12 research outputs found
Recommended from our members
The "Adequacy" of world recreation resources
p. 21-28 from Forests and future resource conflicts
Recommended from our members
Forests and future resource conflicts
In February of 1975, the School of Forestry sponsored a series of lectures and discussions on the topic, "Forests of the World-Future Resources Conflicts." The objectives of these presentations were to examine prospects for future forest use, the demands that will be made on forest resources of all sorts, the possibilities we have for meeting these demands, and what we must do to meet the ensuing problems.
We present here several of the papers having the most general and unusual interest
An analysis of the growing stock and structure of a tract of uneven-aged hardwoods in southern Michi
Master of ScienceForestryUniversity of Michiganhttp://deepblue.lib.umich.edu/bitstream/2027.42/115813/1/39015003272724.pd
Predicting the durability of forest recreation sites in northern Utah : preliminary results /
no.11
θ Defensins Protect Cells from Infection by Herpes Simplex Virus by Inhibiting Viral Adhesion and Entry
We tested the ability of 20 synthetic θ defensins to protect cells from infection by type 1 and type 2 herpes simplex viruses (HSV-1 and -2, respectively). The peptides included rhesus θ defensins (RTDs) 1 to 3, originally isolated from rhesus macaque leukocytes, and three peptides (retrocyclins 1 to 3) whose sequences were inferred from human θ-defensin (DEFT) pseudogenes. We also tested 14 retrocyclin analogues, including the retro, enantio, and retroenantio forms of retrocyclin 1. Retrocyclins 1 and 2 and RTD 3 protected cervical epithelial cells from infection by both HSV serotypes, but only retrocyclin 2 did so without causing cytotoxicity or requiring preincubation with the virus. Surface plasmon resonance studies revealed that retrocyclin 2 bound to immobilized HSV-2 glycoprotein B (gB2) with high affinity (K(d), 13.3 nM) and that it did not bind to enzymatically deglycosylated gB2. Temperature shift experiments indicated that retrocyclin 2 and human α defensins human neutrophil peptide 1 (HNP 1) to HNP 3 protected human cells from HSV-2 by different mechanisms. Retrocyclin 2 blocked viral attachment, and its addition during the binding or penetration phases of HSV-2 infection markedly diminished nuclear translocation of VP16 and expression of ICP4. In contrast, HNPs 1 to 3 had little effect on binding but reduced both VP16 transport and ICP4 expression if added during the postbinding (penetration) period. We recently reported that θ defensins are miniature lectins that bind gp120 of human immunodeficiency virus type 1 (HIV-1) with high affinity and inhibit the entry of R5 and X4 isolates of HIV-1. Given its small size (18 residues), minimal cytotoxicity, lack of activity against vaginal lactobacilli, and effectiveness against both HSV-2 and HIV-1, retrocyclin 2 provides an intriguing prototype for future topical microbicide development