Lymnaea schirazensis, an Overlooked Snail Distorting Fascioliasis Data: Genotype, Phenotype, Ecology, Worldwide Spread, Susceptibility, Applicability

BACKGROUND: Lymnaeid snails transmit medical and veterinary important trematodiases, mainly fascioliasis. Vector specificity of fasciolid parasites defines disease distribution and characteristics. Different lymnaeid species appear linked to different transmission and epidemiological patterns. Pronounced susceptibility differences to absolute resistance have been described among lymnaeid populations. When assessing disease characteristics in different endemic areas, unexpected results were obtained in studies on lymnaeid susceptibility to Fasciola. We undertook studies to understand this disease transmission heterogeneity. METHODOLOGY/PRINCIPAL FINDINGS: A ten-year study in Iran, Egypt, Spain, the Dominican Republic, Mexico, Venezuela, Ecuador and Peru, demonstrated that such heterogeneity is not due to susceptibility differences, but to a hitherto overlooked cryptic species, Lymnaea schirazensis, confused with the main vector Galba truncatula and/or other Galba/Fossaria vectors. Nuclear rDNA and mtDNA sequences and phylogenetic reconstruction highlighted an old evolutionary divergence from other Galba/Fossaria species, and a low intraspecific variability suggesting a recent spread from one geographical source. Morphometry, anatomy and egg cluster analyses allowed for phenotypic differentiation. Selfing, egg laying, and habitat characteristics indicated a migration capacity by passive transport. Studies showed that it is not a vector species (n = 8572 field collected, 20 populations): snail finding and penetration by F. hepatica miracidium occur but never lead to cercarial production (n = 338 experimentally infected). CONCLUSIONS/SIGNIFICANCE: This species has been distorting fasciolid specificity/susceptibility and fascioliasis geographical distribution data. Hence, a large body of literature on G. truncatula should be revised. Its existence has henceforth to be considered in research. Genetic data on livestock, archeology and history along the 10,000-year post-domestication period explain its wide spread from the Neolithic Fertile Crescent. It is an efficient biomarker for the follow-up of livestock movements, a crucial aspect in fascioliasis emergence. It offers an outstanding laboratory model for genetic studies on susceptibility/resistance in F. hepatica/lymnaeid interaction, a field of applied research with disease control perspectives

A new magnetostratigraphic framework for the Lower Miocene (Burdigalian/Ottnangian, Karpatian) in the North Alpine Foreland Basin

Oligocene–Miocene chronostratigraphic correlations within the Paratethys domain are still highly controversial. This study focuses on the late Early Miocene of the Swiss and S-German Molasse Basin (Late Burdigalian, Ottnangian–Karpatian). Previous studies have published different chronologies for this time interval that is represented by the biostratigraphically well constrained Upper Marine Molasse (OMM, lower and middle Ottnangian), Upper Brackish Molasse (OBM, Grimmelfingen and Kirchberg Formations, middle and upper Ottnangian to lower Karpatian, MN 4a–MN 4b) and Upper Freshwater Molasse (OSM, Karpatian–Badenian, MN 5). Here, we suggest a new chronostratigraphic framework, based on integrated magneto-litho-biostratigraphic studies on four sections and three boreholes. Our data indicate that the OBM comprises chrons 5D.1r and 5Dn (Grimmelfingen Fm), chron 5Cr (lower Kirchberg Fm) and the oldest part of chron 5Cn.3n (upper Kirchberg Fm). The OSM begins during chron 5Cn.3n, continues through 5Cn, and includes a long reversed segment that can be correlated to chron 5Br. The OMM-OSM transition was completed at 16.0 Ma in the Swiss Molasse Basin, while the OBM-OSM changeover ended at 16.6 Ma in the S-German Molasse Basin. As the lower Kirchberg Fm represents a facies of the Ottnangian, our data suggest that the Ottnangian–Karpatian boundary in the Molasse Basin is approximately at 16.8 Ma, close to the 5Cr–5Cn.3n magnetic reversal, and thus 0.4 Myr younger than the inferred age of 17.2 Ma used in recent Paratethys time scales. Notably, this would not be problematic for the Paratethys stratigraphy, because chron 5Cr is mainly represented by a sedimentation gap in the Central Paratethys. We also realise, however, that additional data is still required to definitely solve the age debate concerning this intriguing time interval in the North Alpine Foreland Basin. We dedicate this work to our dear friend and colleague Jean-Pierre Berger (8 July 1956–18 January 2012)