Structural validation of oral mucosal tissue using optical coherence tomography

Background: Optical coherence tomography (OCT) is a non-invasive optical technology using near-infrared light to produce cross-sectional tissue images with lateral resolution. Objectives: The overall aims of this study was to generate a bank of normative and pathological OCT data of the oral tissues to allow identification of cellular structures of normal and pathological processes with the aim to create a diagnostic algorithm which can be used in the early detection of oral disorders. Material and methods: Seventy-three patients with 78 suspicious oral lesions were referred for further management to the UCLH Head and Neck Centre, London. The entire cohort had their lesions surgically biopsied (incisional or excisional). The immediate ex vivo phase involved scanning the specimens using optical coherence tomography. The specimens were then processed by a histopathologist. Five tissue structures were evaluated as part of this study, including: keratin cell layer, epithelial layer, basement membrane, lamina propria and other microanatomical structures. Two independent assessors (clinician and pathologist trained to use OCT) assessed the OCT images and were asked to comment on the cellular structures and changes involving the five tissue structures in non-blind fashion. Results: Correct identification of the keratin cell layer and its structural changes was achieved in 87% of the cohort; for the epithelial layer it reached 93.5%, and 94% for the basement membrane. Microanatomical structures identification was 64% for blood vessels, 58% for salivary gland ducts and 89% for rete pegs. The agreement was “good” between the clinician and the pathologist. OCT was able to differential normal from pathological tissue and pathological tissue of different entities in this immediate ex vivo study. Unfortunately, OCT provided inadequate cellular and subcellular information to enable the grading of oral premalignant disorders. Conclusion: This study enabled the creation of OCT bank of normal and pathological oral tissues. The pathological changes identified using OCT enabled differentiation between normal and pathological tissues, and identification of different tissue pathologies. Further studies are required to assess the accuracy of OCT in identification of various pathological processes involving the oral tissues

Customers as decision-makers: strategic environmental assessment in the private sector

Despite its diversification and global spread, strategic environmental assessment (SEA) remains limited mainly to activities characterised by well-defined planning processes, typically within the public sector. This article explores the possible application of SEA within certain private-sector contexts where higher-level strategy-making itself is inherently weaker and development is often piecemeal and reactive. The possible adaptation of SEA to the preparation of a strategic document by a particular industrial concern in the UK is examined: this draws attention to the multi-actor nature of development processes within the industry. This leads to the suggestion that SEA in this setting should be thought of as a form of environmental advocacy oriented towards industrial customers, who are understood as sharing a decision-making role in infrastructure development.</p

Recognition of Familiar and Unfamiliar Melodies in Normal Aging and Alzheimers-Disease

We tested normal young and elderly adults and elderly Alzheimer\u27s disease (AD) patients on recognition memory for tunes. In Experiment 1, AD patients and age-matched controls received a study list and an old/new recognition test of highly familiar, traditional tunes, followed by a study list and test of novel tunes. The controls performed better than did the AD patients. The controls showed the \u27\u27mirror effect\u27\u27 of increased hits and reduced false alarms for traditional versus novel tunes, whereas the patients false-alarmed as often to traditional tunes as to novel tunes. Experiment 2 compared young adults and healthy elderly persons using a similar design. Performance was lower in the elderly group, but both younger and older subjects showed the mirror effect. Experiment 3 produced confusion between preexperimental familiarity and intraexperimental familiarity by mixing traditional and novel tunes in the study lists and tests. Here, the subjects in both age groups resembled the patients of Experiment 1 in failing to show the mirror effect. Older subjects again performed more poorly, and they differed qualitatively from younger subjects in setting stricter criteria for more nameable tunes. Distinguishing different sources of global familiarity is a factor in tune recognition, and the data suggest that this type of source monitoring is impaired in AD and involves different strategies in younger and older adults

Perception of Mode, Rhythm, and Contour in Unfamiliar Melodies: Effects of Age and Experience

