TECHE Lab Logo Design

In 2020, Cal Poly’s Biomedical Engineering Department changed the name of one of their existing labs, the QPLUS lab, to the TECHE lab. TECHE stands for Transforming Engineers Through Hands-On Engagement and is a lab where biomedical engineering students can work together on projects, prototype ideas, and create solutions that help improve the lives of those who have disabilities. Since renaming the lab 2 years ago, the biomedical engineering department has not done any other updates to the branding of the TECHE lab and does not currently have a logo to represent the lab. The TECHE lab is a large reason why many students say yes to coming to Cal Poly and being a part of the biomedical engineering department. This is why it is so important to create a logo and branding that represents the lab and its importance to Cal Poly. I will be working with the labs\u27 director and department chair of the biomedical engineering department to create a logo for the TECHE lab. This logo can be used for all sorts of things such as a largescale window design, letterheads that will be sent to possible donors, and t-shirts. The success of this project will be measured by the approval of the logo and branding from the labs\u27 director, who is also the department chair of the biomedical engineering department

Distant horizontal gene transfer is rare for multiple families of prokaryotic insertion sequences

Horizontal gene transfer in prokaryotes is rampant on short and intermediate evolutionary time scales. It poses a fundamental problem to our ability to reconstruct the evolutionary tree of life. Is it also frequent over long evolutionary distances? To address this question, we analyzed the evolution of 2,091 insertion sequences from all 20 major families in 438 completely sequenced prokaryotic genomes. Specifically, we mapped insertion sequence occurrence on a 16S rDNA tree of the genomes we analyzed, and we also constructed phylogenetic trees of the insertion sequence transposase coding sequences. We found only 30 cases of likely horizontal transfer among distantly related prokaryotic clades. Most of these horizontal transfer events are ancient. Only seven events are recent. Almost all of these transfer events occur between pairs of human pathogens or commensals. If true also for other, non-mobile DNA, the rarity of distant horizontal transfer increases the odds of reliable phylogenetic inference from sequence dat

Evolutionary dynamics of the LTR retrotransposons roo and rooA inferred from twelve complete Drosophila genomes

BACKGROUND: Roo is the most abundant retrotransposon in the fruit fly Drosophila melanogaster. Its evolutionary origins and dynamics are thus of special interest for understanding the evolutionary history of Drosophila genome organization. We here study the phylogenetic distribution and evolution of roo, and its highly diverged relative rooA in 12 completely sequenced genomes of the genus Drosophila. RESULTS: We identify a total of 164 roo copies, 57 of which were previously unidentified copies that occur in 9 of the 12 genomes. Additionally we find 66 rooA copies in four genomes and remnants of this element in two additional genomes. We further increased the number of elements by searching for individual roo/rooA sequence domains. Most of our roo and rooA elements have been recently inserted. Most elements within a genome are highly similar. A comparison of the phylogenetic tree of our roo and rooA elements shows that the split between roo and rooA took place early in Drosophila evolution. Furthermore there is one incongruency between the species tree and the phylogenetic tree of the roo element. This incongruency regards the placement of elements from D. mojavensis, which are more closely related to D. melanogaster than elements from D. willistoni. CONCLUSION: Within genomes, the evolutionary dynamics of roo and rooA range from recent transpositional activity to slow decay and extinction. Among genomes, the balance of phylogenetic evidence, sequence divergence distribution, and the occurrence of solo-LTR elements suggests an origin of roo/rooA within the Drosophila clade. We discuss the possibility of a horizontal gene transfer of roo within this clade

Isolation and purification of mitochondria

Isolation and purification of mitochondri

Exploring the black box of quality improvement collaboratives: modelling relations between conditions, applied changes and outcomes

<p>Abstract</p> <p>Introduction</p> <p>Despite the popularity of quality improvement collaboratives (QICs) in different healthcare settings, relatively little is known about the implementation process. The objective of the current study is to learn more about relations between relevant conditions for successful implementation of QICs, applied changes, perceived successes, and actual outcomes.</p> <p>Methods</p> <p>Twenty-four Dutch hospitals participated in a dissemination programme based on QICs. A questionnaire was sent to 237 leaders of teams who joined 18 different QICs to measure changes in working methods and activities, overall perceived success, team organisation, and supportive conditions. Actual outcomes were extracted from a database with team performance indicator data. Multi-level analyses were conducted to test a number of hypothesised relations within the cross-classified hierarchical structure in which teams are nested within QICs and hospitals.</p> <p>Results</p> <p>Organisational and external change agent support is related positively to the number of changed working methods and activities that, if increased, lead to higher perceived success and indicator outcomes scores. Direct and indirect positive relations between conditions and perceived success could be confirmed. Relations between conditions and actual outcomes are weak. Multi-level analyses reveal significant differences in organisational support between hospitals. The relation between perceived successes and actual outcomes is present at QIC level but not at team level.</p> <p>Discussion</p> <p>Several of the expected relations between conditions, applied changes and outcomes, and perceived successes could be verified. However, because QICs vary in topic, approach, complexity, and promised advantages, further research is required: first, to understand why some QIC innovations fit better within the context of the units where they are implemented; second, to assess the influence of perceived success and actual outcomes on the further dissemination of projects over new patient groups.</p

