Constraints on the mass of a habitable planet with water of nebular origin

From an astrobiological point of view, special attention has been paid to the probability of habitable planets in extrasolar systems. The purpose of this study is to constrain a possible range of the mass of a terrestrial planet that can get water. We focus on the process of water production through oxidation of the atmospheric hydrogen--the nebular gas having been attracted gravitationally--by oxide available at the planetary surface. For the water production to work well on a planet, a sufficient amount of hydrogen and enough high temperature to melt the planetary surface are needed. We have simulated the structure of the atmosphere that connects with the protoplanetary nebula for wide ranges of heat flux, opacity, and density of the nebular gas. We have found both requirements are fulfilled for an Earth-mass planet for wide ranges of the parameters. We have also found the surface temperature of planets of <= 0.3 Earth masses is lower than the melting temperature of silicate (~ 1500K). On the other hand, a planet of more than several Earth masses becomes a gas giant planet through runaway accretion of the nebular gas.Comment: 25 pages, 8 figures, to appear in the 01 September 2006 issue of Ap

Large-scale albuminuria screen for nephropathy models in chemically induced mouse mutants

Background/Aim: Phenotype-driven screening of a great pool of randomly mutant mice and subsequent selection of animals showing symptoms equivalent to human kidney diseases may result in the generation of novel suitable models for the study of the pathomechanisms and the identification of genes involved in kidney dysfunction. Methods: We carried out a large-scale analysis of ethylnitrosourea (ENU)-induced mouse mutants for albuminuria by using qualitative SDS-polyacrylamide gel electrophoresis. Results: The primary albuminuria screen preceded the comprehensive phenotypic mutation analysis in a part of the mice of the Munich ENU project to avoid loss of mutant animals as a consequence of prolonged suffering from severe nephropathy. The primary screen detected six confirmed phenotypic variants in 2,011 G1 animals screened for dominant mutations and no variant in 48 G3 pedigrees screened for recessive mutations. Further breeding experiments resulted in two lines showing a low phenotypic penetrance of albuminuria. The secondary albuminuria screen was carried out in mutant lines which were established in the Munich ENU project without preceding primary albuminuria analysis. Two lines showing increased plasma urea levels were chosen to clarify if severe kidney lesions are involved in the abnormal phenotype. This analysis revealed severe albuminuria in mice which are affected by a recessive mutation leading to increased plasma urea and cholesterol levels. Conclusion: Thus, the phenotypic selection of ENU-induced mutants according to the parameter proteinuria in principle demonstrates the feasibility to identify nephropathy phenotypes in ENU-mutagenized mice. Copyright (C) 2005 S. Karger AG, Basel

Detection of Escherichia coli and harmful enteric bacterial pathogens in domestic hand-dug wells in the Cuvelai Etosha Basin of Namibia

The Cuvelai Etosha Basin of Namibia is characterised by complex aquifer systems with multi-layered aquifers and various water qualities. Some parts of the basin have been covered with a pipeline system that supplies purified surface water from the Kunene River. Locations that lack a pipeline system utilisehand-dug wells as a source of drinking water. These wells draw water from shallow perched aquifers and are not protected from surface contamination nor is the water quality monitored. Sanitised water supply is relevant for the growth and development of societies and is a priority of the United Nations Millennium Development Goals. A bacteriological water quality study aimed at investigating the presence and seasonal variation of; Citrobacter, Escherichia, Klebsiella, Enterobacter, Proteus, Salmonella, Shigella, and Pseudomonasspecies was conducted on 44 hand-dug wells in the Ohangwena and Omusati regions of the Cuvelai Etosha Basin. Samples were collected from both the wet and dry seasons. Results disclosed the presence of Salmonella, Shigella, Citrobacter, Escherichia, Klebsiella, Enterobacter, Proteus, and Pseudomonas species.Chi-square confirmed a significant seasonal variation in Salmonella (P 0.05), Escherichia (P > 0.05), Klebsiella (P > 0.05), Enterobacter(P > 0.05), Proteus (P > 0.05) and Pseudomonas (P > 0.05) species.Water from these hand-dug wells is not safe for drinking unless it is subjected to appropriate treatment. It is recommended that hand-dug wells should be properly constructed at safe distances from contaminating structures such a pit latrines and routinely assessed for pathogens, and the water should be sanitized prior to consumption