We explored the ability of older (60-80 years old) and younger (18-23 years old) musicians and nonmusicians to judge the similarity of transposed melodies varying on rhythm, mode, and/or contour (Experiment 1) and to discriminate among melodies differing only in rhythm, mode, or contour (Experiment 2). Similarity ratings did not vary greatly among groups, with tunes differing only by mode being rated as most similar. In the same/different discrimination task, musicians performed better than nonmusicians, but we found no age differences. We also found that discrimination of major from minor tunes was difficult for everyone, even for musicians. Mode is apparently a subtle dimension in music, despite its deliberate use in composition and despite people\u27s ability to label minor as sad and major as happy

Metabolic and cardiac adaptation to chronic pharmacologic blockade of facilitative glucose transport in murine dilated cardiomyopathy and myocardial ischemia

Abstract GLUT transgenic and knockout mice have provided valuable insight into the role of facilitative glucose transporters (GLUTs) in cardiovascular and metabolic disease, but compensatory physiological changes can hinder interpretation of these models. To determine whether adaptations occur in response to GLUT inhibition in the failing adult heart, we chronically treated TG9 mice, a transgenic model of dilated cardiomyopathy and heart failure, with the GLUT inhibitor ritonavir. Glucose tolerance was significantly improved with chronic treatment and correlated with decreased adipose tissue retinol binding protein 4 (RBP4) and resistin. A modest improvement in lifespan was associated with decreased cardiomyocyte brain natriuretic peptide (BNP) expression, a marker of heart failure severity. GLUT1 and −12 protein expression was significantly increased in left ventricular (LV) myocardium in ritonavir-treated animals. Supporting a switch from fatty acid to glucose utilization in these tissues, fatty acid transporter CD36 and fatty acid transcriptional regulator peroxisome proliferator-activated receptor α (PPARα) mRNA were also decreased in LV and soleus muscle. Chronic ritonavir also increased cardiac output and dV/dt-d in C57Bl/6 mice following ischemia-reperfusion injury. Taken together, these data demonstrate compensatory metabolic adaptation in response to chronic GLUT blockade as a means to evade deleterious changes in the failing heart

Spin squeezing of high-spin, spatially extended quantum fields

Investigations of spin squeezing in ensembles of quantum particles have been limited primarily to a subspace of spin fluctuations and a single spatial mode in high-spin and spatially extended ensembles. Here, we show that a wider range of spin-squeezing is attainable in ensembles of high-spin atoms, characterized by sub-quantum-limited fluctuations in several independent planes of spin-fluctuation observables. Further, considering the quantum dynamics of an $f=1$ ferromagnetic spinor Bose-Einstein condensate, we demonstrate theoretically that a high degree of spin squeezing is attained in multiple spatial modes of a spatially extended quantum field, and that such squeezing can be extracted from spatially resolved measurements of magnetization and nematicity, i.e.\ the vector and quadrupole magnetic moments, of the quantum gas. Taking into account several experimental limitations, we predict that the variance of the atomic magnetization and nematicity may be reduced as far as 20 dB below the standard quantum limits.Comment: 18 pages, 5 figure

Analysis of margin classification systems for assessing the risk of local recurrence after soft tissue sarcoma resection

