1 research outputs found
Role of the Rosa canina L. leaf extract as an antidiarrheal drug in rodents
Objectives: The objective of the study was to investigate the effect of
the leaf extract of Rosa canina L. against experimental diarrhea
induced by castor oil in rodents. Materials and Methods: The methanol
extract of Rosa canina L. (30 and 60 mg/kg body weight) was
administered orally to two groups of mice (five animals per group) in
order to evaluate the activity of the extract against the castor
oil-induced diarrhea model in mice. Two other groups received normal
saline and diphenoxylate (5 mg/kg) as positive control. The effect of
the extract on intestinal transit and castor oil-induced intestinal
fluid accumulation (enteropooling) was assessed. The effects of the
extract on the isolated rabbit jejunum and on the isolated rat ileum
were studied. Results: The preliminary phytochemical screening of the
leaf extract of Rosa Canina L. revealed the presence of alkaloids,
flavonoids, glycosides, saponins, and volatile oil. Intraperitoneal
LD50 of the extract was found to be 455.19 ± 23 mg/kg in mice. The
antidiarrheal effect of the methanolic extract exhibited a
concentration-dependent inhibition of the spontaneous pendular movement
of the isolated rabbit jejunum and inhibited acetylcholine-induced
contraction of the rat ileum. A dose-dependent decrease in
gastrointestinal transit was observed with extracts (30 and 60 mg/kg),
which also protected mice against castor oil-induced diarrhea and
castor oil-induced fluid accumulation, respectively. Conclusions: The
presence of some of the phytochemicals in the leaf extract may be
responsible for the observed effects, and also the basis for its use in
traditional medicine as an antidiarrheal drug