SNX4 in Complex with Clathrin and Dynein: Implications for Endosome Movement

BACKGROUND:Sorting nexins (SNXs) constitute a family of proteins classified by their phosphatidylinositol (PI) binding Phox homology (PX) domain. Some members regulate intracellular trafficking. We have here investigated mechanisms underlying SNX4 mediated endosome to Golgi transport. METHODOLOGY/PRINCIPAL FINDINGS:We show that SNX4 forms complexes with clathrin and dynein. The interactions were inhibited by wortmannin, a PI3-kinase inhibitor, suggesting that they form when SNX4 is associated with PI(3)P on endosomes. We further localized the clathrin interacting site on SNX4 to a clathrin box variant. A short peptide containing this motif was sufficient to pull down both clathrin and dynein. Knockdown studies demonstrated that clathrin is not required for the SNX4/dynein interaction. Moreover, clathrin knockdown led to increased Golgi transport of the toxin ricin, as well as redistribution of endosomes. CONCLUSIONS/SIGNIFICANCE:We discuss the possibility of clathrin serving as a regulator of SNX4-dependent transport. Upon clathrin release, dynein may bind SNX4 and mediate retrograde movement

Grammatical gender and linguistic complexity I: General issues and specific studies

The many facets of grammatical gender remain one of the most fruitful areas of linguistic research, and pose fascinating questions about the origins and development of complexity in language. The present work is a two-volume collection of 13 chapters on the topic of grammatical gender seen through the prism of linguistic complexity. The contributions discuss what counts as complex and/or simple in grammatical gender systems, whether the distribution of gender systems across the world’s languages relates to the language ecology and social history of speech communities. Contributors demonstrate how the complexity of gender systems can be studied synchronically, both in individual languages and over large cross-linguistic samples, and diachronically, by exploring how gender systems change over time. In addition to three chapters on the theoretical foundations of gender complexity, volume one contains six chapters on grammatical gender and complexity in individual languages and language families of Africa, New Guinea, and South Asia. This volume is complemented by volume two, which consists of three chapters providing diachronic and typological case studies, followed by a final chapter discussing old and new theoretical and empirical challenges in the study of the dynamics of gender complexity

Grammatical gender and linguistic complexity I: General issues and specific studies

The many facets of grammatical gender remain one of the most fruitful areas of linguistic research, and pose fascinating questions about the origins and development of complexity in language. The present work is a two-volume collection of 13 chapters on the topic of grammatical gender seen through the prism of linguistic complexity. The contributions discuss what counts as complex and/or simple in grammatical gender systems, whether the distribution of gender systems across the world’s languages relates to the language ecology and social history of speech communities. Contributors demonstrate how the complexity of gender systems can be studied synchronically, both in individual languages and over large cross-linguistic samples, and diachronically, by exploring how gender systems change over time. In addition to three chapters on the theoretical foundations of gender complexity, volume one contains six chapters on grammatical gender and complexity in individual languages and language families of Africa, New Guinea, and South Asia. This volume is complemented by volume two, which consists of three chapters providing diachronic and typological case studies, followed by a final chapter discussing old and new theoretical and empirical challenges in the study of the dynamics of gender complexity

Grammatical gender and linguistic complexity I: General issues and specific studies

The many facets of grammatical gender remain one of the most fruitful areas of linguistic research, and pose fascinating questions about the origins and development of complexity in language. The present work is a two-volume collection of 13 chapters on the topic of grammatical gender seen through the prism of linguistic complexity. The contributions discuss what counts as complex and/or simple in grammatical gender systems, whether the distribution of gender systems across the world’s languages relates to the language ecology and social history of speech communities. Contributors demonstrate how the complexity of gender systems can be studied synchronically, both in individual languages and over large cross-linguistic samples, and diachronically, by exploring how gender systems change over time. In addition to three chapters on the theoretical foundations of gender complexity, volume one contains six chapters on grammatical gender and complexity in individual languages and language families of Africa, New Guinea, and South Asia. This volume is complemented by volume two, which consists of three chapters providing diachronic and typological case studies, followed by a final chapter discussing old and new theoretical and empirical challenges in the study of the dynamics of gender complexity

Grammatical gender and linguistic complexity I: General issues and specific studies

