Final report for secure camera capture system (April 2016)

This report provides an overview of the Secure Camera Capture system produced by Team 6 in Spring 2016�s Senior Design 2 class. The project encompassed a three-part hardware and software system, used to capture, securely store, and retrieve photographs taken by an internet-equipped camera on a sixty-second timer. In the report, the project is outlined in both technical and nontechnical terms, the AES encryption method used, the Raspberry Pi microcontroller hardware, and the software used to tie them together. Specifications (both hardware and software) and how they were addressed are detailed, and a guide is provided to reconstruct the project using provided code. Finally, it provides the team�s perspective on the final product and possible improvements that could be made on the design

Cumulative live birth rates in low-prognosis women

No external funds were obtained for the present study. The OPTIMIST study was funded by The Netherlands Organization for Health Research and Development (ZonMW number 171102020).Peer reviewedPublisher PD

Synthesis of poly(3-hydroxyalkanoates) by mutant and recombinant Pseudomonas strains

We have studied the accumulation kinetics and physical characteristics of the poly(3-hydroxyalkanoates) (PHAs) formed by several Pseudomonas strains, mutants and recombinants. Although PHA synthesis generally begins only after an essential nutrient such as N, P, S or Mg becomes limiting, we have identified at least one strain (P. putida KT2442) that begins producing PHA during the exponential growth phase. This PHA is chemically and physically identical to that produced by P. oleovorans GPol, the strain in which we first identified PHA. Analysis of the PHA formed by a mutant strain defective in PHA degradation (P. oleovorans GPo500) revealed that the molecular mass (M(w)), the monomer composition and thermal characteristics were similar to that of the PHA of the wild-type parent strain P. oleovorans GPol. The pha locus of P. oleovorans encodes enzymes that are involved in PHA biosynthesis and degradation. It has been subcloned to study the two PHA polymerases separately in a PHA- mutant (GPp104) derived from P. putida KT2442. The recombinant strains accumulated lower PHA levels than the wild-type strains, and the M(w) of these polymers were lower than those produced by the wild-type P. oleovorans and parent strain. The monomer composition of the two PHAs formed by the two PHA polymerases differed, indicating that the PHA polymerases have different substrate specificities for the incorporation of 3-hydroxyoctanoate and 3-hydroxyhexanoate monomers into PHA. Despite these differences, the PHAs formed were essentially indistinguishable from wild-type PHAs with respect to their thermal characteristics