The predominantly selfing plant Arabidopsis thaliana experienced a recent reduction in transposable element abundance compared to its outcrossing relative Arabidopsis lyrata

Background - Transposable elements (TEs) are major contributors to genome evolution. One factor that influences their evolutionary dynamics is whether their host reproduces through selfing or through outcrossing. According to the recombinational spreading hypothesis, for instance, TEs can spread more easily in outcrossing species through recombination, and should thus be less abundant in selfing species. We here studied the distribution and evolutionary dynamics of TE families in the predominantly selfing plant Arabidopsis thaliana and its close outcrossing relative Arabidopsis lyrata on a genome-wide scale. We characterized differences in TE abundance between them and asked which, if any, existing hypotheses about TE abundances may explain these differences. Results - We identified 1,819 TE families representing all known classes of TEs in both species, and found three times more copies in the outcrossing A. lyrata than in the predominantly selfing A. thaliana, as well as ten times more TE families unique to A. lyrata. On average, elements in A. lyrata are younger than elements in A. thaliana. In particular, A. thaliana shows a marked decrease in element number that occurred during the most recent 10% of the time interval since A. thaliana split from A. lyrata. This most recent period in the evolution of A. thaliana started approximately 500,000 years ago, assuming a splitting time of 5 million years ago, and coincides with the time at which predominant selfing originated. Conclusions - Our results indicate that the mating system may be important for determining TE copy number, and that selfing species are likely to have fewer TEs

Constraints on B and Higgs Physics in Minimal Low Energy Supersymmetric Models

We study the implications of minimal flavor violating low energy supersymmetry scenarios for the search of new physics in the B and Higgs sectors at the Tevatron collider and the LHC. We show that the already stringent Tevatron bound on the decay rate B_s -> mu+ mu- sets strong constraints on the possibility of generating large corrections to the mass difference Delta M_s of the B_s eigenstates. We also show that the B_s -> mu+ mu- bound together with the constraint on the branching ratio of the rare decay b -> s gamma has strong implications for the search of light, non-standard Higgs bosons at hadron colliders. In doing this, we demonstrate that the former expressions derived for the analysis of the double penguin contributions in the Kaon sector need to be corrected by additional terms for a realistic analysis of these effects. We also study a specific non-minimal flavor violating scenario, where there are flavor changing gluino-squark-quark interactions, governed by the CKM matrix elements, and show that the B and Higgs physics constraints are similar to the ones in the minimal flavor violating case. Finally we show that, in scenarios like electroweak baryogenesis which have light stops and charginos, there may be enhanced effects on the B and K mixing parameters, without any significant effect on the rate of B_s -> mu+ mu-.Comment: 40 pages, 14 figures; added references and note about recent measurement

A Wearable Fall Detection System Based on Body Area Networks

Falls can have serious consequences for people, leading to restrictions in mobility or, in the worst case, to traumatic-based cases of death. To provide rapid assistance, a portable fall detection system has been developed that is capable of detecting fall situations and, if necessary, alerting emergency services without any user interaction. The prototype is designed to facilitate reliable fall detection and to classify several fall types and human activities. This solution represents a life-saving service for every person that will significantly improve assistance in the case of fall events, which are a part of daily life. Additionally, this approach facilitates independent system operation, since the system does not depend on sensor or network units located within a building structure. This article also introduces fall analysis. To guarantee functional safety, a hazard analysis method named system-theoretic accident model and processes (STAMP) is applied

Quantum metastability in time-periodic potentials

In this paper we investigate quantum metastability of a particle trapped in between an infinite wall and a square barrier, with either a time-periodically oscillating barrier (Model A) or bottom of the well (Model B). Based on the Floquet theory, we derive in each case an equation which determines the stability of the metastable system. We study the influence on the stability of two Floquet states when their Floquet energies (real part) encounter a direct or an avoided crossing at resonance. The effect of the amplitude of oscillation on the nature of crossing of Floquet energies is also discussed. It is found that by adiabatically changing the frequency and amplitude of the oscillation field, one can manipulate the stability of states in the well. By means of a discrete transform, the two models are shown to have exactly the same Floquet energy spectrum at the same oscillating amplitude and frequency. The equivalence of the models is also demonstrated by means of the principle of gauge invariance.Comment: 15 pages, 7 figure

Development of an Advanced 3D Cone Beam Tomographic System

While 2D x-ray CT is now commonly used for NDT applications, the interest in 3D x-ray cone beam tomography has significantly increased over the last few years [1,2]. The need to reduce acquisition time for industrial applications, or radiation dose to patients for medical applications, led engineers to develop a new type of tomograph using x-ray beams more efficiently. Thanks to a high spatial resolution, the 3D x-ray CT allows greater knowledge of the object structure. This understanding is necessary to study and improve industrial production of high technology materials. Therefore, 3D x-ray CT is well suited for the study of materials with structural anomalies, such as technical ceramics [2]