Toward a Deeper Understanding of the Mechanisms of Animal-Assisted Interventions: How Important is the Animal?

There is an ever-increasing interest in animal-assisted interventions, and while its effects seem promising, little is known about the underlying mechanisms. The literature on animal-assisted interventions generally assumes that the animal itself is responsible for the effects of the interventions. However, evidence from placebo research suggests that a significant portion of treatment effects can be explained by contextual factors that are not specific to a treatment itself. Regarding animal-assisted interventions, this would suggest that the effects are not due to the animal but to contextual factors. In order to better understand the role of the animal and contextual factors in animal-assisted interventions, this thesis pursued two aims. First, it investigated to what extent the effects of animal-assisted interventions on pain can be attributed to the presence of an animal or to how the animal is embedded in the treatment rationale. Second, it identified the hypotheses previous studies have pursued regarding the underlying mechanisms of animal-assisted interventions and what factors have been considered as specific and nonspecific. Two different approaches were applied to address these two aims. For the first aim, we conducted two randomized controlled trials with healthy participants in a heat-pain placebo paradigm (Study I and Study II). For the second aim, a systematic review was conducted to assess factor hypotheses that researchers have presented in previous studies on animal-assisted interventions and to identify what specific and nonspecific factors have been considered in animal- assisted interventions (Study III). In the two experimental heat-pain studies, we did not find any analgesic effects in healthy participants compared to the control group when the dog was not part of the treatment rationale (Study I). Instead, participants experienced heat-pain to be more intense at the limit of their tolerance in the presence of the dog compared to the control group (i.e., self-reported pain intensity at the limit of pain tolerance, p = 0.041). When the dog was part of the treatment rationale (Study II), it did have a positive effect on pain perception in healthy participants compared to the control group (i.e., self-reported ratings of pain unpleasantness at the limit of pain tolerance, p = 0.010). The systematic review (Study III) found that a majority of studies did not define specific hypotheses regarding potential mechanisms of animal-assisted interventions. Further, most studies controlled for the animal or the interaction with the animal as specific factors. Based on the findings of this thesis, it is urgent to reconsider the explanatory model for the effectiveness of animal-assisted interventions. More precisely, instead of only focusing on the animal in animal-assisted interventions, researchers and practitioners should start to include contextual factors in their explanatory models. A better understanding of the relevant factors in animal-assisted interventions might also reveal how important the animal is and whether these effects can be facilitated through other factors

Lack of effects of the presence of a dog on pain perception in healthy participants - a randomized controlled trial

Animal-assisted interventions (AAIs) have been shown to be effective in the treatment of pain. Studies suggest that relationships with animals can have comparable qualities to relationships with humans and that this enables animals to provide social support. Further, the presence of an animal can strengthen the therapeutic alliance between patients and treatment providers. This suggests that the analgesic effects of AAI might be mediated by social support from an animal or by strengthening the alliance between the patient and the treatment provider. To test these assumptions, we examined the effects of the presence of a dog on experimentally induced pain in a pain assessment and a pain therapy context. Hundred thirty-two healthy participants were randomly assigned to the conditions "pain," "pain + dog," "pain + placebo," or "pain + placebo + dog." We collected baseline and posttreatment measurements of heat-pain tolerance and the heat-pain threshold and of the corresponding subjective ratings of heat-pain intensity and unpleasantness as well as of participants' perceptions of the study investigator. The primary outcome was heat-pain tolerance. The presence of the dog did not influence the primary outcome ("pain" vs. "pain + dog": difference = 0.04, CI = -0.66 to 0.74, p = 0.905; "pain + placebo" vs. "pain + placebo + dog": difference = 0.43, CI = -0.02 to 0.88, p = 0.059). Participants did also not perceive the study investigator to be more trustworthy in the presence of the dog ("pain" vs. "pain + dog": difference = 0.10, CI = -0.67 to 0.87, p = 0.796; "pain + placebo" vs. "pain + placebo + dog": difference = 0.11, CI = -0.43 to 0.64, p = 0.695). The results indicate that the mere presence of a dog does not contribute to pain reduction and that the analgesic effects of AAI that previous studies have found is not replicated in our study as AAI did not increase perceived social support and had no effect on the alliance between the participant and the treatment provider. We assume that the animal most likely needs to be an integrated and plausible part of the treatment rationale so that participants are able to form a treatment-response expectation toward AAI

Animal-assisted psychotherapy for pediatric chronic pain: Case series of an open pilot study to test initial feasibility and potential efficacy

Chronic pain is a common complaint in children and adolescents, placing an enormous burden on individuals, their families, and the healthcare system. New innovative approaches for the treatment of pediatric chronic pain (PCP) are clearly warranted, as drop-out rates in intervention studies are high and it can be difficult to engage patients with PCP in therapy. Here, animal-assisted interventions (AAIs) might be promising, since there is preliminary evidence for the approach in adults with chronic pain, and AAIs are generally known to foster the therapeutic motivation of patients. To date, however, AAIs have not been examined in pediatric chronic pain

"There is a cat on our ward" : inpatient and staff member attitudes toward and experiences with cats in a psychiatric ward

