112 research outputs found

    Assessing the health and hygiene performance of apartment buildings

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    The recent global outbreak of Severe Acute Respiratory Syndrome has aroused public concern on environmental health and hygiene. Develops a practical assessment scheme for assessing the health and hygiene performance of apartment buildings in Hong Kong. The scheme involves assessing a hierarchy of building factors that have a bearing on environmental qualities, and thus occupants' health. Proposes an index method to integrate the assessment outcomes into a simple and user- friendly performance indicator for public consumption. The index can inform the public of the health and hygiene risk of different buildings and facilitate building owners, developers, and government bodies to make more informed and socially responsible decisions on environmental health and hygiene improvement. Although the assessment scheme is tailored for the institutional and cultural settings of Hong Kong, the assessment framework for the development of the scheme is also applicable to other cities.published_or_final_versio

    What Is in a Name?

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    Characterization and Gene Expression Profiling of Five New Human Embryonic Stem Cell Lines Derived in Taiwan

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    Many human embryonic stem cell (hESC) lines have been reported, but only a few of them have been fully characterized. In this report, five new hESC lines were derived from 32 discarded blastocysts in Taiwan, and these lines were continuously cultured on mitotically inactivated mouse embryonic fibroblast (MEF) feeder layer in the hESC medium for more than 44 passages and underwent freezing/thawing processes. All five hESC lines expressed characteristic undifferentiated hESC markers, such as SSEA-4, TRA-1-81, alkaline phosphatase, TERT, and the transcription factors POU5F1 (OCT4) and NANOG. hESC lines T1 and T3 possess normal female karyotypes, whereas lines T4 and T5 are normal male, but line T2 is male trisomy 12 (47XY,+12). hESC lines T1, T2, T3, and T5 were able to produce teratomas in severe combined immunodeficient (SCID) mice, and line T4 could only form embryoid bodies (EBs) in vitro. Global gene expression profiles of these five newly derived hESC lines were analyzed using the Affymetrix human genome U133 plus 2.0 GeneChip. The results showed that 4,145 transcripts, including 19% of unknown functions, were detected in all five hESC lines. Comparison of the 4,145 genes commonly expressed in the five hESC lines with those genes expressed in teratomas produced by the hESC line T1 and placenta revealed 40 genes exclusively expressed in all five hESC lines. These 40 genes include the previously reported stemness genes, such as POU5F1 (OCT4), NANOG, TDGF1 (CRIPTO), SALL4, LECT1, and BUB1 responsible for self-renewal and pluripotent differentiation. The global gene expression analysis also indicated that the transforming growth factor-β (TGF-β)/activin branch components inhibin BC, ACVR2A, ACVR1 (ALK2), TGFBR1 (ALK5), and SMAD2 were found to be highly expressed in undifferentiated states of these five hESC lines and decreased upon differentiation. In short, the hESC nature of these five hESC lines is supported by the undifferentiated state, extensive renewal capacity, and pluripotency, including the ability to form teratomas and/or EBs. These cell lines will be useful for human embryonic stem cell biology and drug development

    EBNA1-targeted probe for the imaging and growth inhibition of tumours associated with the Epstein–Barr virus

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    202209 bckwAccepted ManuscriptRGCOthersHong Kong Baptist University; EPSRC (Durham University, DTA award);Publishe
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