Application of Nursing Risk Management in Patients with Cardiovascular Emergencies

Objectives: To study the nursing effect of nursing risk management in patients with acute cardiovascular disease. Methods: A total of 62 acute and critical cardiovascular patients admitted to the Second People's Hospital of Shandong Province from November 2021 to January 2023 were selected as the study objects, and the patients were randomly divided into control group and observation group. The control group adopted conventional nursing methods, while nursing risk management measures were carried out in the observation group. The nursing effects of the two groups were compared, and the incidence rate and patient satisfaction were analyzed according to the time. Results: The effective rate of the observation group was 93.55%, significantly higher than 61.29% of the control group, and the difference was statistically significant(P<0.05). The occurrence of risk events in observation group was significantly less than that in control group, and patient satisfaction was significantly higher than that in control group (P<0.05). Conclusion: Nursing risk management could significantly improve the nursing effect of patients, reduce the occurrence of risk events, improve patient satisfaction, has a certain application value

Development of FRET-based high-throughput screening for viral RNase III inhibitors

The class 1 ribonuclease III (RNase III) encoded by Sweet potato chlorotic stunt virus (CSR3) suppresses RNA silencing in plant cells and thereby counters the host antiviral response by cleaving host small interfering RNAs, which are indispensable components of the plant RNA interference (RNAi) pathway. The synergy between sweet potato chlorotic stunt virus and sweet potato feathery mottle virus can reduce crop yields by 90%. Inhibitors of CSR3 might prove efficacious to counter this viral threat, yet no screen has been carried out to identify such inhibitors. Here, we report a novel high-throughput screening (HTS) assay based on fluorescence resonance energy transfer (FRET) for identifying inhibitors of CSR3. For monitoring CSR3 activity via HTS, we used a small interfering RNA substrate that was labelled with a FRET-compatible dye. The optimized HTS assay yielded 109 potential inhibitors of CSR3 out of 6,620 compounds tested from different small-molecule libraries. The three best inhibitor candidates were validated with a dose-response assay. In addition, a parallel screen of the selected candidates was carried out for a similar class 1 RNase III enzyme from Escherichia coli (EcR3), and this screen yielded a different set of inhibitors. Thus, our results show that the CSR3 and EcR3 enzymes were inhibited by distinct types of molecules, indicating that this HTS assay could be widely applied in drug discovery of class 1 RNase III enzymes.Peer reviewe

Aging agricultural labor force, outsourcing service of pest control and biopesticide application: a case study of 10 counties in Fujian Province

IntroductionUnder the background of increasingly severe aging of agricultural labor force and relatively slow land circulation, agricultural production outsourcing has become the main means of agricultural modernization. Despite the existence of a voluminous literature on outsourcing service of pest control, little is known about its effect on biopesticides use.MethodsTo fill this knowledge gap, this study uses rice planting data from 1,045 Chinese farm households to analyze the impact of different aging agricultural households and the pest control outsourcing service on the application of biopesticides by using the logit regression model.Results and DiscussionThe study results showed that: (1) Participation in pest control outsourcing services significantly promotes biopesticide application. But this positive effect varied across age groups. (2) Compared with aging households, non-aging farmers tend to use biopesticides. Given this, it is recommended that pay attention to the ecological effect of pest control outsourcing services, strengthen the assistance to the aging labor group in the green pesticide promotion policy, and guide the aged planting decision makers to participate in pest control outsourcing services, thereby effectively improving the sustainable development of green agriculture

A fast-multi-pole accelerated method of fundamental solutions for 2-D broadband scattering of SH waves in an infinite half space

