soft rot test, as well as micro tuber production. The result of selectionwas then verified in the field. From thousands of seed germinated producing 12 selected clones, 7 clones of them were showed better then their parent in term of tuberization and micro tuber production. The clones were Atnola 1, Atnola 5, Atnola 3, Atnola 10, Atnola 12, Atnola 24, and Atnola 26. Four clones showed level of resistance better than the parent, namely Atnola 3, Atnola 5, Atnola 8, and Atnola 10. The result of field test showed that the seven clones produced better tuber than the parent Atlantic dan Granola, which were Atnola 5 dan Atnola 10 showed vigour, tuber weight, and better level of resistance, thus will be the candidate of high yielding cultivars