Formation of virus-like particles from human cell lines exclusively expressing Influenza Neuraminidase

The minimal virus requirements for the generation of influenza virus-like particle (VLP) assembly and budding were reassessed. Using neuraminidase (NA) from the H5N1 and H1N1 subtypes, it was found that the expression of NA alone was sufficient to generate and release VLPs. Biochemical and functional characterization of the NA-containing VLPs demonstrated that they were morphologically similar to influenza virions. The NA oligomerization was comparable to that of the live virus, and the enzymic activity, whilst not required for the release of NA-VLPs, was preserved. Together, these findings indicate that NA plays a key role in virus budding and morphogenesis, and demonstrate that NA-VLPs represent a useful tool in influenza research. © 2010 SGM.link_to_OA_fulltex

Underground utilities imaging and diagnosis

202109 bcvcVersion of RecordPublishe

Characterization of complex dielectric permittivity of concrete by gpr numerical simulation and spectral analysis

202209 bcvcAccepted ManuscriptRGCPublishe

Field validation of water-pipe leakage detection through spatial and time-lapse analysis of GPR wave velocity

202207 bcfcAccepted ManuscriptRGCPublishe

Subsurface diagnosis with time-lapse GPR slices and change detection algorithms

202101 bcrcVersion of RecordPublishe

A lab study of coupling effects of electromagnetic induction on underground utilities

202305 bckwAccepted ManuscriptOthersThe Hong Kong Polytechnic UniversityPublishe

GPR pattern recognition of shallow subsurface air voids

202207 bcfcAccepted ManuscriptRGCPublishe

Detection and imaging of city’s underground void by GPR

2017 9th International Workshop on Advanced Ground Penetrating Radar [IWAGPR], 28-30 June 2017, Edinburgh, UK.202305 bckwAccepted ManuscriptOthersHong Kong Polytechnic UniversityPublishe

Time-frequency spectral analysis of step frequency continuous wave and impulse ground penetrating radar

2016 16th International Conference on Ground Penetrating Radar (GPR), 13-16 June 2016, Hong Kong, China202305 bckwAccepted ManuscriptRGCPublishe