The Adaptive Function of Masturbation in a Promiscuous African Ground Squirrel

Background: Studies of animal mating systems increasingly emphasize female multiple mating and cryptic sexual selection, particularly sperm competition. Males under intense sperm competition may manipulate sperm quantity and quality through masturbation, which could waste sperm and decrease fertility. I examined the factors influencing masturbation by male Cape ground squirrels (Xerus inauris) in light of a number of functional hypotheses. Methodology: Observational data on a marked population of squirrels were collected in east-central Namibia using scan and all-occurrences sampling. Findings: Masturbation was far more frequent on days of female oestrus and mostly occurred after copulation. Masturbation rates were higher in dominant males, which copulate more, than in subordinates and increased with number of mates a female accepts. Conclusions: These results suggest that masturbation in this species was not a response to sperm competition nor a sexual outlet by subordinates that did not copulate. Instead masturbation could function as a form of genital grooming. Female Cape ground squirrels mate with up to 10 males in a 3-hr oestrus, and by masturbating after copulation males could reduce the chance of infection. Sexually transmitted infections (STIs) can profoundly affect fertility, and their consequences for mating strategies need to be examined more fully

Diatom communities differ among Antarctic moss and lichen vegetation types

Continental Antarctica is a polar desert containing sparse pockets of vegetation within ice-free areas. Despite the recognized association between lichens, mosses and epiphytic diatoms, the environmental factors controlling diatom community structure are poorly understood. We investigated the association between diatom communities and host vegetation characteristics by experimentally adding nutrients and/or water to two bryophyte (healthy and moribund) and two lichen (crustose and Usnea) vegetation types in the Windmill Islands. Diatom communities were morphologically characterized, diversity indices calculated and differences between treatments, vegetation type and vegetation characteristics tested. We identified 49 diatom taxa, 8 of which occurred with \u3e 1% relative abundance. Bryophyte and lichen vegetation harboured significantly different diatom communities, both in composition and diversity indices. Specifically, Luticola muticopsis was more prevalent in moribund bryophytes and crustose lichens, and Usnea lichens showed lower species richness than other types. While nutrient and water additions did not significantly alter diatom communities, diversity indices and some species showed relationships with vegetation physiological characteristics, notably %N and δ13C, suggesting the importance of ambient gradients in water and nutrient availability. Collectively, this work suggests that future conditions favouring the dominance of a particular vegetation type may have a homogenizing effect on the terrestrial diatom communities of East Antarctica

Trade-offs between immunity and testosterone in male African ground squirrels

The immunocompetence handicap hypothesis (ICHH) proposes that testosterone has both beneficial effects on male reproductive potential and negative effects by suppressing the immune system. However, support for the ICHH has been variable and an alternative hypothesis suggests that testosterone may be acting indirectly via cortisol to suppress immunity (the stress-linked ICHH). A third hypothesis is that increased energetic investment in immunity results in the suppression of testosterone. We tested these hypotheses in male Cape ground squirrels (Xerus inauris) through two separate manipulations; first by triggering a strong immune response using a lipopolysaccharide (LPS) injection and secondly by increasing circulating testosterone using silastic testosterone implants. Responding to an immune challenge significantly reduced testosterone, supporting the immunity suppression hypothesis, while increasing circulating testosterone had no effect on immunocompetence, body mass, ectoparasite abundances or cortisol levels, failing to support either the ICHH or stress-linked ICHH. Our results add to the increasing body of literature that challenges the ICHH, and we conclude that the trade-off between testosterone and immunity is mediated through immune activation and not through testosterone in male Cape ground squirrels. Being able to test the ICHH, stress-linked ICHH and immune suppression hypotheses in a free ranging mammal gives us a unique opportunity to examine the mechanisms mediating this trade-off.A Natural Sciences and Engineering Research Council of Canada Discovery grant (#04362), Canadian Foundation of Innovation (#27125), and a University of Manitoba Faculty of Science Field Work Support grant awarded to JMW, a Natural Sciences and Engineering Research Council of Canada Discovery grant (#311909) awarded to WGA, SARChI chair (# 64756) from the Department of Science and Technology and National Research Foundation, RSA awarded to NCB and awards from the University of Manitoba (Barrett- Hamilton Award; Faculty of Science) to KAO.http://jeb.biologists.org2019-08-23am2018Mammal Research InstituteZoology and Entomolog

