15 research outputs found

    Exploring natural resources for sustainable management of ecosystems future challenges for control and management of Xyleborus fornicatus eichh. (Coleoptera Scolytidae), the shot-hole borer of tea in Sri Lanka

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    In view of the highly diverse genetic base of the seedling tea plants, though cultivated as a monocrop,along with shade trees and surrounding forests, provided a reasonably stable ecosystem then. Withthe introduction of high yielding vegetatively propagated tea during 1950's, a significant change in thedistribution and population densities of pests has been taken place. Shot-hole borer is one such pestand since then it has become the most serious and damaging pest of tea in Sri Lanka. Control hasbeen a difficult task as a result of its' wide distribution from near sea level up to 1500m amsl and theconcealed habit virtually protected from parasites and predators. Biological control usingentomopathogenic fungus, Beauvaria bassiana Vuillemin (Balsomo ) is being viewed as anenvironmentally friendly alternative to chemical control in the light of growing concern on the usage of pesticides and since oflate, the detection of pesticide residues in made tea. Preliminary investigationswere carried out with a view to find out a suitable local strain/s of the fungus for use against this pest.Laboratory studies have shown that strains of this fungus isolated from a tea garden in Talawakelle(Nuwera Eliya District) and a home garden in Welimada (Badulla District) are highly pathogenic toshot-hole borer imparting more than 90% mortality. A potential exists for using the locally availablenatural resources like entomopathogenic fungi for the management of key pests in a compatible andecologically acceptable manner. This forms the basis for Integrated Pest Management (lPM) approachof key pests. These efforts will promote and ensure the sustainable development of the tea ccosyst;m

    Antibacterial activities of endophytic fungi isolated from six Sri Lankan plants of the family Cyperaceae

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    In a study designed to determine the antibacterial potential of endophytic fungi inhabiting plants of Cyperaceae family, 72 morphologically distinct endophytic fungi were isolated from six plants and their antibacterial activities against two Gram positive and three Gram negative pathogenic bacterial species were examined. Two antibacterial metabolites from two endophytes were also isolated and their chemical structures and minimum inhibitory concentrations were determined. Sixty six fungal extracts (92%) were active against at least one bacterium tested. Butyrolactone I isolated from the Aspergillus terreus and 9-epi viridol isolated from Trichoderma virens exhibited MIC values in the range 128-256 µg/mL against Gram positive Bacillus subtilits, Staphylococcus aureus, methicillin resistant S. aureus (MRSA) and Escherichia coli. Both compounds were inactive against other micro-organisms tested. This study confirmed that Cyperaceae plants harbor numerous endophytes that produce antibacterial metabolites active against both Gram positive and, to a lesser extent, Gram negative bacteria. Video Clip of Methodology: Column Chromatogrpahy: 15 min 34 sec   Full Screen   Alternat
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