Research on Multi-leg Inventory Control Based on Passenger Choice

To remedy the lack of mathematical programming and the Expected Marginal Seat Revenue (EMSR) model for multi-leg seat inventory control, this paper proposes a method based on passenger choice. Except for data about which seats passengers decide to opt for, there is no need to obtain distributions of passengers’ demands or other “a priori” information. The proposed method can discover the real factors that affect passengers’ choices, and then estimate the probabilities of seat choices and the revenue according to the weights of the factors. Simulated experiments and comparison with the shadow price method and the virtual “bucket” method confirm the feasibility and effectiveness of the proposed method in seat inventory control for multi-leg flights

An ant colony optimization approach for maximizing the lifetime of heterogeneous wireless sensor networks

Maximizing the lifetime of wireless sensor networks (WSNs) is a challenging problem. Although some methods exist to address the problem in homogeneous WSNs, research on this problem in heterogeneous WSNs have progressed at a slow pace. Inspired by the promising performance of ant colony optimization (ACO) to solve combinatorial problems, this paper proposes an ACO-based approach that can maximize the lifetime of heterogeneous WSNs. The methodology is based on finding the maximum number of disjoint connected covers that satisfy both sensing coverage and network connectivity. A construction graph is designed with each vertex denoting the assignment of a device in a subset. Based on pheromone and heuristic information, the ants seek an optimal path on the construction graph to maximize the number of connected covers. The pheromone serves as a metaphor for the search experiences in building connected covers. The heuristic information is used to reflect the desirability of device assignments. A local search procedure is designed to further improve the search efficiency. The proposed approach has been applied to a variety of heterogeneous WSNs. The results show that the approach is effective and efficient in finding high-quality solutions for maximizing the lifetime of heterogeneous WSNs

An efficient multi-attribute multi-item auction mechanism with ex-ante and ex-post satisfaction for 4PL transportation service procurement

Reverse auction is an effective tool for a 4PL to purchase transportation services. This paper investigated a new transportation services procurement problem for 4PL, which involves three features: the 4PL’s loss-averse behavior, price and non-price attributes, and multiple transportation requests. An efficient multi-attribute multi-item reverse auction mechanism considering the 4PL ex-ante and ex-post satisfaction (EES-MMRA) is proposed to purchase transportation services for the 4PL. In the EES-MMRA, integrating the allocation rule with the 4PL ex-ante satisfaction, a 0-1 programming model is constructed to determine winning 3PLs and obtain efficient allocations. Then, a payment rule considering the 4PL ex-post satisfaction is established to ensure truthful bidding of 3PLs. And we discuss some desirable properties (e.g., incentive compatibility, individual rationality, efficiency, and budget balance properties) to justify the EES-MMRA mechanism, subsequently. Next, several numerical experiments are conducted to demonstrate the effectiveness and applicability of the EES-MMRA mechanism. Furthermore, sensitivity analysis presents the influences of the weights of the non-price attributes, risk attitude coefficients, and loss aversion coefficients. Finally, we conduct comparison analysis to show the advantages of the EES-MMRA mechanism over the known Vickrey–Clark–Groves (P-VCG) mechanism

Medium-term Outcomes of Myocarditis and Pericarditis following BNT162b2 Vaccination among Adolescents in Hong Kong

In this study, we examined the clinical and electrophysiological outcomes of adolescents in Hong Kong who developed myocarditis or pericarditis following BNT162b2 vaccination for COVID-19, and followed-up for 60 to 180 days after their initial diagnosis. Clinical assessments included electrocardiogram (ECG) and echocardiogram at the initial admission and follow-up were compared. Treadmill testing was also performed in some cases. Between 14 June 2021 and 16 February 2022, 53 subjects were approached to participate in this follow-up study, of which 28 patients were followed up for >60 days with a median follow-up period of 100 days (range, 61-178 days) and were included in this study. On admission, 23 patients had ECG abnormalities but no high-grade atrioventricular block. Six patients had echocardiogram abnormalities, including reduced contractility, small rim pericardial effusions, and hyperechoic ventricular walls. All patients achieved complete recovery on follow-up. After discharge, 10 patients (35.7%) reported symptoms, including occasional chest pain, shortness of breath, reduced exercise tolerance, and recurrent vasovagal near-syncope. At follow-up, assessments, including ECGs, were almost all normal. Among the three patients with possible ECG abnormalities, all their echocardiograms or treadmill testings were normal. Sixteen patients (57.1%) underwent treadmill testing at a median of 117 days post-admission, which were also normal. However, at follow-up, there was a significant mean bodyweight increase of 1.81kg (95%CI 0.47-3.1 kg, p=0.01), possibly due to exercise restriction. In conclusion, most adolescents experiencing myocarditis and pericarditis following BNT162b2 vaccination achieved complete recovery. Some patients developed non-specific persistent symptoms, and bodyweight changes shall be monitored