Lovers, not fighters : docility influences reproductive fitness, but not survival, in male Cape ground squirrels, Xerus inauris

Over their lifetime, individuals may use different behavioural strategies to maximize their fitness. Some behavioural traits may be consistent among individuals over time (i.e., ‘personality’ traits) resulting in an individual behavioural phenotype with different associated costs and benefits. Understanding how behavioural traits are linked to lifetime fitness requires tracking individuals over their lifetime. Here, we leverage a long-term study on a multi-year living species (maximum lifespan ~ 10 years) to examine how docility (an individual’s reaction to trapping and handling) may contribute to how males are able to maximize their lifetime fitness. Cape ground squirrels are burrowing mammals that live in social groups, and although males lack physical aggression and territoriality, they vary in docility. Males face high predation risk and high reproductive competition and employ either of two reproductive tactics (‘natal’ or ‘band’) which are not associated with different docility personalities. We found that although more docile individuals sired more offspring on an annual basis, docility did not affect an individual’s long-term (lifetime) reproductive output. Survival was not associated with docility or body condition, but annual survival was influenced by rainfall. Our findings suggest that although docility may represent a behavioural strategy to maximize fitness by possibly playing a role in female-male associations or female mate-choice, variations in docility within our study population is likely maintained by other environmental drivers. However, individual variations in behaviours may still contribute as part of the ‘tool kit’ individuals use to maximize their lifetime fitness

Lovers, not fighters: docility influences reproductive fitness, but not survival, in male Cape ground squirrels, Xerus inauris

DATA AVAILABILITY : All data analyzed for this study are included in this published article and its supplementary information files.Over their lifetime, individuals may use different behavioural strategies to maximize their fitness. Some behavioural traits may be consistent among individuals over time (i.e., ‘personality’ traits) resulting in an individual behavioural phenotype with different associated costs and benefits. Understanding how behavioural traits are linked to lifetime fitness requires tracking individuals over their lifetime. Here, we leverage a long-term study on a multi-year living species (maximum lifespan ~ 10 years) to examine how docility (an individual’s reaction to trapping and handling) may contribute to how males are able to maximize their lifetime fitness. Cape ground squirrels are burrowing mammals that live in social groups, and although males lack physical aggression and territoriality, they vary in docility. Males face high predation risk and high reproductive competition and employ either of two reproductive tactics (‘natal’ or ‘band’) which are not associated with different docility personalities. We found that although more docile individuals sired more offspring on an annual basis, docility did not affect an individual’s long-term (lifetime) reproductive output. Survival was not associated with docility or body condition, but annual survival was influenced by rainfall. Our findings suggest that although docility may represent a behavioural strategy to maximize fitness by possibly playing a role in female-male associations or female mate-choice, variations in docility within our study population is likely maintained by other environmental drivers. However, individual variations in behaviours may still contribute as part of the ‘tool kit’ individuals use to maximize their lifetime fitness.The Natural Sciences and Engineering Research Council of Canada Discovery Grant, NSERC Small Research Equipment Grant, Canadian Foundation for Innovation and the Manitoba Research and Innovation Grant, University of Manitoba Faculty of Science Field Work Support Program funding, the University of Manitoba Research Grants Program, the Faculty of Science and an Oxford Brookes Emerging Leaders Research Fellowship.http://link.springer.com/journal/265hj2024Mammal Research InstituteZoology and EntomologyNon

