26 research outputs found
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Identification of host odour attractants for tsetse flies. Final report 1986-1990
Tsetse flies, Glossina spp., are blood-feeding insects and vectors of trypanosomes, microorganisms which cause sleeping sickness in man and a similar disease, "nagana" in domestic animals. The economic importance of trypanosomiasis is the constraint it imposes on orderly rural development in Africa, leading to under-exploitation of infested land and over-exploitation and degradation of trypanosomiasis-free areas.
Traps and targets which attract tsetse flies and kill them could provide environmentally-acceptable, appropriate technology for monitoring and control of tsetse in Africa. Unbaited devices providing only visual attraction have proved effective in monitoring and control of riverine species of tsetse, but not the savannah species found in the fly belt of Malawi, Mozambique, Zambia and Zimbabwe covered by the EDF Regional Tsetse and Trypanosomiasis Control Project (RTTCP).
Previously, collaborative was begun between glossinologists of the Zimbabwe Department of Veterinary Services (DVS) and UK Tsetse Research Laboratory (TRL) and chemists at NRI. This brought together the experience of the DVS in the field, the experience of TRL in laboratory bioassay work, and the experience of NRI in using gas chromatography linked to electroantennography (GC-EAG) and chemical techniques to detect and identify insect behaviour-modifying chemicals. Tsetse attractants produced by host animals were identified and synthesised, and dispensing systems for these compounds devised. Traps and targets impregnated with insecticide, baited with these lures were shown to provide effective control of the savannah tsetse species, G. pallidipes and G. m. morsitans
Price of popularity
Michael Brooks (20/27 December 2008, p 16) and Richard Hammond (3 January, p 14) each ask a question that has occupied the scientific community in the UK for a long time: how can we effectively popularise science and reduce the reported decline of interest in science subjects at school?
We must not, however, forget science in the enthusiasm for communicating it. Science is an evidence-based practice: it depends on flexible individuals who can generalise and specialise when and as required.
Intense specialism can kill mass appeal. But history shows us science specialists with complex personalities who can make today’s celebrities
Electroantennogram responses of the stable fly, Stomoxys calcitrans, to components of host odour
Activation of three species of tsetse (Glossina spp.) in response to host derived stimuli
Responses of tsetse flies (Glossina spp.) to compounds on the skin surface of an ox: a laboratory study
Responses of female New World screwworm flies, Cochliomyia hominivorax, to coloured targets in the laboratory
The role of volatile semiochemicals in mediating host location and selection by nuisance and disease-transmitting cattle flies
The role of volatile semiochemicals in mediating the location and selection within herds of Holstein-Friesian heifers by nuisance and disease-transmitting cattle flies was investigated using coupled gas chromatography-electrophysiology (GC-EAG), coupled gas chromatography-mass spectrometry (GC-MS), electrophysiology (EAG), laboratory behaviour and field studies. Using volatile extracts collected by air entrainment from heifers in the Netherlands, a number of active peaks were located by coupled GC-EAG for Musca autumnalis (de Geer) (Diptera: Muscidae) and Haematobia irritans (L.) (Diptera: Muscidae). Volatile samples were also collected from two heifers in Denmark shown in previous counting experiments to differ significantly in their fly loads. Coupled GC-EAG using Ha. irritans antennae revealed differences in the EAG response to the samples, with additional EAG activity in the sample collected from the heifer with the lower fly load. To identify more EAG active compounds, volatiles were also collected from 48-h-old urine by air entrainment. In total, 23 compounds were located and identified by coupled GC-EAG and GC-MS. Further electrophysiological testing of these compounds with five fly species [M. autumnalis, Ha. irritans, Hydrotaea irritans (L.) (Diptera: Muscidae), Stomoxys calcitrans (L.) (Diptera: Musicidae) and Wohlfahrtia magnifica (Schiner) (Diptera: Sarcophagidae)] showed that only some of the compounds were physiologically active across the range of flies tested. These included 1-octen-3-ol, 6-methyl-5-hepten-2-one, (Z)-3-hexen-1-ol, naphthalene, and all EAG active compounds identified from urine. Compounds showing significant EAG activity were tested for behavioural activity using a wind-tunnel designed for measuring upwind night behaviour. At certain concentrations, 1-octen-3-ol, 6-methyl-5-hepten-2-one and 3-octanol increased upwind flight, whereas naphthalene, propyl butanoate and linalool reduced upwind flight. In field studies using small herds of heifers ranked according to their fly load, individual slow-release formulations of 1-octen-3-ol and 6-methyl-5-hepten-2-one, when applied to low and high fly loading heifers, reduced fly loads on these individuals. This study provides evidence for the hypothesis that the natural differential attractiveness within herds of Holstein-Freisian heifers, i.e. a single host species, for cattle flies is partly due to differences in volatile semiochemicals emitted from the host. It is suggested that this phenomenon applies to other vertebrate host species and their associated insect pests