Molecular cloning of Na+-ATPase cDNA from a marine alga, Heterosigma akashiwo

AbstractWe cloned novel Na+-ATPase (HANA) cDNA from marine alga Heterosigma akashiwo. The full-length HANA cDNA was 4467 bp long and coded for a 1330 amino acid protein with a molecular weight of 146 306. The deduced product exhibited around 40% identity in amino acids with Na+/K+-ATPase α-subunits. A hydrophilic sequence of 285 amino acid residues that showed no homology with any sequence listed in databases existed in the M7–M8 junction of HANA. This is the first report on the primary structure of putative Na+-transporting ATPase from plant cells

KINEMATIC ANALYSIS ON THE PUNT KICK IN FOOTBALL GOALKEEPER

The present study aimed to investigate punt kicks by football goalkeepers on the basis of differences in the effort of the kick. Twelve experienced goalkeepers participated in the study. The participants were made aware that for the maximum distance trial (the 100% trial), they should send the ball as far as possible, and for the 80% and 60% trials they should have a more controlled approach. Punt kicks were divided into three phases: the phase of release of the ball from the left hand (BR), the phase of pivot foot ground-contact (LFC), and the phase of the ball impact (IMP). Right hip and knee joint angles were calculated. In comparison to lower intensity punt kicks, the higher effort punt kicks involved increasing the hip joint extension angle for the right foot during the backswing and lowering the knee joint angle of the right leg at the start of the forward swing, thereby producing forward swing velocity for the right foot

INFLUENCE OF THE BALL SPEED ON THE DISPLACEMENT OF THE CENTER OF GRAVITY DURING BASEBALL BATTING MOTION

The purpose of this study was to investigate the modification in batting motion with different pitching speeds focusing on differences in batting technical level. The subjects were 10 experienced university baseball player. The subjects batted toward the center field, both fastballs and slowballs, aimed near the center of the strike zone from a pitching machine. Data were collected using a three dimensional automatic motion analysis system (Vicon MX). The displacement of the center of gravity(CG) were computed. Significant differences were seen due to difference in pitching speed in unskilled player. Conversely, in skilled player, no significant difference was found in the movement of CG due to the difference in pitching speed. It was revealed that it was not preferable for movement of the CG to fluctuate by difference in pitching speeds

大腿四頭筋の等尺性収縮は、膝蓋上外側アプローチによる膝関節内注射の正確性を向上する

Background: Intra-articular injection is an important technique for treating rheumatoid arthritis and osteoarthritis of the knee. However, medication is often inaccurately injected outside of the joint. We devised an intra-articular injection method in which the needle is inserted into the suprapatellar bursa while the patient maintains isometric contraction of the quadriceps. This isometric contraction method is based on the concept that isometric contraction of the quadriceps induces contraction of the articularis genus muscle, thus expanding the lumen of the suprapatellar bursa. Methods: Intra-articular injections were performed on 150 osteoarthritic knees without effusion. The knees were alternately assigned to the isometric quadriceps method group (75 knees) and non-activated quadriceps method group (75 knees). Prior to joint injection, the anterior-posterior dimension of each suprapatellar bursa was measured to ascertain its expansion. The isometric quadriceps method was performed with the quadriceps and the articularis genus muscle maintained in a contracted state. The non-activated quadriceps method was performed in a relaxed state. Ultrasound guidance was not used for either method. Subsequently, an ultrasonic probe was used only to confirm whether the intra-articular injections were successful. We compared the accuracy of injections performed between the 2 groups. Results: Suprapatellar expansion was significantly larger (p < 0.001) using the isometric quadriceps method (2.1 ± 1.4 mm [range, 0 to 5 mm]) than using the non-activated quadriceps method (0.8 ± 0.7 mm [range, 0 to 2 mm]). The percentage of accurate intra-articular injections was significantly higher (p = 0.0287) using the isometric quadriceps method (93%) compared with the non-activated quadriceps method (80%). Conclusions: In comparison with the non-activated quadriceps method, the isometric quadriceps method led to a larger expansion of the suprapatellar bursa, which should lead to more accurate intra-articular injections. The isometric quadriceps method is effective in reducing inaccurate injections into the synovium or surrounding fatty tissues. Clinical relevance: Putting force on the quadriceps muscle increases the success rate of intra-articular injection of the knee. The results of this study could provide a clinically relevant injection technique for future treatment.博士（医学）・乙第1448号・令和元年12月5日Copyright © 2018 The Authors. Published by The Journal of Bone and Joint Surgery, Incorporated. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND https://creativecommons.org/licenses/by-nc-nd/4.0/), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal

慢性期末梢前庭障害に対する前庭リハビリテーションの身体活動量と主観的なめまい感に対する効果：6カ月間のランダム化比較試験

Introduction: The present study aimed to determine whether supervised vestibular rehabilitation therapy (VRT) by physical therapists (PTs) affects subjective dizziness in patients with chronic vestibular disorders, and whether supervised VRT-induced changes in subjective dizziness are related to the changes in physical activity levels in daily life. Methods: Patients (n = 47) with chronic peripheral vestibular disorders were randomly divided into the VRT group (n = 25) and control group (n = 22). Patients in the VRT group received weekly supervised visits from PTs for a period of 6 months. Every other month, both groups were advised by neuro-otologists to increase the amount of activity in their daily life. All patients wore an accelerometer device, which recorded their physical activity for seven successive days before the end of the intervention. Patients also completed the dizziness and unsteadiness questionnaires before and after the intervention. Results: Subjective dizziness decreased significantly regardless of whether supervised VRT was administered; however, dizziness evoked by social activity and head and body movements improved more significantly in the VRT group than in the control group. In the VRT group, there was a significant negative correlation between the increase in sedentary behavior and improvement in subjective dizziness, and a significant positive correlation between the increase in light physical activity and improvement in subjective dizziness at the second month of intervention. The VRT group showed a significantly higher rate of increase in light physical activity than the control group, after 6 months of intervention. Conclusion: Supervised VRT could be highly effective in treating subjective dizziness in patients with chronic peripheral vestibular disorders. We believe frequent (weekly) and medium-term (6 months) PT-guided interventions may be highly effective in enhancing physical activity in daily life, and may subsequently improve subjective dizziness in these patients. Trial registration: This clinical study was registered with University hospital Medical Information Network (identification number: 000028832). https://www.umin.ac.jp/博士（医学）・甲第878号・令和5年3月15

KINEMATIC ANALYSIS OF BASEBALL BATTING MOTION WHEN BATTING PITCHES WITH VARYING VELOCITIES

The purpose of this study was to identify effect of moving the center of gravity of the body and rotating the torso when batting pitches with varying velocity. The subjects were 10 experienced university baseball player. The subjects batted toward the center field, both fastballs and slowballs, aimed near the center of the strike zone from a pitching machine. Data were collected using a three dimensional automatic motion analysis system (Vicon MX). The rotation angle of the torso and displacement of the center of gravity were computed. Due to differences in the shoulder rotation from the latter half of stepping leg touchdown through impact, we believe that the motion is adapted to pitch differences starting from the latter half of stepping leg touchdown. Comparing the shift in the center of gravity of the body during fastballs and slowballs, the forward motion and downward sinking of the center of gravity were significantly larger for slowballs

Kinematic analysis of punt kick in football goalkeepers based on the level of kick effort

In the present study, we aimed to investigate the differences in punt kicks by football goalkeepers based on the level of effort required. Twelve experienced goalkeepers participated in the study. The participants were instructed to kick the ball as far as possible in the maximum distance trial (100% trial) and to have a more controlled approach for the 80% and 60% trials. Each punt kick was divided into three events: release of the ball from the left hand (BR), pivot foot ground-contact (LFC), and ball impact (IMP). Right lower limb joint velocity, right hip and knee joint angles, flight distance, ball velocity, and kick angle were calculated. The 80% and 100% trials yielded almost the same velocity for each part of the right leg; however, in the 60% trial, the level of kicking effort was managed by adjusting the velocity of the right ankle joint, starting from BR, in addition to adjustment of the velocity of the right knee joint at LFC. Compared to punt kicks with a lower level of effort, the punt kicks with a higher level of effort involved an increase in the hip joint extension angle for the right leg during the backswing and the lowering of the knee joint angle of the right leg at the start of the forward swing, thus producing forward swing velocity for the right foot