Energetics and water flux in the subterranean rodent family Bathyergidae

The doubly labeled water (DLW) technique and indirect calorimetry enable measurement of an animal’s daily energy expenditure (DEE, kJ/day), resting metabolic rate (RMR, kJ/d), sustained metabolic scope (SusMS), body fat content (BF, %) as well as water turnover (WTO, ml/day), and water economy index (ml/kJ). Small mammals have been the primary focus of many of the DLW studies to date. From large multi-species analyses of the energetics and water flux of aboveground small mammals, well-defined trends have been observed. These trends mainly refer to an adaptive advantage for lower RMR, DEE, SusMS, WTO and WEI in more ariddwelling animals to increase water and energy savings under low and unpredictable resource availability. The study of the subterranean rodent family Bathyergidae (African mole-rats) has been of particular interest with regards to field metabolic rate and metabolic studies. Although a great deal of research has been conducted on the Bathyergidae, a complete overview and multispecies analysis of the energetics and water flux of this family is lacking. Consequently, we assessed DEE, RMR, SusMS, BF, WTO and WEI across several different species of bathyergids from various climatic regions, and compared these to the established patterns of energetics and water flux for aboveground rodents. There was notable variation across the Bathyergidae inhabiting areas with different aridities, often contrary to the variations observed in above-ground species. These include increased DEE and WEI in arid-dwelling bathyergid species. While the climate was not a clear factor when predicting the SusMS of a bathyergid species, rather the degree of group living was a strong driver of SusMS, with solitary species possessing the highest SusMS compared to the socially living species. We conclude that the constraints of the underground lifestyle and the consequent spectrum of social behaviors possessed by the family Bathyergidae are most likely to be more crucial to their energetics and water flux than their habitat; however other important unstudied factors may still be at play. More so, this study provides evidence that often unreported parameters, measured through use of the DLW technique (such as BF and WEI) can enable species to be identified that might be at particular risk to climate change.The SARChI Chair of Mammal Behavioral Ecology and Physiology, National Research Foundation RSA, the British Ecological Society (SEPG), and the National Science Foundation, United States.http://frontiersin.org/Ecology_and_Evolutiondm2022Zoology and Entomolog

String Matching and 1d Lattice Gases

We calculate the probability distributions for the number of occurrences $n$ of a given $l$ letter word in a random string of $k$ letters. Analytical expressions for the distribution are known for the asymptotic regimes (i) $k \gg r^l \gg 1$ (Gaussian) and $k,l \to \infty$ such that $k/r^l$ is finite (Compound Poisson). However, it is known that these distributions do now work well in the intermediate regime $k \gtrsim r^l \gtrsim 1$. We show that the problem of calculating the string matching probability can be cast into a determining the configurational partition function of a 1d lattice gas with interacting particles so that the matching probability becomes the grand-partition sum of the lattice gas, with the number of particles corresponding to the number of matches. We perform a virial expansion of the effective equation of state and obtain the probability distribution. Our result reproduces the behavior of the distribution in all regimes. We are also able to show analytically how the limiting distributions arise. Our analysis builds on the fact that the effective interactions between the particles consist of a relatively strong core of size $l$, the word length, followed by a weak, exponentially decaying tail. We find that the asymptotic regimes correspond to the case where the tail of the interactions can be neglected, while in the intermediate regime they need to be kept in the analysis. Our results are readily generalized to the case where the random strings are generated by more complicated stochastic processes such as a non-uniform letter probability distribution or Markov chains. We show that in these cases the tails of the effective interactions can be made even more dominant rendering thus the asymptotic approximations less accurate in such a regime.Comment: 44 pages and 8 figures. Major revision of previous version. The lattice gas analogy has been worked out in full, including virial expansion and equation of state. This constitutes the main part of the paper now. Connections with existing work is made and references should be up to date now. To be submitted for publicatio

Immunological Sex Differences in Socially Promiscuous African Ground Squirrels

Differences in how males and females respond to foreign antigens are common across taxa. Such sexual differences in the immune system are predicted to be greater in species with high promiscuity and sociality as these factors increase the likelihood of disease transmission. Intense sperm competition is thought to further this sexual dichotomy as increased investment in spermatogenesis likely incurs additional immunological costs. Xerus inauris, a ground squirrel found throughout southern Africa, is extremely social and promiscuous with one of the highest male reproductive investments among rodents. These life-history attributes suggest males and females should demonstrate a large dichotomy in immunity. Contrary to our prediction, we found no difference in spleen mass between the sexes. However, we did find significant biases in leukocyte types and red blood cell counts, possibly reflecting responses to parasite types. Among males, we predicted greater investments in spermatogenesis would result in reduced immunological investments. We found a negative association between testes and spleen size and a positive relationship between testes and number of lice suggesting trade-offs in reproductive investment possibly due to the costs associated with spermatogenesis and immunity. We suggest when measuring sexual differences in immunity it is important to consider the effects of reproductive pressures, parasite types, and life history costs