The traditional method of fundamental solution (T-MFS) is known as an effective method for solving the scattering of elastic waves, but the T-MFS is inefficient in solving large-scale or broadband frequency problems. Therefore, in order to improve the performance in efficiency and memory requirement for treating practical complex 2-D broadband scattering problems, a new algorithm of fast multi-pole accelerated method of fundamental solution (FM-MFS) is proposed. Taking the 2-D scattering of SH waves around irregular scatterers in an elastic half-space as an example, the implementation steps are presented in detail. Based on the accuracy and efficiency verification, the FM-MFS is applied to solve the broadband frequency scattering of plane SH waves around group cavities, inclusions, a V-shaped canyon and a semi-elliptical hill. It shows that, compared with T-MFS, the FM-MFS has great advantages in reducing the consumed CPU time and memory for 2-D broadband scattering. Besides, the FM-MFS has excellent adaptability both for broad-frequency and complex-shaped scattering problems

Phenotyping viral infection in sweetpotato using a high-throughput chlorophyll fluorescence and thermal imaging platform

Background Virus diseases caused by co-infection with Sweet potato feathery mottle virus (SPFMV) and Sweetpotato chlorotic stunt virus (SPCSV) are a severe problem in the production of sweetpotato (Ipomoea batatas L.). Traditional molecular virus detection methods include nucleic acid-based and serological tests. In this study, we aimed to validate the use of a non-destructive imaging-based plant phenotype platform to study plant-virus synergism in sweetpotato by comparing four virus treatments with two healthy controls. Results By monitoring physiological and morphological effects of viral infection in sweetpotato over 29 days, we quantified photosynthetic performance from chlorophyll fluorescence (ChlF) imaging and leaf thermography from thermal infrared (TIR) imaging among sweetpotatoes. Moreover, the differences among different treatments observed from ChlF and TIR imaging were related to virus accumulation and distribution in sweetpotato. These findings were further validated at the molecular level by related gene expression in both photosynthesis and carbon fixation pathways. Conclusion Our study validated for the first time the use of ChlF- and TIR-based imaging systems to distinguish the severity of virus diseases related to SPFMV and SPCSV in sweetpotato. In addition, we demonstrated that the operating efficiency of PSII and photochemical quenching were the most sensitive parameters for the quantification of virus effects compared with maximum quantum efficiency, non-photochemical quenching, and leaf temperature.Peer reviewe

In Vitro Identification and In Vivo Confirmation of Inhibitors for Sweet Potato Chlorotic Stunt Virus RNA Silencing Suppressor, a Viral RNase III

Sweet potato virus disease (SPVD), caused by synergistic infection of Sweet potato chlorotic stunt virus (SPCSV) and Sweet potato feathery mottle virus (SPFMV), is responsible for substantial yield losses all over the world. However, there are currently no approved treatments for this severe disease. The crucial role played by RNase III of SPCSV (CSR3) as an RNA silencing suppressor during the viruses' synergistic interaction in sweetpotato makes it an ideal drug target for developing antiviral treatment. In this study, high-throughput screening (HTS) of small molecular libraries targeting CSR3 was initiated by a virtual screen using Glide docking, allowing the selection of 6,400 compounds out of 136,353. We subsequently developed and carried out kinetic-based HTS using fluorescence resonance energy transfer technology, which isolated 112 compounds. These compounds were validated with dose-response assays including kinetic-based HTS and binding affinity assays using surface plasmon resonance and microscale thermophoresis. Finally, the interference of the selected compounds with viral accumulation was verified in planta. In summary, we identified five compounds belonging to two structural classes that inhibited CSR3 activity and reduced viral accumulation in plants. These results provide the foundation for developing antiviral agents targeting CSR3 to provide new strategies for controlling sweetpotato virus diseases. IMPORTANCE We report here a high-throughput inhibitor identification method that targets a severe sweetpotato virus disease caused by coinfection with two viruses (SPCSV and SPFMV). The disease is responsible for up to 90% yield losses. Specifically, we targeted the RNase III enzyme encoded by SPCSV, which plays an important role in suppressing the RNA silencing defense system of sweetpotato plants. Based on virtual screening, laboratory assays, and confirmation in planta, we identified five compounds that could be used to develop antiviral drugs to combat the most severe sweetpotato virus disease.Peer reviewe