3 research outputs found
Bacterial Colonization of LowâWettable Surfaces is Driven by Culture Conditions and Topography
Effect of surface lowâwettability on bacterial colonization has become a prominent subject for the development of antibacterial coatings. However, bacteria's fate on such surfaces immersed in liquid as well as causal factors is poorly understood. This question is addressed by using a range of coatings with increasing hydrophobicity, to superhydrophobic, obtained by an atmospheric plasma polymer method allowing series production. Chemistry, wettability, and topography are thoroughly described, as well as bacterial colonization by in situ live imaging up to 24 h culture time in different liquid media. In the extreme case of superhydrophobic coating, substrates are significantly less colonized in biomoleculeâpoor liquids and for shortâterm culture only. Complex statistical analysis demonstrates that bacterial colonization on these lowâwettable substrates is predominantly controlled by the culture conditions and only secondary by topographic coating's properties (variation in surface structuration with almost constant mean height). Wettability is less responsible for bacterial colonization reduction in these conditions, but allows the coatings to preserve colonizationâprevention properties in nutritive media when topography is masked by fouling. Even after longâterm culture in rich medium, many large places of the superhydrophobic coating are completely free of bacteria in relation to their capacity to preserve air trapping
Internalization efficiency of adsorbed particles
Internalization efficiency of adsorbed particle