Spatial analysis of fluoride concentrations in drinking water and population at risk in Namibia

© 2017, South African Water Research Commission. All rights reserved. Namibia, the driest country in sub-Saharan Africa, is largely reliant on groundwater for its potable water demand and groundwater is a major source of naturally-occurring fluoride. This study assessed the spatial distribution of fluoride in potable water and appraised the population at risk for high fluoride intake. Analysis of fluoride levels used existing databases that include 28 000 borehole locations across the country, while the population data were based on the 2011 Census. Spatial analysis and spatial statistics methods employed included Moran’s I, local indicators of spatial association (LISA), basic Euclidian distance, analysis of variance (ANOVA) and spatial overlay in a GIS environment. Fluoride concentrations above the recommended limit of 1.5 mg/L occur in a fifth of the boreholes and as much as 8% of the population across the country is at risk. Although the number of people in Namibia who are exposed to high fluoride is relatively small at a global scale, it is significant at a national level. Preventative measures against high fluoride intake are thus necessary in the country

An analysis of precipitation isotope distributions across Namibia using historical data

Global precipitation isoscapes based on the Global Network for Isotopes in Precipitation (GNIP) network are an important toolset that aid our understanding of global hydrologiccycles. Although the GNIP database is instrumental in developing global isoscapes, data coverage in some regions of hydrological interest (e.g., drylands) is low or non-existent thus the accuracy and relevance of global isoscapes to these regions is debatable. Capitalizing on existing literature isotope data, we generated rainfall isoscapes for Namibia (dryland) using the cokriging method and compared it to a globally fitted isoscape (GFI) downscaled to country level. Results showed weak correlation between observed and predicted isotope values in the GFI model (r2 < 0.20) while the cokriging isoscape showed stronger correlation (r2 = 0.67). The general trend of the local cokriging isoscape is consistent with synoptic weather systems (i.e., influences from Atlantic Ocean maritime vapour, Indian Ocean maritime vapour, Zaire Air Boundary, the Intertropical Convergence Zone and Tropical Temperate Troughs) and topography affecting the region. However, because we used the unweighted approach in this method, due to data scarcity, the absolute values could beimproved in future studies. A comparison of local meteoric water lines (LMWL) constructed from the cokriging and GFI suggested that the GFI model still reflects the global averageeven when downscaled. The cokriging LMWL was however more consistent with expectations for an arid environment. The results indicate that although not ideal, for data deficientregions such as many drylands, the unweighted cokriging approach using historical local data can be an alternative approach to modelling rainfall isoscapes that are more relevantto the local conditions compared to using downscaled global isoscapes

DPI-ELISA: a fast and versatile method to specify the binding of plant transcription factors to DNA in vitro

<p>Abstract</p> <p>Background</p> <p>About 10% of all genes in eukaryote genomes are predicted to encode transcription factors. The specific binding of transcription factors to short DNA-motifs influences the expression of neighbouring genes. However, little is known about the DNA-protein interaction itself. To date there are only a few suitable methods to characterise DNA-protein-interactions, among which the EMSA is the method most frequently used in laboratories. Besides EMSA, several protocols describe the effective use of an ELISA-based transcription factor binding assay e.g. for the analysis of human NFκB binding to specific DNA sequences.</p> <p>Results</p> <p>We provide a unified protocol for this type of ELISA analysis, termed DNA-Protein-Interaction (DPI)-ELISA. Qualitative analyses with His-epitope tagged plant transcription factors expressed in <it>E. coli </it>revealed that EMSA and DPI-ELISA result in comparable and reproducible data. The binding of <it>At</it>bZIP63 to the C-box and <it>At</it>WRKY11 to the W2-box could be reproduced and validated by both methods. We next examined the physical binding of the C-terminal DNA-binding domains of <it>At</it>WRKY33, <it>At</it>WRKY50 and <it>At</it>WRKY75 to the W2-box. Although the DNA-binding domain is highly conserved among the WRKY proteins tested, the use of the DPI-ELISA discloses differences in W2-box binding properties between these proteins. In addition to these well-studied transcription factor families, we applied our protocol to <it>At</it>BPC2, a member of the so far uncharacterised plant specific Basic Pentacysteine transcription factor family. We could demonstrate binding to GA/TC-dinucleotide repeat motifs by our DPI-ELISA protocol. Different buffers and reaction conditions were examined.</p> <p>Conclusions</p> <p>We successfully applied our DPI-ELISA protocol to investigate the DNA-binding specificities of three different classes of transcription factors from <it>Arabidopsis thaliana</it>. However, the analysis of the binding affinity of any DNA-binding protein to any given DNA sequence can be performed <it>via </it>this method. The DPI-ELISA is cost efficient, less time-consuming than other methods and provides a qualitative and quantitative readout. The presented DPI-ELISA protocol is accompanied by advice on trouble-shooting, which will enable scientists to rapidly establish this versatile and easy to use method in their laboratories.</p

