Burlington, Vt. as a manufacturing, business, and commercial center : with brief sketches of its history, attractions, leading industries, and institutions.

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An immunochemical approach to the study of DNA damage and repair

The overall objective of this project has been to develop immunochemical methods to quantitate unique DNA base damages in order to facilitate studies on radiation-induced damage production and repair. Specifically, we have been using antibodies raised to damaged bases to quantitate unique lesions in model systems in order to evaluate their potential biological consequences. Our approach has been to synthesize modified nucleotides or nucleosides, conjugate them to protein carriers, and use the conjugates as immunogens in rabbits or to prepare monoclonal antibodies. We have been studying damages that are stable radiolysis products found in X-irradiated DNA and thus of potential biological consequence. Our aim is to build an in vitro and in vivo data base on the interactions between model DNA lesions and such cellular enzymes as DNA polymerases and repair endonucleases. Initial studies have focused on pyrimidine ring saturation products (thymine glycol.and dihydrothymine), products resulting from ring fragmentation or base loss (urea, {Beta}-ureidoisobutyric acid, abasic sites), 7-hydro-8-oxopurines, and more recently, cytosine radiolysis products. These modified bases serve as useful models for examining the potential lethal and/or mutagenic (carcinogenic) effects of the products of DNA radiolysis

Latviesu gramata un biblioteka: 1525-2000 Starptautiska konference. Materialu krajums

In 2 vols.Available from Latvian Academic Library / LAL - Latvian Academic LibrarySIGLEUnited Nations Educational, Scientific and Cultural Organization (UNESCO), Paris (France); Ministry of Culture of the Republic of Latvia, Riga (Latvia); Culture Capital Foundation, Riga (Latvia); Latvian Society of Riga, Riga (Latvia); Soros Foundation - Latvia, Riga (Latvia)2. rev. sup. ed.LVLatvi