11,903 research outputs found
Femtosecond spectroscopy of the first events of the photochemical cycle in bacteriorhodopsin
The first steps in the photochemistry of bacteriorhodopsin (BR) are investigated with light pulses of 160 fs duration. Four samples are studied: (i) the purple membrane, (ii) deuterated purple membrane, (iii) BR trimers and (iv) BR monomers. In all samples the first intermediate J is formed within 430±50 fs. No isotope effect is observed in the formation of J upon deuteration, in contrast to previous reports with much higher excitation energies. Thus proton movement to or from the retinal Schiff's base is not relevant during the first step. Comparing the data for trimeric and monomeric BR suggests an upper limit of 50 fs for the transfer of excitation energy from the excitonically coupled trimer to a single retinal chromophore
Early picosecond events in the photo cycle of Bacteriorhodopsin
The primary processes of the photochemical cycle of light-adapted bacteriorhodopsin (BR) were studied by various experimental techniques with a time resolution of 5 × 10-13 s. The following results were obtained. (a) After optical excitation the first excited singlet state S1 of bacteriorhodopsin is observed via its fluorescence and absorption properties. The population of the excited singlet state decays with a lifetime τ1 of ~0.7 ps (430 ± 50 fs) (52). (b) With the same time constant the first ground-state intermediate J builds up. Its absorption spectrum is red-shifted relative to the spectrum of BR by ~30 nm. (c) The second photoproduct K, which appears with a time constant of τ2 = 5 ps shows a red-shift of 20 nm, relative to the peak of BR. Its absorption remains constant for the observation time of 300 ps. (d) Upon suspending bacteriorhodopsin in D2O and deuterating the retinal Schiff base at its nitrogen (lysine 216), the same photoproducts J and K are observed. The relaxation time constants τ1 and τ2 remain unchanged upon deuteration within the experimental accuracy of 20%
The reaction of Pt (PPh3)2(C2H4) with tetrachlorocyclopropene. The molecular structure of Pt(PPh3)Cl2(η1-C(Cl)=C(Cl)-C(H)(Cl)PPh3)
Optical picosecond studies of bacteriorhodopsin containing a sterically fixed retinal
The photochemical behaviour of an analogous bacteriorhodopsin (9,12-Ph-BR) which contains the sterically fixed 9,12-phenylretinal has been investigated with picosecond spectroscopy. The following results have been obtained. No ground-state intermediate photoproduct is found in agreement with the previous observation that 9,12-Ph-BR does not exhibit proton pumping under illumination. The excited singlet state has a lifetime of τS = 10 ± 2 ps. This lifetime agrees favourably with the value calculated from the radiative lifetime τrad = 6.2 ns and the fluorescence quantum efficiency of 1.2·10−3. Excited-state absorption occurs which results in fluorescence in the ultraviolet region. These various observations differ drastically from the corresponding findings on bacteriorhodopsin. Most important for an understanding of the differences is the fact that 9,12-phenylretinal does not isomerize in the protein's binding site in contrast to retinal. Our data therefore suggest that the formation of the intermediate K observed in bacteriorhodopsin is accompanied by the all-trans to 13-cis isomerization
On the mechanism of action of the cytostatic drug anguidine and of the immunosuppressive agent ovalicin, two sesquiterpenes from fungi
Leukocyte margination in alveolar capillaries: Interrelationship with functional capillary geometry and microhemodynamics
The pulmonary capillary microvasculature harbors a large pool of intravascularly marginated leukocytes. In this study, we investigated the interrelationship of leukocyte margination with characteristics of functional capillary geometry and microhemodynamics in alveolar capillary networks. In 22 anesthetized rabbits we assessed functional capillary density, average capillary length, red blood cell velocity and leukocyte kinetics in alveolar capillary networks in vivo by intravital fluorescence microscopy. In alveolar wall areas of 12,800 +/- 1,800 mu m(2), we detected 3.6 +/- 0.5 sticking leukocytes and 21.0 +/- 1.9 functional capillary segments with an average capillary length of 35.7 +/- 2.1 mu m. We calculated that approximately 15% of functional capillary segments are blocked by marginated leukocytes. Leukocyte margination was predominantly observed in capillary networks characterized by a high functional capillary density, short capillary segments and low red blood cell velocities. The multitude of interconnected capillary channels in these networks may allow alveolar blood flow to bypass marginated leukocytes. Hence, this interrelationship may be relevant for maintenance of adequate alveolar perfusion and low capillary network resistance despite excessive leukocyte margination in the pulmonary microvasculature. Local microhemodynamic factors may play a regulatory role in the spatial distribution of leukocyte margination
Thermometry and Refrigeration in a Two-Component Mott Insulator of Ultracold Atoms
Interesting spin Hamiltonians can be realized with ultracold atoms in a
two-component Mott insulator (2CMI). It was recently demonstrated that the
application of a magnetic field gradient to the 2CMI enables new techniques of
thermometry and adiabatic cooling. Here we present a theoretical description
which provides quantitative analysis of these two new techniques. We show that
adiabatic reduction of the field gradient is capable of cooling below the Curie
or N\'eel temperature of certain spin ordered phases.Comment: 5 pages, 5 figures (v4): Added journal referenc
Spin gradient thermometry for ultracold atoms in optical lattices
We demonstrate spin gradient thermometry, a new general method of measuring
the temperature of ultracold atoms in optical lattices. We realize a mixture of
spins separated by a magnetic field gradient. Measurement of the width of the
transition layer between the two spin domains serves as a new method of
thermometry which is observed to work over a broad range of lattice depths and
temperatures, including in the Mott insulator regime. We demonstrate the
thermometry in a system of ultracold rubidium atoms, and suggest that
interesting spin physics can be realized in this system. The lowest measured
temperature is 1 nK, indicating that the system has reached the quantum regime,
where insulating shells are separated by superfluid layers.Comment: 5 pages, 3 figures, minor edits for clarit
Spin gradient demagnetization cooling of ultracold atoms
A major goal of ultracold atomic physics is quantum simulation of spin
Hamiltonians in optical lattices. Progress towards this goal requires the
attainment of extremely low temperatures. Here we demonstrate a new cooling
method which consists of applying a time-varying magnetic field gradient to a
spin mixture of ultracold atoms. We have used this method to prepare isolated
spin distributions at positive and negative spin temperatures of +/-50
picokelvin. The spin system can also be used to cool other degrees of freedom,
and we have used this coupling to reduce the temperature of an apparently
equilibrated sample of rubidium atoms in a Mott insulating state to 350
picokelvin. These are the lowest temperatures ever measured in any system.Comment: 4 pages, 4 figures; (v4) Shortened, added journal re
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