Phenotypically determined resistance of Neisseria gonorrhoeae to normal human serum: environmental factors in subcutaneous chambers in guinea pigs

Some gonococci obtained from human urethral exudate or from subcutaneously implanted chambers in guinea pigs show a resistance to killing by human serum which is lost on subculture in vitro after a few generations. The environmental factors which may influence the phenotypic expression of resistance to serum killing were investigated in guinea pig chambers and in chamber fluid in vitro. The redox potential in chambers before and after infection was lower than that of heart blood but conditions were not anaerobic; H2O2 increased the redox potential but did not decrease gonococcal serum resistance. The chambers were slightly alkaline before and after infection. When the concentration of glucose (depleted in infected chambers by the abundant polymorphonuclear cells) was restored to excess, the serum resistance of the gonococci was unaffected. Concentrations of free amino acids in chambers changed little during infection. Gonococci adapted to growth in chambers and subsequently rendered serum-sensitive by growing once on agar reverted to serum-resistance after 0.5 to 1 h incubation in chamber fluid in vitro at 37°C but not at 25°C or 4°C. After 16 to 24 h growth at 37°C, resistance was again lost. The reversion to serum resistance did not occur in a complex laboratory medium. Examination of the chamber fluid after growth of gonococci in vitro showed depletion of lactate, glutamine and proline

Scaling of the superfluid density in superfluid films

We study scaling of the superfluid density with respect to the film thickness by simulating the $x-y$ model on films of size $L \times L \times H$ ($L >> H$) using the cluster Monte Carlo. While periodic boundary conditions where used in the planar ($L$) directions, Dirichlet boundary conditions where used along the film thickness. We find that our results can be scaled on a universal curve by introducing an effective thickness. In the limit of large $H$ our scaling relations reduce to the conventional scaling forms. Using the same idea we find scaling in the experimental results using the same value of $\nu = 0.6705$.Comment: 4 pages, one postscript file replaced by one Latex file and 5 postscript figure