79 research outputs found

    Analysis and implementation of adaptive filtered-X LMS algorithm based on reference signal self-extraction

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    By comparing conventional FXLMS (filtered-X least mean square) control algorithms, the present paper introduces an improved adaptive vibration control FXLMS algorithm based on reference signal self-extraction. It overcomes the problem of reference signal which correlated with external excitation signal is needed to be predicted in advance, namely, the reference signal is extracted from structural vibration in real time in the process of control algorithm. Its theoretical basis is: get an original vibration signal estimation using the error signal of the system and the estimation value is taken as the reference signal of adaptive filtering. In addition, to verify the feasibility and advantage of the proposed algorithm, we simulate solar panels with piezoelectric smart flexible plate and construct the corresponding experimental platform. Finally, the results presented in this paper demonstrate that the proposed algorithm is feasible, effective and achieve improvement with significantly faster convergence speed and better control effect compared with other algorithms

    Optimization and simulation of pulsed electric field treatment chamber for food sterilization

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    Abstract Pulsed Electric field (PEF) cell lysis technology, as a new technology, has been widely used in the fields of food sterilization. Nowadays, there are still several problems in PEF cell lysis: low cell lysis rate, high temperature rise, the presence of low velocity regions, etc. In this study, COMSOL Multiphysics was used to conduct simulation research, and the electric field, flow field and temperature field of plate treatment chamber, coaxial treatment chamber and co-field treatment chamber were coupled to simulate, and the advantages and disadvantages of the three classical treatment chamber structures were analyzed. In addition, this paper proposes a new structure of the co-field treatment chamber. Through the comparison and simulation of the coupling between our design and other four structures, the electric field intensity of our design treatment area is 20.03 kV/m, which is 46.2% higher than the highest of others. The temperature rise within 1s is 0.93 K, which is 2/3 lower than others. Our design almost eliminates the peak electric field area at the junction between the inside of the electrode and the insulator, and the maximum electric field intensity in this area is reduced by 24.4% compared with others

    One-pot biosynthesis of N-acetylneuraminic acid from chitin via combination of chitin-degrading enzymes, N-acetylglucosamine-2-epimerase, and N-neuraminic acid aldolase

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    N-acetylneuraminic acid (Neu5Ac) possesses the ability to promote mental health and enhance immunity and is widely used in both medicine and food fields as a supplement. Enzymatic production of Neu5Ac using N-acetyl-D-glucosamine (GlcNAc) as substrate was significant. However, the high-cost GlcNAc limited its development. In this study, an in vitro multi-enzyme catalysis was built to produce Neu5Ac using affordable chitin as substrate. Firstly, exochitinase SmChiA from Serratia proteamaculans and N-acetylglucosaminosidase CmNAGase from Chitinolyticbacter meiyuanensis SYBC-H1 were screened and combined to produce GlcNAc, effectively. Then, the chitinase was cascaded with N-acetylglucosamine-2-epimerase (AGE) and N-neuraminic acid aldolase (NanA) to produce Neu5Ac; the optimal conditions of the multi-enzyme catalysis system were 37°C and pH 8.5, the ratio of AGE to NanA (1:4) and addition of pyruvate (70 mM), respectively. Finally, 9.2 g/L Neu5Ac could be obtained from 20 g/L chitin within 24 h along with two supplementations with pyruvate. This work will lay a good foundation for the production of Neu5Ac from cheap chitin resources

    Winter pea mixtures with triticale and oat for biogas and methane production in semiarid conditions of the south Pannonian Basin

