22 research outputs found
A Recombinant Influenza A Virus Expressing Domain III of West Nile Virus Induces Protective Immune Responses against Influenza and West Nile Virus
West Nile virus (WNV) continues to circulate in the USA and forms a threat to the rest of the Western hemisphere. Since methods for the treatment of WNV infections are not available, there is a need for the development of safe and effective vaccines. Here, we describe the construction of a recombinant influenza virus expressing domain III of the WNV glycoprotein E (Flu-NA-DIII) and its evaluation as a WNV vaccine candidate in a mouse model. FLU-NA-DIII-vaccinated mice were protected from severe body weight loss and mortality caused by WNV infection, whereas control mice succumbed to the infection. In addition, it was shown that one subcutaneous immunization with 105 TCID50 Flu-NA-DIII provided 100% protection against challenge. Adoptive transfer experiments demonstrated that protection was mediated by antibodies and CD4+T cells. Furthermore, mice vaccinated with FLU-NA-DIII developed protective influenza virus-specific antibody titers. It was concluded that this vector system might be an attractive platform for the development of bivalent WNV-influenza vaccines
West Nile virus genome with glycosylated envelope protein and deletion of alpha helixes 1, 2 and 4 in the capsid gene is non-infectious and efficiently secretes sub-viral particles
Flavivirus genomes with deletions in the capsid (C) gene are attractive vaccine candidates, as they secrete highly immunogenic subviral particles (SVPs) without generating infectious virus. Here, we report that cytomegalovirus promoter-driven cDNA of West Nile virus Kunjin (KUNV) containing a glycosylation motif in the envelope (E) gene and a combined deletion of alpha helices 1, 2, and 4 in C produces significantly more SVPs than KUNV cDNAs with nonglycosylated E and various other deletions in C
Os benefĂcios dos exercĂcios resistidos na melhoria da capacidade funcional e saĂșde dos paraplĂ©gicos
Os exercĂcios resistidos (ER) tĂȘm-se mostrado como um dos recursos efetivos para a promoção da saĂșde e melhoria da capacidade funcional de paraplĂ©gicos. Entretanto, o nĂșmero limitado de estudos sobre a influĂȘncia dos ER para tal população, acaba por limitar as possibilidades de utilização desta prĂĄtica. O principal objetivo deste estudo foi realizar uma revisĂŁo sobre os benefĂcios dos ER na melhoria da capacidade funcional e saĂșde dos paraplĂ©gicos. Para tanto, foi realizada uma pesquisa na base de dados do Lilacs, Medline, SportDiscus, SciELO e Scholar Google com o intuito de selecionar as evidĂȘncias cientĂficas. Os estudos revisados demonstraram que programas de ER sĂŁo capazes de promover benefĂcios psicolĂłgicos, sociais e fĂsicos, dentre eles a melhoria da aderĂȘncia ao exercĂcio, melhoria dos nĂveis de força, resistĂȘncia e potĂȘncia, aumento do metabolismo e da melhoria do perfil lipĂdico aterogĂȘnico, e a redução de dores nos ombros em paraplĂ©gicos. Os ER sĂŁo benĂ©ficos e atĂ© mesmo necessĂĄrios para manter a capacidade funcional e saĂșde nesta população