Antioxidant defense system participation in cross-adaptation of Ocimum basilicum to complex action of UV-B and salinity

The 10 min UV-B irradiation (12 dj/m2) and 100mM NaCl action on medical plants Ocimum basilicum L., grown in water culture, was studying. The plants treated by UV-B irradiation were more resistent to salinity. There were two groups of compounds. One - proline, carotinoids, phenols are accumulated under salinity conditions. Second group - pigments - antocyans and flavones. Its biosynthesis is stimulated by UV-B irradiation. Under complex stressor action pigments were increased and this increasing helped plants to overcome the high salinity. The antioxidant enzyme superoxid dismutase did not play essential role in adaptive response. The low molecular weight antioxidants were more effective for formation of adaptation response. The peroxidase activity had not correlation with superoxid dismutase activity and we supposed that H2O2 could be result of other process and served as signal molecul

Evaluation of antioxidatant potential in medical plants under UV-B-irradiation

The UV-B irradiation action (6 -36,9 dg/m2) on medical plants Artemisia lerchiana L., Nigella sativa L., Ocimum basilicum L., grown in water culture, was studying. In plant leaves and roots the content of malondialdehyde, pigments, phenols, flavonoids, proline, and activities of antioxidant enzymes were determined. Investigated plants were able to resist to action of UV-B-irradiation (low and middle dose) without any power input for increasing of biosynthesis of low and high molecular component of antioxidant defense system. The damage level under stress conditions was low. At first hours plants used the constitutive level of antioxidants and after 24 h it was needed the antioxidant pool refilling. Carotinoids, flavonoids and proline were used by plants in 4 h after irradiation, the antocian synthesis increased after 12 h