30 research outputs found
Theory and applications of atomic and ionic polarizabilities
Atomic polarization phenomena impinge upon a number of areas and processes in
physics. The dielectric constant and refractive index of any gas are examples
of macroscopic properties that are largely determined by the dipole
polarizability. When it comes to microscopic phenomena, the existence of
alkaline-earth anions and the recently discovered ability of positrons to bind
to many atoms are predominantly due to the polarization interaction. An
imperfect knowledge of atomic polarizabilities is presently looming as the
largest source of uncertainty in the new generation of optical frequency
standards. Accurate polarizabilities for the group I and II atoms and ions of
the periodic table have recently become available by a variety of techniques.
These include refined many-body perturbation theory and coupled-cluster
calculations sometimes combined with precise experimental data for selected
transitions, microwave spectroscopy of Rydberg atoms and ions, refractive index
measurements in microwave cavities, ab initio calculations of atomic structures
using explicitly correlated wave functions, interferometry with atom beams, and
velocity changes of laser cooled atoms induced by an electric field. This
review examines existing theoretical methods of determining atomic and ionic
polarizabilities, and discusses their relevance to various applications with
particular emphasis on cold-atom physics and the metrology of atomic frequency
standards.Comment: Review paper, 44 page
MT1-MMP regulates urothelial cell invasion via transcriptional regulation of Dickkopf-3
Membrane type-1 matrix metalloproteinase (MT1-MMP) is a zinc-binding endopeptidase, which plays a crucial role in tumour growth, invasion and metastasis. We have shown previously that MT1-MMP has higher expression levels in the human urothelial cell carcinoma (UCC) tissue. We show here that siRNA against MT1-MMP blocks invasion in UCC cell lines. Invasion is also blocked by broad-spectrum protease and MMP inhibitors including tissue inhibitor of metalloproteinase-1 and -2. Membrane type-1-MMP can also regulate transcription. We have used expression arrays to identify genes that are differentially transcribed when siRNA is used to suppress MT1-MMP expression. Upon MT1-MMP knockdown, Dickkopf-3 (DKK3) expression was highly upregulated. The stability of DKK3 mRNA was unaffected under these conditions, suggesting transcriptional regulation of DKK3 by MT1-MMP. Dickkopf-3 has been previously shown to inhibit invasion. We confirm that the overexpression of DKK3 leads to decreased invasive potential as well as delayed wound healing. We show for the first time that the effects of MT1-MMP on cell invasion are mediated in part through changes in DKK3 gene transcription
MMP28 (epilysin) as a novel promoter of invasion and metastasis in gastric cancer
Background\ud
The purpose of this study was to investigate invasion and metastasis related genes in gastric cancer.\ud
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Methods\ud
The transwell migration assay was used to select a highly invasive sub-line from minimally invasive parent gastric cancer cells, and gene expression was compared using a microarray. MMP28 upregulation was confirmed using qRT-PCR. MMP28 immunohistochemistry was performed in normal and gastric cancer specimens. Invasiveness and tumor formation of stable cells overexpressing MMP28 were tested in vitro and in vivo.\ud
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Results\ud
MMP28 was overexpressed in the highly invasive sub-cell line. Immunohistochemistry revealed MMP28 expression was markedly increased in gastric carcinoma relative to normal epithelia, and was significantly associated with depth of tumor invasion, lymph node metastasis and poorer overall survival. Ectopic expression of MMP28 indicated MMP28 promoted tumor cell invasion in vitro and increased gastric carcinoma metastasis in vivo.\ud
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Conclusions\ud
This study indicates MMP28 is frequently overexpressed during progression of gastric carcinoma, and contributes to tumor cell invasion and metastasis. MMP28 may be a novel therapeutic target for prevention and treatment of metastases in gastric cancer