Geminiviruses: genome organization and protein functions

International audienc

Geminiviruses : genome organization and protein functions

International audienc

Geminiviruses : genome organization and protein functions

International audienc

Single crystal structure, solid state characterization and dissolution rate of terbinafine hydrochloride

Terbinafine hydrochloride (TH), a poorly water soluble antifungal agent, was characterized by solid state techniques including differential scanning calorimetry, thermogravimetry, X-ray powder diffraction, optical and electron microscopies, Fourier transform infrared, Raman and solid-state nuclear magnetic resonance spectroscopies and intrinsic dissolution rate (IDR). A colorless single crystal of TH was grown from an ethanol:water solution and its crystalline structure was determined through X-ray single crystal diffraction. Also, a new crystal habit of TH was obtained through the slow solvent evaporation technique revealing a needle-like shape. A comparison between the IDR results for the TH raw material and TH needle-like crystal revealed lower values for the new crystal habit, which can be attributed to the preferential orientation of the crystals in the compressed disks.Fil: Kuminek, Gislaine. Universidade Federal de Santa Catarina; BrasilFil: Rauber, Gabriela Schneider. Universidade Federal de Santa Catarina; BrasilFil: Riekes, Manoela Klüppel. Universidade Federal de Santa Catarina; BrasilFil: Campos, Carlos Eduardo Maduro de. Universidade Federal de Santa Catarina; BrasilFil: Monti, Gustavo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; ArgentinaFil: Bortoluzzi, Adailton João. Universidade Federal de Santa Catarina; BrasilFil: Cuffini, Silvia Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidade Federal de Santa Catarina; BrasilFil: Cardoso, Simone Goncalves. Universidade Federal de Santa Catarina; Brasi

Satellite DNA beta overrides the pathogenicity phenotype of the C4 gene of tomato leaf curl virus but does not compensate for loss of function of the coat protein and V2 genes

We have investigated the ability of satellite DNA β to complement mutations in the CP, V2 and C4 genes of the monopartite begomovirus, tomato leaf curl virus, which are potentially involved in movement. A mutation in the coat protein was not complemented by DNA β. Mutations of the C4 and V2 genes attenuated and abolished symptoms, respectively. In the presence of the C4 mutant, but not the V2 mutant, DNA β induced typical symptoms, confirming that the satellite encodes a dominant symptom determinant. In contrast to the C4 mutant, DNA β did not enhance the viral DNA levels of the V2 mutant, suggesting that V2 is required for this phenomenon. The significance of these findings is discussed based on our present understanding of the functions of the viral genes and DNA β.M. Saeed, S. Mansoor, M. A. Rezaian, R. W. Briddon and J. W. Randle