20 research outputs found
Phosphatase activity in sandy soil influenced by mycorrhizal and non-mycorrhizal cover crops
Recovery of soil microbiological properties in a degraded area planted with Corymbia citriodora and Leucaena leucocephala
Características biológicas do solo indicadoras de qualidade após dois anos de aplicação de biossólido industrial e cultivo de milho
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Metagenomic analysis of permafrost microbial community response to thaw
We employed deep metagenomic sequencing to determine the impact of thaw on microbial phylogenetic and functional genes and related this data to measurements of methane emissions. Metagenomics, the direct sequencing of DNA from the environment, allows for the examination of whole biochemical pathways and associated processes, as opposed to individual pieces of the metabolic puzzle. Our metagenome analyses revealed that during transition from a frozen to a thawed state there were rapid shifts in many microbial, phylogenetic and functional gene abundances and pathways. After one week of incubation at 5°C, permafrost metagenomes converged to be more similar to each other than while they were frozen. We found that multiple genes involved in cycling of C and nitrogen shifted rapidly during thaw. We also constructed the first draft genome from a complex soil metagenome, which corresponded to a novel methanogen. Methane previously accumulated in permafrost was released during thaw and subsequently consumed by methanotrophic bacteria. Together these data point towards the importance of rapid cycling of methane and nitrogen in thawing permafrost