Genetic Parameters for Lean Growth Rate and Its Components in U.S. Landrace Pigs

Records on 65,536 Landrace pigs collected between 1985 and 1999 in herds on the National Swine Registry STAGES program were used to estimate additive genetic (animal), common environmental (litter), and residual variances and covariances for days to 250 lb, backfat, loin eye area, and lean growth rate. Analysis was by the REMLf90 program of I. Misztal using a multiple-trait animal model with fixed effects of contemporary group and sex and random effects of animal, litter, and residual error. Heritability estimates were 0.54, 0.39, 0.49, and 0.48 for days to 250 lb, backfat, loin eye area, and lean growth rate, respectively. Genetic correlations for lean growth rate with days to 250, backfat, and loin eye area were -0.83, -0.38, and 0.40, respectively. Phenotypic correlations for lean growth rate with days to 250 lb, backfat, and loin eye area were -0.80, -0.40, and 0.56, respectively. Litter effects were large only for days to 250 lb. Lean growth rate should be used as an important selection criteria in genetic improvement in pigs

Polariton Squeezing in Semiconductor Microcavities

We report squeezed polariton generation using parametric polariton four-wave mixing in semiconductor microcavities in the strong coupling regime. The geometry of the experiment corresponds to degenerate four-wave mixing, which gives rise to a bistability threshold. Spatial effects in the nonlinear regime are evidenced, and spatial filtering is required in order to optimize the measured squeezing. By measuring the noise of the outgoing light, we infer a 9 percent squeezing on the polariton field close to the bistability turning point

Comparison of Grain Sources (Barley, White Corn, and Yellow Corn) for Swine Diets and Their Effect on Production and Carcass Traits

Energy sources differ in content, quality, and availability of nutrients. The objective of this study was to identify and compare differences in production and carcass traits in pigs fed different energy sources. While pigs fed a barley-based diet had a smaller loin muscle area, there was no difference among diets when comparing fat depth or percent fat-free lean. Barley based-diets and a diet containing one-third yellow corn and two-thirds white corn had a lower lean gain per day on test. There was no significant difference in average daily gain or feed-to-gain ratios

The Effects of Long Term Selection for Reduced Backfat and Increased Loin Muscle Area on Meat and Eating Quality Traits in Duroc Swine

A study was conducted to evaluate differences in performance, carcass composition, and eating quality characteristics of pigs sired by purebred Duroc boars currently available and pigs sired by purebred Duroc boars from the mid 1980’s. Two lines were developed by splitting and randomly allocating littermate and ½ sib pairs of females to matings by current (CTP) or old (OTP) time period boars. Subsequent boar, barrow, and gilt progeny from two replications were weighed on test at a group mean live weight of 140 lbs. Off-test ultrasonic LMA, BF10, and IMF measurements were collected on 789 pigs at a mean live weight of 240 lb. Records on pigs sired by CTP boars, from both replications (n=556), represented 23 sires while pigs sired by OTP boars (n=231) consisted of 15 sire groups. All available barrows and randomly selected gilts (n=277) were sent to a commercial abattoir and measurements of tenth-rib backfat (CBF10), last rib backfat (CLRBF), last lumbar backfat (CLLBF), and loin muscle area (CLMA) were collected. Chemical intramuscular fat percentage was determined by lab analysis of a loin sample from the 10th rib face of the longissimus muscle. Additional meat and eating quality traits measured were: Minolta reflectance and Hunter L (24 and 48 h); pH (24 h and 7 d); water holding capacity; subjective visual scores for color, marbling, and firmness (48 h); Instron tenderness, cooking loss, and trained sensory panel evaluations (7 d). Six serial ultrasonic measurements of 10th rib loin muscle area (LMA), off-midline backfat (BF10), and intramuscular fat percentage (IMF) from the first replication were collected every two weeks and used to assess deposition rate and growth pattern differences. There was no significant difference in average daily gain of pigs sired by boars from the two time periods. Pigs sired by CTP boars had larger (P \u3c 0.05) LMA measurements and less BF10, while pigs sired by OTP had significantly more IMF. Carcass evaluation revealed larger CLMA measurements, and significantly less CBF10, CLRBF, and CLLBF measurements for pigs sired by CTP boars. Pigs sired by OTP boars had a higher intramuscular fat percentage, lower Instron tenderness values, and higher subjective marbling and color scores than pigs sired by CTP boars (P \u3c 0.05). There were no significant differences between time periods for Minolta reflectance, Hunter L (24 and 48 h), water holding capacity, pH (24 h and 7 d), and subjective firmness scores. Trained sensory evaluations revealed higher (P \u3c 0.05) flavor scores and lower off-flavor scores for OTP sired pigs; however, no significant differences in tenderness score, juiciness score, chewiness score, and cooking loss were found between lines. Progeny of OTP boars began the test period at heavier weights and begin to decrease in daily body weight gain toward the conclusion of the test period, finishing with no advantage in body weight when compared to progeny of CTP boars. The analysis of serial backfat measurements revealed a linear pattern of backfat deposition between 150 and 270 lbs. Pigs sired by OTP boars deposited more backfat (

Evaluation of p53 protein expression as a marker for long-term prognosis in colorectal carcinoma.

