128 research outputs found
Deleterious effects of xanthine oxidase on rat liver endothelial cells after ischemia/reperfusion
AbstractPrevious studies have demonstrated that reactive oxygen species are involved in ischemic injury. The present work was undertaken to determine in vivo the role of xanthine oxidase in the oxygen free radical production during rat liver ischemia and to examine the activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase) during the same period. Our results indicate a 4-fold increase in xanthine oxidase activity between 2 and 3 hours of normothermic ischemia, in parallel with a decrease in cell viability. Moderate hypothermia delays both events. Under the same conditions, the activity of oxygen radical scavenging enzymes remains unchanged. Moreover, we have compared in vitro the susceptibility of isolated liver cells to an oxidative stress induced by O2·−, H2O2 and OH. Our results reveal that endothelial cells are much more susceptible to reactive oxygen species than hepatocytes, probably because they lack H2O2-detoxifying enzymes. These findings suggest that xanthine oxidase might play a major role in the ischemic injury mainly at the level of the sinusoidal space where most endothelial cells are located
Biological functions of lysosomal membrane-associated glycoproteins
Lysosomes are the final repository of degradation products from the extracellular and intracellular spaces. The lysosomal membrane forms a unique vacuole participating in both endocytosis and autophagocytosis. It is extremely resistant to degradation by lysosomal hydrolases, maintains an acidic intralysosomal environment, transports amino acids and oligosaccharides produced by lysosomal hydrolases, interacts and fuses with other membrane organelles, such as endosomes and phagosomes, and with the plasma membrane.Biomedical Reviews 1997; 8: 119-125
Rat liver membrane preparations
The quality of rat liver plasma membranes was examined using three different methods of preparation. Hypotonic Ca++-free preparation media were compared with hypotonic Ca++ media and with isotonic Ca++ media. The plasma membranes prepared in hypotonic Ca++ free media were as pure or better than the other two preparation types, while the (Na+K+)-MgATPase, MgATPase, and the ADPase specific activities were higher. The AMPase specific activities were of similar value for all three types of preparation.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/34040/1/0000317.pd
Diethanolamine-induced alteration of hepatic mitochondrial function and structure
Diethanolamine (DEA) has been shown to interfere with phospholipid metabolism. It was hypothesized that DEA may alter membrane-bound biochemical processes which are dependent upon phospholipids. DEA was investigated for its effect on hepatic mitochondrial function and structure in the male Sprague-Dawley rat. DEA did not produce mitochondrial alterations in in vitro preparations from liver tissue. Similarly, acute treatment in vivo was without effect. However, a loss of mitochondrial integrity developed after subacute administration of DEA. Rats were given 0.25 (42 mg/kg/day), 1.0 (160 mg/kg/day), or 3.0 (490 mg/kg/day) mg/ml in their drinking water for varying periods up to 5 weeks. The effects on mitochondria were dose- and time dependent. Significant alterations appeared after 3 days at 3.0 mg/ml and after 1 week at 1.0 mg/ml. Mitochondrial State 4 activity was significantly elevated at all three dose levels following 2 weeks of DEA treatment. Concomitantly, an increase was noted in the Mg2+-dependent ATPase activity from rats treated with DEA at 2.0 mg/ml for 3 weeks. Electron micrographs indicated that treated animals had swollen hepatic mitochondria. The changes in the organelle may be related to alteration of phospholipid metabolism by DEA.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/23610/1/0000572.pd
The stability of structures containing endocytosed horseradish peroxidase in the presence of glycyl-phenyIalanine-2-naphthylamide
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