The aim of this study was to investigate inpatient and staff member attitudes toward and experiences with ward cats, and identify possible mechanisms for how cats affect patient satisfaction in a psychiatric clinic. Thirty-three inpatients diagnosed with depression or psychosis residing on wards with and without cats and 17 staff members working on wards with cats participated in semi-structured interviews using a cross-sectional study design. Data analysis included descriptive statistics and correlations. The results showed that 17 out of 19 inpatients and all the staff members liked having a cat on their ward. Further, 12 out of 14 inpatients on wards without cats would like having a cat on their ward. Inpatient perceptions of the cat's impact on the ward atmosphere correlated significantly with their emotional relationship with the cat (; p; = 0.015,; r; = 0.561), how often they saw the cat (; p; = 0.002,; r; = 0.676), and if they liked cats in general (; p; = 0.041,; r; = 0.486). Our results highlight the positive attitudes of inpatients and staff members toward ward cats and the potential of ward cats to enhance patient satisfaction. This influence might be mediated by factors such as the frequency of contact, the relationship between each patient and the cat, and each patient's attitude toward cats in general

A comparative approach of simple green sample preparation methods based on optimization strategies for nutrient analysis of food

The chemical composition is regarded as the most important indicator to evaluate food quality and its nutritional value. Elemental composition (major, minor, trace and rare earth elements) reflects the particular environment and can provide uniquely representative fingerprints, making significant progress in authenticity studies. However, when using spectrochemical instrumental methods for inorganic trace analysis in solid samples, it is necessary to obtain a representative solution with the analytes. Sample preparation is a challenging step in the analytical procedure, where wet digestion sample preparation methods are widely used in analytical chemistry. In this context, Green Analytical Chemistry (GAC) searches for cheaper, more efficient and accurate greener alternatives, developing simple and inexpensive methods for analytes qualitative and/or quantitative determination. There is still a constant search for new alternatives for time-efficient pretreatments, reducing operating steps, contamination and reagent concentrations. In order to comply with GAC recommendations, the aim of this work was to develop and optimize analytical strategies for complex samples preparation extracting with dilute reagents and assisted by ultrasound (USAE) and infrared (IRAE) radiations for multielemental determination by Microwave induced plasma atomic emission spectrometry (MIP OES). Likewise, a comparative analysis was carried out between the two procedures under their optimal conditions and on three different samples (animal feed, grapes and pork meat). The experimental optimization of each sample preparation system was carried out through a face centered central composite design (FC-CCD), assessing 4 and 5 factors for IRAE and USAE, respectively. Dissolved organic carbon (DOC), residual acidity (RA) and solid residue (SR) were evaluated as responses employing desirability function. Thus, response surface methodology was implemented to find the best combination of mass, diluted reagents (HNO3 and H2O2), time and temperature in order to minimize the studied responses for elemental extraction in analyzed samples. The selected factors were evaluated in a range of 100-500 mg (sample mass), 15-45 min (heating time), 2-7 M (HNO3 concentration) and 10-30 % (H2O2 concentration) for both designs and the temperature between 30-60°C was considered in the USAE design while for the IRAE it was kept constant at 190°C. The comparison between both methodologies revealed a better performance of IRAE than USAE for the parameters studied, achieving lower values for DOC, RA and SR, which are statistically significant for the1772three samples under study. On the other hand, different optimal conditions were acquired for the different samples, evidencing that the sample preparation also depends on the sample under study. For this reason, after their experimental validation, the optimal combinations of IRAE design for each sample were selected to elucidate sample composition through multielemental analysis. Acquired results were in accordance with guidelines values.Fil: Jofre, Cora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Giacomino, Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Wagner, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Zaldarriaga Heredia, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Montemerlo, Antonella Evelin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Camiña, José Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Azcarate, Silvana Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Savio, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaVIII Congreso Internacional de Ciencia y Tecnología de los AlimentosCordobaArgentinaMinisterio de Ciencia y Tecnologí

Effects of animal-assisted therapy on social behaviour in patients with acquired brain injury : a randomised controlled trial

Animal-assisted therapy (AAT) is increasingly used to address impaired social competence in patients with acquired brain injury. However, the efficacy of AAT has not been tested in these patients. We used a randomised, controlled within subject trial to determine the effects of AAT on social competence in patients undergoing stationary neurorehabilitation. Participants received both AAT sessions and paralleled conventional therapy sessions. The patients' social behaviour was systematically coded on the basis of video recordings of therapy sessions. Moreover, mood, treatment motivation and satisfaction was measured during each therapy session. We analysed 222 AAT and 219 control sessions of 19 patients with linear mixed models. Patients showed a significantly higher amount of social behaviour during AAT. Furthermore, patients' positive emotions, verbal and non-verbal communication, mood, treatment motivation and satisfaction were increased in the presence of an animal. Neutral emotions were reduced but no effect was found regarding negative emotions. Our results show that AAT increases aspects of social competence and leads to higher emotional involvement of patients with acquired brain injury, reflected in higher social engagement, motivation and satisfaction during a therapeutic session

