Analysis of attitudes towards social services expressed by the citizens of Arlington, Massachusetts in the Citizens' Survey of 1980

Thesis (M.C.P.)--Massachusetts Institute of Technology, Dept. of Urban Studies and Planning, 1981.MICROFICHE COPY AVAILABLE IN ARCHIVES AND ROTCH.Bibliography: leaf 158.by Jeffrey Alan Watiker.M.C.P

Structure-Function Study of the Lab Opossum Prostate

The mammalian prostate gland secretes fluids that, along with sperm from the testes and fluids from other glands, comprise semen. While the structure and function of the prostate is well known in eutherians, the anatomy of the prostate gland in metatherian (marsupial) mammals is poorly studied. My goal is to contribute to the knowledge regarding prostate anatomy as a way of understanding the evolution of internal fertilization as an effective reproductive strategy by exploring the homologous and analogous aspects of this organ in metatherians versus eutherians. Through dissections of male laboratory opossums and rats (M. domestica and R. norvegicus), I collected data on their gross prostate anatomy. The rat prostate consists of three lobes, dorsal, lateral, and ventral arranged around the base of a short urethra. The opossum prostate consists of three regions, S1, S2, and S3 that wrap around a much longer urethra. Using histological analysis, I compared their microanatomy to establish homologies when possible. I used immunochemistry to localize the three proteins that are produced in the three different lobes of the rat prostate. Detecting the presence similarly in distinct sections of the opossum prostate would provide insight into the functional homology of this organ as well as provide evidence that prostate genes are conserved in both species. I found that while the pattern of immunostaining for each protein differed between S1, S2, and S3, the staining was not as localized as the lobes of the rat prostate. This suggests that the way in which the sections of the opossum prostate function differ from the lobes of the rat prostate despite the conservation of genes

Designing the CRISP Document Format: A Secure Declarative Language for the Web

The contemporary web forces developers and users to mitigate numerous vulnerabilities to security and privacy. In this study, I explore how the CRISP Document Format (CDF) project focuses on designing a fundamentally secure language for the web, and thereby frees these parties from addressing each issue one by one. CDF is a fully declarative client-side language for creating web pages where sites are developed from a finite set of whitelisted static and dynamic elements. As a result of its design, a CDF site holds privacy guarantees for the client while also relieving developers of the burden of building and maintaining the security of their sites

Mutation in the sixth immunoglobulin domain of L1CAM is associated with migrational brain anomalies.

ObjectiveTo describe the phenotype of a patient with classical features of X-linked L1 syndrome associated with novel brain malformations.MethodsDiagnostic analysis included physical and dysmorphology examinations, MRI of the brain, and exome sequencing of the family trio.ResultsWe report a 2.5-year-old boy with developmental delay, dysmorphic facies, and adducted thumbs. MRI of the brain showed a truncated corpus callosum and periventricular heterotopias associated with polymicrogyria (PMG). Variant segregation analysis with exome sequencing discovered a novel maternally derived hemizygous variant in exon 14 of the L1CAM gene (c.1759 G>C; p.G587R).ConclusionsThis novel L1CAM mutation was located in the protein's sixth immunoglobin domain and involved glycine-587, a key residue in the structure of L1CAM because of its interactions with lysine-606, which indicates that any mutation at this site would likely affect the secondary structure and function of the protein. The replacement of the small nonpolar glycine residue with a large basic arginine would have an even more dramatic result. The presentation of periventricular nodular heterotopias with overlying PMG is very uncommon, and its association with L1CAM may provide insight into other similar cases. Furthermore, this presentation indicates the important role that L1CAM plays in neuronal migration and brain development and extends the phenotype associated with L1CAM-associated disorders

Skeletal dysplasia with bowing long bones: Proposed flowchart for prenatal diagnosis with case demonstration

Objective: Skeletal dysplasia with bowing long bones is a rare group of multiple characterized congenital anomalies. Materials and Methods: We introduce a simple, practical diagnostic flowchart that may be helpful in identifying the appropriate pathway of obstetrical management. Results: Herein, we describe four fetal cases of bent bony dysplasia that focus on ultrasound findings, phenotype, molecular tests, distinctive X-ray features, and chondral growth plate histology. The first case was a typical campomelic dysplasia resulting from a de novo mutation in the SOX9 gene. The second fetus was affected by osteogenesis imperfecta Type II carrying a mutation in the COLA1 gene. The third case was a rare presentation of campomelic dysplasia, Cumming type, in which SOX9 examination was normal. Subsequently, a femoral hypoplasia unusual facies syndrome is also discussed. Conclusion: Targeted molecular tests and genetic counseling are required for supplementing ultrasound imaging in order to diagnose the correct skeletal disorders