An evaluation of |Vus| and precise tests of the Standard Model from world data on leptonic and semileptonic kaon decays

We present a global analysis of leptonic and semileptonic kaon decay data, including all recent results published by the BNL-E865, KLOE, KTeV, ISTRA+ and NA48 experiments. This analysis, in conjunction with precise lattice calculations of the hadronic matrix elements now available, leads to a very precise determination of |Vus| and allows us to perform several stringent tests of the Standard Model.Comment: LaTeX, 25 pages, 12 figures, 16 tables. Submitted to EPJC. v2: Minor changes for accepted version. No numerical results change

Os isotopes in SNC meteorites and their implications to the early evolution of Mars and Earth

A new development on the measurement of the Os isotopic composition by mass spectrometry using negative ions opened a new field of applications. The Re-Os systematic provides time information on the differentiation of the nobel metals. The nobel metals are strongly partitioned into metal and sulphide phases, but also the generation of silicate melts might fractionate the Re-Os system. Compared to the other isotopic systems which are mainly dating the fractionation of the alkalis and alkali-earth elements, the Re-Os system is expected to disclose entirely new information about the geochemistry. Especially the differentiation and early evolution of the planets such as the formation of the core will be elucidated with this method

Urinary eicosanoid metabolites in HIV-infected women with central obesity switching to raltegravir: an analysis from the women, integrase, and fat accumulation trial.

Chronic inflammation is a hallmark of HIV infection. Eicosanoids reflect inflammation, oxidant stress, and vascular health and vary by sex and metabolic parameters. Raltegravir (RAL) is an HIV-1 integrase inhibitor that may have limited metabolic effects. We assessed urinary F2-isoprostanes (F2-IsoPs), prostaglandin E2 (PGE-M), prostacyclin (PGI-M), and thromboxane B2 (TxB2) in HIV-infected women switching to RAL-containing antiretroviral therapy (ART). Thirty-seven women (RAL = 17; PI/NNRTI = 20) with a median age of 43 years and BMI 32 kg/m(2) completed week 24. TxB2 increased in the RAL versus PI/NNRTI arm (+0.09 versus -0.02; P = 0.06). Baseline PGI-M was lower in the RAL arm (P = 0.005); no other between-arm cross-sectional differences were observed. In the PI/NNRTI arm, 24-week visceral adipose tissue change correlated with PGI-M (rho = 0.45; P = 0.04) and TxB2 (rho = 0.44; P = 0.005) changes, with a trend seen for PGE-M (rho = 0.41; P = 0.07). In an adjusted model, age ≥ 50 years (N = 8) was associated with increased PGE-M (P = 0.04). In this randomized trial, a switch to RAL did not significantly affect urinary eicosanoids over 24 weeks. In women continuing PI/NNRTI, increased visceral adipose tissue correlated with increased PGI-M and PGE-M. Older age (≥ 50) was associated with increased PGE-M. Relationships between aging, adiposity, ART, and eicosanoids during HIV-infection require further study