110 research outputs found
The Establishment of the European Company: The First Cases from an Industrial Relations Perspective
Lactic acid production from lime-treated wheat straw by Bacillus coagulans: neutralization of acid by fed-batch addition of alkaline substrate
Conventional processes for lignocellulose-to-organic acid conversion requires pretreatment, enzymatic hydrolysis, and microbial fermentation. In this study, lime-treated wheat straw was hydrolyzed and fermented simultaneously to lactic acid by an enzyme preparation and Bacillus coagulans DSM 2314. Decrease in pH because of lactic acid formation was partially adjusted by automatic addition of the alkaline substrate. After 55 h of incubation, the polymeric glucan, xylan, and arabinan present in the lime-treated straw were hydrolyzed for 55%, 75%, and 80%, respectively. Lactic acid (40.7 g/l) indicated a fermentation efficiency of 81% and a chiral l(+)-lactic acid purity of 97.2%. In total, 711 g lactic acid was produced out of 2,706 g lime-treated straw, representing 43% of the overall theoretical maximum yield. Approximately half of the lactic acid produced was neutralized by fed-batch feeding of lime-treated straw, whereas the remaining half was neutralized during the batch phase with a Ca(OH)2 suspension. Of the lime added during the pretreatment of straw, 61% was used for the neutralization of lactic acid. This is the first demonstration of a process having a combined alkaline pretreatment of lignocellulosic biomass and pH control in fermentation resulting in a significant saving of lime consumption and avoiding the necessity to recycle lime
Identification of emulsifier potato peptides by bioinformatics: application to omega-3 delivery emulsions and release from potato industry side streams
We are grateful for the financial support from Innovation Fund Denmark (Grant nr: 7045-00021B, PROVIDE project). We also acknowledge K.M.C. amba (Brande, Denmark) and A.K.V. amba (Langholt, Denmark) for providing the potato samples used in this study.In this work, we developed a novel approach combining bioinformatics, testing of functionality and bottom-up proteomics to obtain peptide emulsifiers from potato side-streams. This is a significant advancement in the process to obtain emulsifier peptides and it is applicable to any type of protein. Our results indicated that structure at the interface is the major determining factor of the emulsifying activity of peptide emulsifiers. Fish oil-in-water emulsions with high physical stability were stabilized with peptides to be predicted to have facial amphiphilicity: (i) peptides with predominantly α-helix conformation at the interface and having 18–29 amino acids, and (ii) peptides with predominantly β-strand conformation at the interface and having 13–15 amino acids. In addition, high physically stable emulsions were obtained with peptides that were predicted to have axial hydrophobic/hydrophilic regions. Peptides containing the sequence FCLKVGV showed high in vitro antioxidant activity and led to emulsions with high oxidative stability. Peptide-level proteomics data and sequence analysis revealed the feasibility to obtain the potent emulsifier peptides found in this study (e.g. γ-1) by trypsin-based hydrolysis of different side streams in the potato industry.Innovation Fund Denmark
7045-00021
Effects of pretreatments of Napier Grass with deionized water, sulfuric acid and sodium hydroxide on pyrolysis oil characteristics
The depletion of fossil fuel reserves has led to
increasing interest in liquid bio-fuel from renewable biomass. Biomass is a complex organic material consisting of
different degrees of cellulose, hemicellulose, lignin,
extractives and minerals. Some of the mineral elements
tend to retard conversions, yield and selectivity during
pyrolysis processing. This study is focused on the extraction of mineral retardants from Napier grass using deionized water, dilute sodium hydroxide and sulfuric acid and subsequent pyrolysis in a fixed bed reactor. The raw biomass was characterized before and after each pretreatment
following standard procedure. Pyrolysis study was conducted
in a fixed bed reactor at 600 o�C, 30 �C/min and 30 mL/min N2 flow. Pyrolysis oil (bio-oil) collected was analyzed using standard analytic techniques. The bio-oil yield and characteristics from each pretreated sample were compared with oil from the non-pretreated sample. Bio-oil
yield from the raw sample was 32.06 wt% compared to
38.71, 33.28 and 29.27 wt% oil yield recorded from the
sample pretreated with sulfuric acid, deionized water and
sodium hydroxide respectively. GC–MS analysis of the oil
samples revealed that the oil from all the pretreated biomass had more value added chemicals and less ketones and
aldehydes. Pretreatment with neutral solvent generated
valuable leachate, showed significant impact on the ash
extraction, pyrolysis oil yield, and its composition and
therefore can be regarded as more appropriate for thermochemical conversion of Napier grass
Should patients with hip joint prosthesis receive antibiotic prophylaxis before dental treatment?
The safety committee of the American Academy of Orthopedic Surgeons (AAOS) recommended in 2009 that clinicians should consider antibiotic prophylaxis for all patients with total joint replacement before any invasive procedure that may cause bacteremia. This has aroused confusion and anger among dentists asking for the evidence. The present review deals with different aspects of the rationale for this recommendation giving attention to views both in favor of and against it
Scots pine needles macronutrient (N, P, K, CA, MG, and S) supply at different reclaimed mine soil substrates—as an indicator of the stability of developed forest ecosystems
Vier scenario's voor de toekomst van de medezeggenschap
Frank Tros is schrijver en specifiek verantwoordelijk voor hoofdstuk over Flexibilisering van de arbeidsmarkt en de gevolgen voor medezeggenschap, pp. 184-198. Het boek bevat een omgevingsverkenning van factoren die invloed hebben op de inrichting en functioneren van interne medezeggenschap op bedrijfsniveau in Nederlandse bedrijven. Op basis hiervan worden vier trendscenario's ontwikkeld voor de toekomst van de medezeggenschap. Het rapport is bedoeld om de discussie over de toekomst van medezeggenschap, waaronder specifiek over de rol van ondernemingsraden, te stimuleren. Frank Tros heeft zich daarbij geconcentreerd op (en hoofdstuk geschreven over) de gevolgen van de flexibilisering van de arbeidsmarkt en arbeidsorganisaties op de medezeggenschap
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