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    Due to the increase in greenhouse gases from burning fossil fuels, there is increased attention on renewable energy sources from specialized crops. These crops should not compete with food security, and it is important to select plant resources which can produce methane-rich biogas efficiently. The most commonly used energy crops are planted and managed intensively with high inputs in productive land, and this negatively affects land use and sustainable use of resources. The main purposes of this study are to: (a) determine the best cropping system for optimal biogas and methane production from sole crops of winter pea, triticale and out and their mixtures at two different maturity stages (first stage: full-flowering stage of winter pea and beginning of milky stage of cereals; second stage: emergence of firsts pods for pea and milky/waxy stage of cereals); and (b) to develop and use a surface model to determine the best combinations of various mixtures that result in highest biogas and methane. The used pure or mixtures of pea, oat and triticale in two seed weight ratios (50%:50% and 75%:25%) produced different green mass, dry matter, solids, biogas and methane yields. The experiments showed that maximum green mass was produced by the mixture of pea and oat at the seed ratio 75%:25% and when crop was harvested at the full-flowering stage of winter pea and beginning of the milky stage of cereals. After quadratic model analyses, the combination ratios of the oat and triticale were, respectively, 30% and 8%, with a maximum green biomass yield of 61.48 t ha-1, while the corresponding values were 28% and 38%, with maximum solids yields of 25.64 t ha-1. As the model was set at 100 for all three independent variables (oat, triticale and pea), the pea should be at 62% (100-30-8) and 34% (100-28-38), respectively, for green mass and organic solids yields. The results of surface analysis and multivariate analysis of variance showed that the mixture of oat and triticale had great potentiality for biogas and methane yields. The optimal mixture of oat with triticale was 27-35% with 73-65% for producing biogas and (or) methane

    Prediction of hydrocarbon source rock distribution using logging curves: A case study of Es32 source rock in Nanpu Sag, Huanghua depression, Bohai Bay Basin

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    The Es3 is the main hydrocarbon source rock system in the Nanpu Sag. Finally, the TOC and hydrocarbon potential of each sub depression of Es3were predicted. The study shows that the hydrocarbon source rocks of Es32 and Es34 sections are mainly of type II2 and type II1 respectively, with good organic matter type and high maturity. Biomarker compound parameters indicate that the Es32 section hydrocarbon source rocks developed in a semi-saline, low to medium terrestrial source organic matter supplied reduction environment with a high algal contribution; the Es34 section hydrocarbon source rocks formed in a freshwater, low terrestrial source supplied reduction environment with a medium-high algal contribution. The multiple linear regression method is more effective than the ΔlgR method in predicting hydrocarbon source rocks in the Nanpu Sag, and the prediction accuracy is higher; the correlation between TOC and S1 + S2 is the best in the model for predicting hydrocarbon potential. The TOC and hydrocarbon potential of the hydrocarbon source rocks in Es31 are generally low; the high value area of TOC and hydrocarbon potential of the hydrocarbon source rocks in Es32 is partly between the No. 1 tectonic zone and No. 5 tectonic zone in Linque sub depression, and the TOC and hydrocarbon potential of the hydrocarbon source rocks in Liunan sub depression are larger; the high value area of TOC and hydrocarbon potential of the hydrocarbon source rocks in Es34 is mainly concentrated in Shichang sub depression

    The Fungal CYP51s: Their Functions, Structures, Related Drug Resistance, and Inhibitors

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    CYP51 (Erg11) belongs to the cytochrome P450 monooxygenase (CYP) superfamily and mediates a crucial step of the synthesis of ergosterol, which is a fungal-specific sterol. It is also the target of azole drugs in clinical practice. In recent years, researches on fungal CYP51 have stepped into a new stage attributing to the discovery of crystal structures of the homologs in Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. This review summarizes the functions, structures of fungal CYP51 proteins, and the inhibitors targeting these homologs. In particular, several drug-resistant mechanisms associated with the fungal CYP51s are introduced. The sequences and crystal structures of CYP51 proteins in different fungal species are also compared. These will provide new insights for the advancement of research on antifungal agents

    Modelling the Effects of Climatic Factors on the Biomass and Rodent Distribution in a Tibetan Grassland Region in China

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    To identify the main climatic factors from 2007 to 2009 that influence biomass and rodent distribution, 576 fixed sample plots within 81 million km2 of different climatic grassland in Tibet were monitored. The aboveground biomass, the total burrows, the active burrows, the burrow index, and the rodent density in the plots were measured yearly in October. The monthly precipitation and the average temperatures from April to November were obtained for four successive years (2006-2009). Correlative and modelling analyses between the aboveground biomass, the rodent density, and the climatic factors were performed. The results showed that biomass and rodent density were significantly correlated with the climatic factors. Using ridge regression analyses, models of the biomass and rodent density with respect to the monthly precipitations and average temperatures of the previous year were developed. The raw testing data demonstrated that the models can be used approximately to predict biomass and rodent density
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