Efficacy, safety and immunogenicity of a human rotavirus vaccine (RIX4414) in Hong Kong children up to three years of age: A randomized, controlled trial

AbstractBackgroundA phase III, double-blind, randomized, controlled trial was conducted in Hong Kong to evaluate the efficacy, safety and immunogenicity of a human rotavirus vaccine, RIX4414 (Rotarix™) against severe rotavirus gastroenteritis in children up to three years of age.MethodsHealthy infants aged 6–12 weeks were enrolled between 08-December-2003 and 31-August-2005 and received two oral doses of either RIX4414 vaccine (N=1513) or placebo (N=1512) given 2 months apart. Vaccine efficacy was assessed from two weeks post-Dose 2 until the children were two and three years of age. Anti-rotavirus IgA seroconversion rate was calculated pre-vaccination and 1–2 months post-Dose 2 using ELISA (cut-off=20U/mL) for 100 infants. Safety was assessed until the children were two years of age; serious adverse events (SAEs) were recorded throughout the study period.ResultsIn children aged two and three years of life, vaccine efficacy against severe rotavirus gastroenteritis was 95.6% (95% CI: 73.1%–99.9%) and 96.1% (95% CI: 76.5%–99.9%), respectively. The seroconversion rate 1–2 months after the second dose of RIX4414 was 97.5% (95% CI: 86.8%–99.9%). At least one SAE was recorded in 439 and 477 infants who were administered RIX4414 and placebo, respectively (p-value=0.130). Six intussusception cases were reported (RIX4414=4; placebo=2) and none was assessed to be vaccine-related.ConclusionRIX4414 was efficacious, immunogenic and safe in the prevention of rotavirus gastroenteritis for at least two years post-vaccination in Hong Kong children

A Large Population Histology Study Showing the Lack of Association between ALT Elevation and Significant Fibrosis in Chronic Hepatitis B

OBJECTIVE: We determined the association between various clinical parameters and significant liver injury in both hepatitis B e antigen (HBeAg)-positive and HBeAg-negative patients. METHODS: From 1994 to 2008, liver biopsy was performed on 319 treatment-naive CHB patients. Histologic assessment was based on the Knodell histologic activity index for necroinflammation and the Ishak fibrosis staging for fibrosis. RESULTS: 211 HBeAg-positive and 108 HBeAg-negative patients were recruited, with a median age of 31 and 46 years respectively. 9 out of 40 (22.5%) HBeAg-positive patients with normal ALT had significant histologic abnormalities (necroinflammation grading >/= 7 or fibrosis score >/= 3). There was a significant difference in fibrosis scores among HBeAg-positive patients with an ALT level within the Prati criteria (30 U/L for men, 19 U/L for women) and patients with a normal ALT but exceeding the Prati criteria (p = 0.024). Age, aspartate aminotransferase and platelet count were independent predictors of significant fibrosis in HBeAg-positive patients with an elevated ALT by multivariate analysis (p = 0.007, 0.047 and 0.045 respectively). HBV DNA and platelet count were predictors of significant fibrosis in HBeAg-negative disease (p = 0.020 and 0.015 respectively). An elevated ALT was not predictive of significant fibrosis for HBeAg-positive (p = 0.345) and -negative (p = 0.544) disease. There was no significant difference in fibrosis staging among ALT 1-2 x upper limit of normal (ULN) and > x 2 ULN for both HBeAg-positive (p = 0.098) and -negative (p = 0.838) disease. CONCLUSION: An elevated ALT does not accurately predict significant liver injury. Decisions on commencing antiviral therapy should not be heavily based on a particular ALT threshold.published_or_final_versio

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data