Mutation of the p53 gene is reported to be of prognostic importance in colorectal carcinomas. Immunohistochemical staining of the accumulated p53 gene product may be a simple alternative for p53 mutation analysis. Previous studies addressing the prognostic importance of p53 expression, however, yielded contradictory results. Therefore, we evaluated the importance of p53 expression as a marker for long-term prognosis in a well-characterised study population of 109 colorectal carcinomas. After antigen retrieval with target unmasking fluid (TUF), immunostaining of p53 was performed with both monoclonal antibody DO7 and polyclonal antibody CM1. Objective quantification of the p53 signal was assessed by a computerised image analyser. p53 expression was higher in non-mucinous tumours than in mucinous tumours (p53 labelling index = 30% and 17% respectively, P = 0.05), and in metastatic tumours compared with non-metastatic tumours (p53 labelling index = 37% and 22% respectively, P = 0.05). Other histopathological features were not related to p53 expression. In multivariate analysis, Dukes' stage (P = 0.02) and histological grade (P = 0.05) stood out as independent markers for prognosis. p53 expression was not an independent marker for prognosis. At present, p53 expression is not a useful marker for long-term prognosis. Further insight into the relationship between p53 mutations and p53 expression is needed to elucidate more precisely the clinical relevance of p53 alterations

Catechol-O-methyltransferase inhibition with tolcapone reduces the "wearing off" phenomenon and levodopa requirements in fluctuating parkinsonian patients.

BACKGROUND: More than 50% of patients with Parkinson's disease develop motor response fluctuations (the "wearing off" phenomenon) after more than five years of levodopa therapy. Inhibition of catechol-O-methyltransferase by tolcapone has been shown to increase levodopa bioavailability and plasma elimination half life, thereby prolonging the efficacy of levodopa. OBJECTIVES: The primary objective was to evaluate the efficacy of tolcapone in reducing "wearing off" in levodopa treated, fluctuating parkinsonian patients. Secondary objectives included assessment of reduction in levodopa requirements, improvement in patients' clinical status, duration of improvements, and tolerability of tolcapone. METHODS: In this multicentre, randomised, double blind, placebo controlled trial, 58 patients received placebo, 60 received 100 mg tolcapone three times daily (tid), and 59 received 200 mg tolcapone tid, in addition to levodopa/benserazide. RESULTS: After three months with 200 mg tolcapone tid, "off" time decreased by 26.2% of the baseline value, "on" time increased by 20.6% (P<O.01 v placebo), and the mean total daily levodopa dose decreased by 122 mg from the baseline dose of 676 mg (P<0.01). These responses were maintained up to nine months. With 100 mg tolcapone tid, "off" time decreased by 31.5% (P<0.05), "on" time increased by 21.3% (P<0.01), and the mean total daily levodopa dose decreased by 109 mg from the baseline dose of 668 mg (P<0.05). With 200 mg tolcapone tid, unified Parkinson's disease rating scale motor and total scores were significantly reduced, and quality of life (sickness impact profile) scores were significantly improved. Both dosages were well tolerated. Dyskinesia was the most often reported levodopa induced adverse event. Diarrhea was the most often reported non-dopaminergic adverse event and the most frequent reason for withdrawal from the study: four patients in the 100 mg tolcapone tid group and six in the 200 mg tid group withdrew because of diarrhea. CONCLUSION: Tolcapone prolongs "on" time in fluctuating parkinsonian patients while allowing a reduction in daily levodopa dosage, thereby improving the efficacy of long term levodopa therapy

Shank Modulates Postsynaptic Wnt Signaling to Regulate Synaptic Development

Prosap/Shank scaffolding proteins regulate the formation, organization, and plasticity of excitatory synapses. Mutations in SHANK family genes are implicated in autism spectrum disorder and other neuropsychiatric conditions. However, the molecular mechanisms underlying Shank function are not fully understood, and no study to date has examined the consequences of complete loss of all Shank proteins in vivo. Here we characterize the single Drosophila Prosap/Shank family homolog. Shank is enriched at the postsynaptic membrane of glutamatergic neuromuscular junctions and controls multiple parameters of synapse biology in a dose-dependent manner. Both loss and overexpression of Shank result in defects in synaptic bouton number and maturation. We find that Shank regulates a noncanonical Wnt signaling pathway in the postsynaptic cell by modulating the internalization of the Wnt receptor Fz2. This study identifies Shank as a key component of synaptic Wnt signaling, defining a novel mechanism for how Shank contributes to synapse maturation during neuronal development.Keywords: postsynaptic scaffold, Shank, synaptic development, Wnt signalingNational Institutes of Health (U.S.) (Grant MH097680)Massachusetts Institute of Technology. Simons Center for the Social Brai