Purpose: To compare the ability of margin classification systems to determine local recurrence (LR) risk after soft tissue sarcoma (STS) resection. Methods: Two thousand two hundred seventeen patients with nonmetastatic extremity and truncal STS treated with surgical resection and multidisciplinary consideration of perioperative radiotherapy were retrospectively reviewed. Margins were coded by residual tumor (R) classification (in which microscopic tumor at inked margin defines R1), the R+1mm classification (in which microscopic tumor within 1 mm of ink defines R1), and the Toronto Margin Context Classification (TMCC; in which positive margins are separated into planned close but positive at critical structures, positive after whoops re-excision, and inadvertent positive margins). Multivariate competing risk regression models were created. Results: By R classification, LR rates at 10-year follow-up were 8%, 21%, and 44% in R0, R1, and R2, respectively. R+1mm classification resulted in increased R1 margins (726 v 278, P &lt; .001), but led to decreased LR for R1 margins without changing R0 LR; for R0, the 10-year LR rate was 8% (range, 7% to 10%); for R1, the 10-year LR rate was 12% (10% to 15%) . The TMCC also showed various LR rates among its tiers (P &lt; .001). LR rates for positive margins on critical structures were not different from R0 at 10 years (11% v 8%, P = .18), whereas inadvertent positive margins had high LR (5-year, 28% [95% CI, 19% to 37%]; 10-year, 35% [95% CI, 25% to 46%]; P &lt; .001). Conclusion: The R classification identified three distinct risk levels for LR in STS. An R+1mm classification reduced LR differences between R1 and R0, suggesting that a negative but &lt; 1-mm margin may be adequate with multidisciplinary treatment. The TMCC provides additional stratification of positive margins that may aid in surgical planning and patient education

Temperature Effects on Threshold Counterion Concentration to Induce Aggregation of fd Virus

We seek to determine the mechanism of like-charge attraction by measuring the temperature dependence of critical divalent counterion concentration ($\rm{C_{c}}$) for the aggregation of fd viruses. We find that an increase in temperature causes $\rm{C_c}$ to decrease, primarily due to a decrease in the dielectric constant ($\epsilon$) of the solvent. At a constant $\epsilon$, $\rm{C_c}$ is found to increase as the temperature increases. The effects of $T$ and $\epsilon$ on $\rm {C_{c}}$ can be combined to that of one parameter: Bjerrum length ($l_{B}$). $\rm{C_{c}}$ decreases exponentially as $l_{B}$ increases, suggesting that entropic effect of counterions plays an important role at the onset of bundle formation.Comment: 12 pages, 3 figure

Protocols for optimal readout of qubits using a continuous quantum nondemolition measurement

We study how the spontaneous relaxation of a qubit affects a continuous quantum non-demolition measurement of the initial state of the qubit. Given some noisy measurement record $\Psi$, we seek an estimate of whether the qubit was initially in the ground or excited state. We investigate four different measurement protocols, three of which use a linear filter (with different weighting factors) and a fourth which uses a full non-linear filter that gives the theoretically optimal estimate of the initial state of the qubit. We find that relaxation of the qubit at rate $1/T_1$ strongly influences the fidelity of any measurement protocol. To avoid errors due to this decay, the measurement must be completed in a time that decrease linearly with the desired fidelity while maintaining an adequate signal to noise ratio. We find that for the non-linear filter the predicted fidelity, as expected, is always better than the linear filters and that the fidelity is a monotone increasing function of the measurement time. For example, to achieve a fidelity of 90%, the box car linear filter requires a signal to noise ratio of $\sim 30$ in a time $T_1$ whereas the non-linear filter only requires a signal to noise ratio of $\sim 18$.Comment: 12 pages, 6 figure

Jump-like unravelings for non-Markovian open quantum systems

Non-Markovian evolution of an open quantum system can be `unraveled' into pure state trajectories generated by a non-Markovian stochastic (diffusive) Schr\"odinger equation, as introduced by Di\'osi, Gisin, and Strunz. Recently we have shown that such equations can be derived using the modal (hidden variable) interpretation of quantum mechanics. In this paper we generalize this theory to treat jump-like unravelings. To illustrate the jump-like behavior we consider a simple system: A classically driven (at Rabi frequency $\Omega$) two-level atom coupled linearly to a three mode optical bath, with a central frequency equal to the frequency of the atom, $\omega_0$, and the two side bands have frequencies $\omega_0\pm\Omega$. In the large $\Omega$ limit we observed that the jump-like behavior is similar to that observed in this system with a Markovian (broad band) bath. This is expected as in the Markovian limit the fluorescence spectrum for a strongly driven two level atom takes the form of a Mollow triplet. However the length of time for which the Markovian-like behaviour persists depends upon {\em which} jump-like unraveling is used.Comment: 11 pages, 5 figure