Occurrence of Killer Yeast Strains in Fruit and Berry Wine Yeast Populations

Apple, cranberry, chokeberry and Lithuanian red grape wine yeast populations were used for the determination of killer yeast occurrence. According to the tests of the killer characteristics and immunity the isolated strains were divided into seven groups. In this work the activity of killer toxins purified from some typical strains was evaluated. The analysed strains produced different amounts of active killer toxin and some of them possessed new industrially significant killer properties. Total dsRNA extractions in 11 killer strains of yeast isolated from spontaneous fermentations revealed that the molecular basis of the killer phenomenon was not only dsRNAs, but also unidentified genetic determinants

Naujų apsaugos priemonių prieš sumedėjusių augalų grybinių ligų sukėlėjus pirminė analizė

The aim of this work was to determine the impact of bacterial isolates Tx and Ux (of two kinds) from spontaneous fruit-berry fermentation upon fungal disease agents from the genera Alternaria and Fusarium. The disease agents were isolated from various ornamental plants growing in the city greenery. The killer activity of bacterial isolates is determined by the ability of the test strains to form lysis zones on the lawns of the test a' S. cerevisiae strain and plant disease agents. S. cerevisiae standard K7, Rom-K100, M437, MS300 killer strains were used as a control. It has been previously demonstrated that the toxins produced by Tx and Ux bacterial isolates are able to destroy not only yeasts of the genus Saccharomyces but also of the genera Candida, Kluyveromyces as well as such plant disease agents as Verticillum albo-atrum and Venturia ineaqualis. Tests of the impact of these toxins upon fungi of the genera Alternaria and Fusarium revealed the highest killing activity during the intensive growing stage on the YEPD and MB media (pH 4.8) at a temperature of 20–30 °C. The obtained results could be employed while elaborating new and efficient plant protection measures

Naujų apsaugos priemonių prieš sumedėjusių augalų grybinių ligų sukėlėjus pirminė analizė

The aim of this work was to determine the impact of bacterial isolates Tx and Ux (of two kinds) from spontaneous fruit-berry fermentation upon fungal disease agents from the genera Alternaria and Fusarium. The disease agents were isolated from various ornamental plants growing in the city greenery. The killer activity of bacterial isolates is determined by the ability of the test strains to form lysis zones on the lawns of the test a' S. cerevisiae strain and plant disease agents. S. cerevisiae standard K7, Rom-K100, M437, MS300 killer strains were used as a control. It has been previously demonstrated that the toxins produced by Tx and Ux bacterial isolates are able to destroy not only yeasts of the genus Saccharomyces but also of the genera Candida, Kluyveromyces as well as such plant disease agents as Verticillum albo-atrum and Venturia ineaqualis. Tests of the impact of these toxins upon fungi of the genera Alternaria and Fusarium revealed the highest killing activity during the intensive growing stage on the YEPD and MB media (pH 4.8) at a temperature of 20–30 °C. The obtained results could be employed while elaborating new and efficient plant protection measures