Actinoplanes siamensis sp. nov., isolated from soil

A Gram-positive filamentous bacterial strain that developed large campanulate sporangia at the ends of sporangiophores on substrate mycelium was isolated from bamboo forest soil in Thailand. According to the results of a polyphasic taxonomic study, our isolate had typical characteristics of members of the genus Actinoplanes. The 16S rRNA gene sequence analysis also indicated that strain A-T 6646<sup>T</sup> belonged to the genus Actinoplanes, being most closely related to Actinoplanes liguriensis DSM 43865<sup>T</sup> (97.61 %) and Actinoplanes octamycinicus NBRC 14524<sup>T</sup> (97.52 %). The DNA-DNA relatedness values, which differentiate the new strain from the most closely related species, were significantly below 70 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained xylose and arabinose. The predominant menaquinone was MK-9(H<sub>4</sub>). The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant cellular fatty acids were iso-C<sub>16: 0</sub>, anteiso-C<sub>17: 0</sub>, iso-C<sub>15: 0</sub> and anteiso-C<sub>15: 0</sub>. Following an evaluation of phenotypic, chemotaxonomic and genotypic studies, the isolate is proposed to represent a novel species to be named Actinoplanes siamensis sp. nov. The type strain is A-T 6646<sup>T</sup> (= BCC 46194<sup>T</sup> = NBRC 109076<sup>T</sup>). 2013 IUM

Planobispora takensis sp. nov., isolated from soil

A novel Gram-stain-positive, filamentous bacterial strain (A-T 7458<sup>T</sup>) was isolated from soil collected from hill evergreen forest in Thailand. The strain developed cylindrical sporangia containing a longitudinal pair of motile spores, on short ramifications of aerial mycelia. From a taxonomic study using a polyphasic approach, strain A-T 7458<sup>T</sup>had typical characteristics of members of the genus Planobispora. 16S rRNA gene sequence analysis indicated that the isolate was closely related to Planobispora siamensis A-T 4600<sup>T</sup>(98.5 %), Planobispora rosea JCM 3166<sup>T</sup>(97.6 %) and Planobispora longispora NBRC 13918<sup>T</sup>(97.6 %). The DNA-DNA relatedness values, which differentiated the novel strain from the closest species, were significantly below 70 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, madurose, mannose and glucose. The predominant menaquinone was MK-9(H<sub>2</sub>). The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside and aminophosphoglycolipid. The predominant cellular fatty acids were unsaturated C<sub>17:1</sub> and C<sub>18:1</sub>, and saturated C<sub>16:0</sub> and C<sub>17:0</sub>. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the novel isolate is proposed to represent a novel species of the genus Planobispora, to be named Planobispora takensis sp. nov. The type strain is A-T 7458<sup>T</sup>(=BCC 48396<sup>T</sup>=NBRC 109077<sup>T</sup>). 2014 IUM