Frequency and distribution of ABO and Rh blood groups among blood donors in tertiary care hospital of South Gujarat, India

Background: Since it was discovered by Karl Landsteiner, the ABO blood group system is the most important blood group system in Transfusion Medicine. The blood group systems are also very important in population genetic studies, researching population migration patterns as well as resolving certain medico-legal issues, particularly disputed parentage. This study was carried out with an objective to provide data regarding gene frequency and distribution of ABO and Rh blood groups among blood donors in South Gujarat, India.Methods: Data of 40732 blood donors were retrospectively collected and analyzed regarding ABO and Rh blood groups from May-2011 to April-2016 and reported in simple numbers and percentage. Blood group of the blood donors was determined by forward and reverse methods with the help of commercially available standard monoclonal antisera by test tube and column agglutination techniques in required cases.Results: The most common blood group among donors was B (34.43%) followed by O (32.26%), A (24.35%), while the least prevalent blood group was AB (8.94%). Rh positivity among donors was (95.12%). Rests were Rh Negative (4.87%). The estimated gene frequencies were 0.1844 for IA (p), 0.2477 for IB (q) and 0.5679 for IO (r).Conclusions: The most common blood group in donors was B positive and least common was AB negative

Prevalence and trends of transfusion transmitted infections among blood donors of blood bank attached to government hospital of South Gujarat, India

Background: Though the blood transfusion is lifesaving, it is never risk free and carries potential risk for transfusion transmitted infections (TTIs).The objective of present study is to assess the prevalence and trend of TTIs among blood donors in the blood bank attached to tertiary level government hospital and its comparison with national data and other studies of different regions.Methods: Retrospective cross-sectional study was done including healthy blood donor of 18-65 years from May 2011 to April 2016. TTIs testing were done with 3rd generation ELISA for HIV, HBV and HCV and HIV testing was also done with 4th generation ELISA from the year 2014 onwards. Syphilis screening was done either by RPR, TPHA strip test or ELISA and Malaria parasite was tested either by peripheral smear or rapid test.Results: From 40971 donors in study, 550 (1.34%) donors were sero-reactive from which 400 (0.98%) donors were reactive for HBV, 67 (0.16%) were for syphilis, 40 (0.098%) were for HCV, 33 (0.081%) were reactive for HIV and 10 (0.024%) were malaria positive. During the study period four donors showed co infection for HIV and HBV. Most common age group to be sero reactive in the present study is 21-30 years of age (43.09% of total).Conclusions: Prevalence of TTIs in the present study was lower as compare to other studies of the India. The reasons behind this finding may be better pre-donation counseling, better life style, higher proportion of voluntary blood donation (> 99%) and high pre-donation deferral rate (11.17%)

Transfusion effect of random donor platelet and single donor platelet in thrombocytopenic patients at tertiary care hospital of South Gujarat

Background: Platelet transfusion plays a key role in therapy for the patients with thrombocytopenia. Superiority of Single donor platelet (SDP) over Random donor platelet (RDP) transfusions is largely assumed, but unproven. Platelet Rich Plasma-Platelet concentrate (PRP-PC) and Apheresis-PC were prepared and their therapeutic efficacy were assessed in thrombocytopenic patients.Methods: This study included 60 transfusion episodes consisting of 30 SDP and 30 RDP (147units of RDP). The post transfusion efficacy of transfused platelets was assessed at 1 hour and 24 hours by corrected count increment (CCI) and percentage recovery (PR). Paired ‘t’-test was used for statistical analysis and a probability of p<0.05 was used to reject null hypothesis.Results: The mean platelet dose of SDP (n=30) and RDP (n=30) was 2.86±1.05 x 1011 and 2.36±0.54 x 1011 respectively. The mean platelet increments of SDP at 1 hour and 24 hours were 38±18.1 x 103/μl and 37.3±20.7x 103/μl. The mean platelet increments of RDP at 1 hour and 24 hours were 28.5±11.4 x 103/μl and 26 ±11.6 x 103/μl. The mean CCI of SDP at 1hour and 24 hours were 21.4 ±7.3 x 103/μl and 20.8±7.4 x 103/μl respectively. The mean CCI of RDP at 1hour and 24 hours were 18.5±6.3x 103/μl and 17.4±7.6 x 103/μl respectively.Conclusions: Post-transfusion increments were significantly higher in patients who received SDP as compared to RDP, but the CCI and PR were comparable in both groups of patients

Frequency and distribution of ABO and Rh blood groups among blood donors in tertiary care hospital of South Gujarat, India

Background: Since it was discovered by Karl Landsteiner, the ABO blood group system is the most important blood group system in Transfusion Medicine. The blood group systems are also very important in population genetic studies, researching population migration patterns as well as resolving certain medico-legal issues, particularly disputed parentage. This study was carried out with an objective to provide data regarding gene frequency and distribution of ABO and Rh blood groups among blood donors in South Gujarat, India.Methods: Data of 40732 blood donors were retrospectively collected and analyzed regarding ABO and Rh blood groups from May-2011 to April-2016 and reported in simple numbers and percentage. Blood group of the blood donors was determined by forward and reverse methods with the help of commercially available standard monoclonal antisera by test tube and column agglutination techniques in required cases.Results: The most common blood group among donors was B (34.43%) followed by O (32.26%), A (24.35%), while the least prevalent blood group was AB (8.94%). Rh positivity among donors was (95.12%). Rests were Rh Negative (4.87%). The estimated gene frequencies were 0.1844 for IA (p), 0.2477 for IB (q) and 0.5679 for IO (r).Conclusions: The most common blood group in donors was B positive and least common was AB negative

Prevalence and trends of transfusion transmitted infections among blood donors of blood bank attached to government hospital of South Gujarat, India

Background: Though the blood transfusion is lifesaving, it is never risk free and carries potential risk for transfusion transmitted infections (TTIs).The objective of present study is to assess the prevalence and trend of TTIs among blood donors in the blood bank attached to tertiary level government hospital and its comparison with national data and other studies of different regions.Methods: Retrospective cross-sectional study was done including healthy blood donor of 18-65 years from May 2011 to April 2016. TTIs testing were done with 3rd generation ELISA for HIV, HBV and HCV and HIV testing was also done with 4th generation ELISA from the year 2014 onwards. Syphilis screening was done either by RPR, TPHA strip test or ELISA and Malaria parasite was tested either by peripheral smear or rapid test.Results: From 40971 donors in study, 550 (1.34%) donors were sero-reactive from which 400 (0.98%) donors were reactive for HBV, 67 (0.16%) were for syphilis, 40 (0.098%) were for HCV, 33 (0.081%) were reactive for HIV and 10 (0.024%) were malaria positive. During the study period four donors showed co infection for HIV and HBV. Most common age group to be sero reactive in the present study is 21-30 years of age (43.09% of total).Conclusions: Prevalence of TTIs in the present study was lower as compare to other studies of the India. The reasons behind this finding may be better pre-donation counseling, better life style, higher proportion of voluntary blood donation (&gt; 99%) and high pre-donation deferral rate (11.17%)