466 research outputs found
MITIGATION EFFECT OF HUMAN-COMPUTER INTERACTION ON PEOPLE WITH COGNITIVE IMPAIRMENT IN IMAGE PROCESSING
MITIGATION EFFECT OF HUMAN-COMPUTER INTERACTION ON PEOPLE WITH COGNITIVE IMPAIRMENT IN IMAGE PROCESSING
Facial Landmarks Detection and Expression Recognition in the Dark
Facial landmark detection has been widely adopted for body language analysis and facial identification task. A variety of facial landmark detectors have been proposed in different approaches, such as AAM, AdaBoost, LBF and DPM. However, most detectors were trained and tested on high resolution images with controlled environments. Recent study has focused on robust landmark detectors and obtained increasing excellent performance under different poses and light conditions. However, it remains an open question about implementing facial landmark detection in extremely dark images. Our implementation is to build an application for facial expression analysis in extremely dark environments by landmarks. To address this problem, we explored different dark image enhancement methods to facilitate landmark detection. And we designed landmark correct- ness methods to evaluate landmarks’ localization. This step guarantees the accuracy of expression recognition. Then, we analyzed the feature extraction methods, such as HOG, polar coordinate and landmarks’ distance, and normalization methods for facial expression recognition. Compared with the existing facial expression recognition system, our system is more robust in the dark environment, and performs very well in detecting happy and surprising
Crosstalk Between Cytochrome Bc1 Complex and Mitochondrial Matrix Enzymes
The interactions between the mitochondrial cytochrome bc 1 complex and matrix soluble proteins were studied by a precipitation pull-down technique. One of the matrix proteins pulled down by bc 1 complex was identified as mitochondrial malate dehydrogenase (MDH) by MALDI-TOF Mass spectrometry and confirmed by Western blotting with anti-MDH antibody. Using cross linking technique, subunits I, II (core I & II) and V of the bc 1 complex were identified as the interacting sites for MDH. Incubating purified MDH with the detergent dispersed bc 1 complex results in the increase of the activities of both the bc 1 complex and MDH. The effect of the bc 1 complex on the activities of MDH is unidirectional (oxalacetate � malate). These results suggest that the novel crosstalk between citric acid cycle enzymes and electron transfer chain complexes might play a regulatory role in mitochondrial bioenergetics. We also confirmed the existence of a short form of MDH. We found that it does not have the normal enzyme activity but may help the normal long MDH together to regulate the bioenergetics process. Another matrix protein pulled down by bc 1 complex was identified as mitochondrial aconitase. Subunit II of the bc 1 complex was identified as the interacting site for aconitase. Unlike MDH, no effect on the activity of the bc 1 complex was observed when purified aconitase was interacted with the detergent dispersed bc 1 complex. And the activity of aconitase was only enhanced slightly by cytochrome bc 1 complex. Upon the binding of aconitase, the superoxide generating activity of bc 1 complex is greatly decreased. However, when aconitase was added to the bacterial bc 1 complex, little effect on its superoxide generating activity was observed. The lack of core subunits I and II in the bacterial bc 1 complex may be the reason for this difference. The interaction between bc 1 complex and aconitase can be enhanced by calcium, which is considered as an active factor for the bioenergetic process of mitochondria. These results suggest that the binding between mitochondrial bc 1 complex and aconitase might play an important role in superoxide scavenging when mitochondria is in a high energy producing state, which is crucial for mitochondria and the whole organism. To study the co-complex of mitochondrial bc 1 complex and malate dehydrogenase (or aconitase), we developed two approaches to obtain the co-crystals. With these approaches, I got some crystals and am conducting further studies.Department of Biochemistry and Molecular Biolog
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