The many facets of grammatical gender remain one of the most fruitful areas of linguistic research, and pose fascinating questions about the origins and development of complexity in language. The present work is a two-volume collection of 13 chapters on the topic of grammatical gender seen through the prism of linguistic complexity. The contributions discuss what counts as complex and/or simple in grammatical gender systems, whether the distribution of gender systems across the world’s languages relates to the language ecology and social history of speech communities. Contributors demonstrate how the complexity of gender systems can be studied synchronically, both in individual languages and over large cross-linguistic samples, and diachronically, by exploring how gender systems change over time. In addition to three chapters on the theoretical foundations of gender complexity, volume one contains six chapters on grammatical gender and complexity in individual languages and language families of Africa, New Guinea, and South Asia. This volume is complemented by volume two, which consists of three chapters providing diachronic and typological case studies, followed by a final chapter discussing old and new theoretical and empirical challenges in the study of the dynamics of gender complexity

Grammatical gender and linguistic complexity I: General issues and specific studies

The many facets of grammatical gender remain one of the most fruitful areas of linguistic research, and pose fascinating questions about the origins and development of complexity in language. The present work is a two-volume collection of 13 chapters on the topic of grammatical gender seen through the prism of linguistic complexity. The contributions discuss what counts as complex and/or simple in grammatical gender systems, whether the distribution of gender systems across the world’s languages relates to the language ecology and social history of speech communities. Contributors demonstrate how the complexity of gender systems can be studied synchronically, both in individual languages and over large cross-linguistic samples, and diachronically, by exploring how gender systems change over time. In addition to three chapters on the theoretical foundations of gender complexity, volume one contains six chapters on grammatical gender and complexity in individual languages and language families of Africa, New Guinea, and South Asia. This volume is complemented by volume two, which consists of three chapters providing diachronic and typological case studies, followed by a final chapter discussing old and new theoretical and empirical challenges in the study of the dynamics of gender complexity

Grammatical gender and linguistic complexity I: General issues and specific studies

The many facets of grammatical gender remain one of the most fruitful areas of linguistic research, and pose fascinating questions about the origins and development of complexity in language. The present work is a two-volume collection of 13 chapters on the topic of grammatical gender seen through the prism of linguistic complexity. The contributions discuss what counts as complex and/or simple in grammatical gender systems, whether the distribution of gender systems across the world’s languages relates to the language ecology and social history of speech communities. Contributors demonstrate how the complexity of gender systems can be studied synchronically, both in individual languages and over large cross-linguistic samples, and diachronically, by exploring how gender systems change over time. In addition to three chapters on the theoretical foundations of gender complexity, volume one contains six chapters on grammatical gender and complexity in individual languages and language families of Africa, New Guinea, and South Asia. This volume is complemented by volume two, which consists of three chapters providing diachronic and typological case studies, followed by a final chapter discussing old and new theoretical and empirical challenges in the study of the dynamics of gender complexity

Grammatical gender and linguistic complexity I: General issues and specific studies

The many facets of grammatical gender remain one of the most fruitful areas of linguistic research, and pose fascinating questions about the origins and development of complexity in language. The present work is a two-volume collection of 13 chapters on the topic of grammatical gender seen through the prism of linguistic complexity. The contributions discuss what counts as complex and/or simple in grammatical gender systems, whether the distribution of gender systems across the world’s languages relates to the language ecology and social history of speech communities. Contributors demonstrate how the complexity of gender systems can be studied synchronically, both in individual languages and over large cross-linguistic samples, and diachronically, by exploring how gender systems change over time. In addition to three chapters on the theoretical foundations of gender complexity, volume one contains six chapters on grammatical gender and complexity in individual languages and language families of Africa, New Guinea, and South Asia. This volume is complemented by volume two, which consists of three chapters providing diachronic and typological case studies, followed by a final chapter discussing old and new theoretical and empirical challenges in the study of the dynamics of gender complexity

The OPERA experiment Target Tracker

The main task of the Target Tracker detector of the long baseline neutrino oscillation OPERA experiment is to locate in which of the target elementary constituents, the lead/emulsion bricks, the neutrino interactions have occurred and also to give calorimetric information about each event. The technology used consists in walls of two planes of plastic scintillator strips, one per transverse direction. Wavelength shifting fibres collect the light signal emitted by the scintillator strips and guide it to both ends where it is read by multi-anode photomultiplier tubes. All the elements used in the construction of this detector and its main characteristics are described.Comment: 25 pages, submitted to Nuclear Instrument and Method