EGFL7 loss correlates with increased VEGF-D expression, upregulating hippocampal adult neurogenesis and improving spatial learning and memory

Correction: Volume: 80 Issue: 8 DOI: 10.1007/s00018-023-04835-3 Article Number: 201 Published: AUG 2023Neural stem cells reside in the subgranular zone, a specialized neurogenic niche of the hippocampus. Throughout adulthood, these cells give rise to neurons in the dentate gyrus, playing an important role in learning and memory. Given that these core cognitive processes are disrupted in numerous disease states, understanding the underlying mechanisms of neural stem cell proliferation in the subgranular zone is of direct practical interest. Here, we report that mature neurons, neural stem cells and neural precursor cells each secrete the neurovascular protein epidermal growth factor-like protein 7 (EGFL7) to shape this hippocampal niche. We further demonstrate that EGFL7 knock-out in a Nestin-CreERT2-based mouse model produces a pronounced upregulation of neurogenesis within the subgranular zone. RNA sequencing identified that the increased expression of the cytokine VEGF-D correlates significantly with the ablation of EGFL7. We substantiate this finding with intraventricular infusion of VEGF-D upregulating neurogenesis in vivo and further show that VEGF-D knock-out produces a downregulation of neurogenesis. Finally, behavioral studies in EGFL7 knock-out mice demonstrate greater maintenance of spatial memory and improved memory consolidation in the hippocampus by modulation of pattern separation. Taken together, our findings demonstrate that both EGFL7 and VEGF-D affect neurogenesis in the adult hippocampus, with the ablation of EGFL7 upregulating neurogenesis, increasing spatial learning and memory, and correlating with increased VEGF-D expression.Peer reviewe

Silane-Mediated Expansion of Domains in Si-Doped κ-Ga2O3 Epitaxy and its Impact on the In-Plane Electronic Conduction

Unintentionally doped (001)-oriented orthorhombic κ-Ga2O3 epitaxial films on c-plane sapphire substrates are characterized by the presence of ≈ 10 nm wide columnar rotational domains that can severely inhibit in-plane electronic conduction. Comparing the in- and out-of-plane resistance on well-defined sample geometries, it is experimentally proved that the in-plane resistivity is at least ten times higher than the out-of-plane one. The introduction of silane during metal-organic vapor phase epitaxial growth not only allows for n-type Si extrinsic doping, but also results in the increase of more than one order of magnitude in the domain size (up to ≈ 300 nm) and mobility (highest µ ≈ 10 cm2V−1s−1, with corresponding lowest ρ ≈ 0.2 Ωcm). To qualitatively compare the mean domain dimension in κ-Ga2O3 epitaxial films, non-destructive experimental procedures are provided based on X-ray diffraction and Raman spectroscopy. The results of this study pave the way to significantly improved in-plane conduction in κ-Ga2O3 and its possible breakthrough in new generation electronics. The set of cross-linked experimental techniques and corresponding interpretation here proposed can apply to a wide range of material systems that suffer/benefit from domain-related functional properties

'We don't know for sure':discussion of uncertainty concerning multigene panel testing during initial cancer genetic consultations

Pre-test counseling about multigene panel testing involves many uncertainties. Ideally, counselees are informed about uncertainties in a way that enables them to make an informed decision about panel testing. It is presently unknown whether and how uncertainty is discussed during initial cancer genetic counseling. We therefore investigated whether and how counselors discuss and address uncertainty, and the extent of shared decision-making (SDM), and explored associations between counselors' communication and their characteristics in consultations on panel testing for cancer. For this purpose, consultations of counselors discussing a multigene panel with a simulated patient were videotaped. Simulated patients represented a counselee who had had multiple cancer types, according to a script. Before and afterwards, counselors completed a survey. Counselors' uncertainty expressions, initiating and the framing of expressions, and their verbal responses to scripted uncertainties of the simulated patient were coded by two researchers independently. Coding was done according to a pre-developed coding scheme using The Observer XT software for observational analysis. Additionally, the degree of SDM was assessed by two observers. Correlation and regression analyses were performed to assess associations of communicated uncertainties, responses and the extent of SDM, with counselors' background characteristics. In total, twenty-nine counselors, including clinical geneticists, genetic counselors, physician assistants-in-training, residents and interns, participated of whom working experience varied between 0 and 25 years. Counselors expressed uncertainties mainly regarding scientific topics (94%) and on their own initiative (95%). Most expressions were framed directly (77%), e.g. We don't know, and were emotionally neutral (59%; without a positive/negative value). Counselors mainly responded to uncertainties of the simulated patient by explicitly referring to the uncertainty (69%), without providing space for further disclosure (66%). More experienced counselors provided less space to further disclose uncertainty (p <0.02), and clinical geneticists scored lower on SDM compared with other types of counselors (p <0.03). Our findings that counselors mainly communicate scientific uncertainties and use space-reducing responses imply that the way counselors address counselees' personal uncertainties and concerns during initial cancer genetic counseling is suboptimal

Lack of genotype-phenotype correlation in basal cell nevus syndrome:A Dutch multicenter retrospective cohort study

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