Candida albicans Berthout, 1923: Hydrolases activity and own method of digestive tract strains biotyping

112 strains of Candida albicans were isolated from oral cavity and ontocenoses of the upper digestive tract (endoscopy) of children (age: 5-17) with gastrointestinal disorders. Axenic strains were differentiated with API 20C AUX and API ZYM tests (bioMerieux). Then enzymograms and biotypes were determined for all the strains based on the activity of 19 hydrolases. The highest activity was noted for: e₂ - phosphatase alcaline, e₆ – leucine arylamidase, e₁₁ - phosphatase acid, e₅ - lipase (c₁₄); e₇ - valine arylamidase, e₁₂ - naphtol-AS-BI-phosphohydrolase, e₁₆ - α-glucosidase and e₁₈ - N-acetyl-ß-glucosamidase, and the latter four were used for biotyping procedures. Our own system was based on the mathematical binominal distribution formula (1: 4: 6: 4: 1): all „+”; one „ -” three „+”; two „-”, two ,,+”; tbree „-”, one „+”; all „-”. We bave found the following biotypes: A (16.1 ± 3.5%), B₁ (2.7 ± 2.53%), B₃ (8.0 ± 25%), B₄ (22.3 ± 3.9%), C₂ (1.8 ± 1.3%), C₃ (7.1 ± 2.4%), C₆ (30.4 ± 4.3%), D₃ (11.6 ± 3.0%)

Wrazliwosc na ketokonazol szczepow Candida zasiedlajacych ontocenozy przewodu pokarmowego dzieci

The study included 52 children. During endoscopy the contents of oesophagus, stomach and duodenum were collected with catheters, and additionally washings from the oral cavity and anal swabs or samples of faeces. Materials were inoculated on Sabouraud's media. The axenic strains of fungi were identified using own methods and others tests (API 20C, API 20C AUX). The activity of ketoconazole (K) was measured against 100 strains of Candida: C. albicans (91 strains), C. kefyr (4), C. famata (2). C. tropicalis (1), C. guilliermondii (1), C. glabrata (1). The strains susceptibility to K (RO4 1400 Janssen) was estimated with the own system based on the dose - response curves; the minimal inhibitory concentration (MIC) values were canculated. The low values of MIC indicate high susceptibility to K of examined Candida strains

Wlasciwosci biochemiczne grzybow izolowanych z ontocenoz przewodu pokarmowego dzieci

Fermentation of carbohydrates and assimilation of carbon compounds were evaluation. The utilization of 18 specific carbon compounds was estimated in the investigation of each strains for differentiation of the species. From among 125 strains of fungi the following were found: Candida albicans ( 112 strain), C. famata (2), C. glabrata (1). C. guilliermondii (1), C. kefir (4), C. tropicalis (2). The activity of 19 hydrolases was investigated using API ZYM. Biotyping of Candida strains was done according to the Williamson classification ( 1986), modified by Kurnatowska (1998). All strains were isolated from the oral cavity, rectum, as well as from the materials collected during endoscopy in children

Inwazje grzybami przewodu pokarmowego dzieci

The aim of presented study were fungal invasions of the oral cavity and gastrointestinal (GI) tract in children suspected of the inflammation of gastric and duodenal mucosa and of absorption disturbance. The fungal strains (125) were identified using API 20C and API 20C AUX (bio Me'rieux); they were as follows: Candida albicans, C. famata, C. glabrata, C. guillermondii, C. kefyr and C. tropicalis