4 research outputs found
Eggshell Membrane as a Biomaterial for Bone Regeneration
The physicochemical features of the avian eggshell membrane play an essential role in
the process of calcium carbonate deposition during shell mineralization, giving rise to a porous
mineralized tissue with remarkable mechanical properties and biological functions. The membrane
could be useful by itself or as a bi-dimensional scaffold to build future bone-regenerative materials.
This review focuses on the biological, physical, and mechanical properties of the eggshell membrane
that could be useful for that purpose. Due to its low cost and wide availability as a waste byproduct of
the egg processing industry, repurposing the eggshell membrane for bone bio-material manufacturing
fulfills the principles of a circular economy. In addition, eggshell membrane particles have has the
potential to be used as bio-ink for 3D printing of tailored implantable scaffolds. Herein, a literature
review was conducted to ascertain the degree to which the properties of the eggshell membrane
satisfy the requirements for the development of bone scaffolds. In principle, it is biocompatible and
non-cytotoxic, and induces proliferation and differentiation of different cell types. Moreover, when
implanted in animal models, it elicits a mild inflammatory response and displays characteristics
of stability and biodegradability. Furthermore, the eggshell membrane possesses a mechanical
viscoelastic behavior comparable to other collagen-based systems. Overall, the biological, physical,
and mechanical features of the eggshell membrane, which can be further tuned and improved, make
this natural polymer suitable as a basic component for developing new bone graft materialsinisterio de Ciencia de InnovaciĂłn (MCINN) (Ministry
of Science and Innovation, Spain) project PGC2018-102047-B-I00 (MCIU/AEI/FEDER/UE)FundaciĂłn
Mutua Madrileña, Grant/Award Number: FMM-AP17196-2019the Instituto de Salud Carlos
III, ERDF funds (DTS21/00098)the ConsejerĂa de EconomĂa y Universidad, Junta de AndalucĂa
(PYC20 RE 015 UGR; P20_00208 of 2020)European Regional Development Fund (ERDF)–
Next Generation/EU program and grant AYUD/2022/33525 (FICYT)Canadian Natural Sciences and Engineering Research Council (NSERC, Discovery
program: RGPIN-2022-04803)PID2022-142886NB-I00 funded
by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe,” by the “European
Union.”The Ministerio de Ciencia TecnologĂa e InnovaciĂłn (Ministry of Science, Technology, and
Innovation, Colombia) provided support for A.T-M´s participatio
Evaluation of Phenolic Compounds and Pigments Content in Yellow Bell Pepper Wastes
This research was supported by the Aula de Empresa "Vellsam-UGR para la Innovacion en Nutricion y Salud". Vito Verardo thanks the Spanish Ministry of Economy and Competitiveness (MINECO) for "Ramon y Cajal" contract (RYC-2015-18795).Bell peppers are one of the most important species consumed and cultivated in Spain.
Peppers are a source of carotenoids and phenolic compounds widely associated with biological
activities such as antimicrobial, antiseptic, anticancer, counterirritant, cardioprotective, appetite
stimulator, antioxidant, and immunomodulator. However, undersized and damaged fruits are usually
wasted. Thus, in order to evaluate the phenolic content, a Box–Behnken design has been carried out
to optimize the extraction from Capsicum annuum yellow pepper by ultrasound-assisted extraction
(UAE). The independent factors were time (min), ethanol/water (% v/v) and solvent/sample ratio
(v/w). The model was validated by ANOVA and confirmed. Furthermore, the whole pepper and
the pepper without peduncles and seeds were extracted using optimal conditions and characterized
by HPLC-ESI-TOF-MS. Moreover, their antioxidant activities, measured by three different methods
(DPPH, ABTS, and FRAP), carotenoid composition, assessed by HPLC-MS, and chlorophyll content,
assessed by a spectrophotometric method, were compared. A total of 38 polar compounds were
found of which seven have been identified in pepper fruit extracts for the first time. According to
the results, whole pepper (WP) samples presented higher content in phenolic acids; meanwhile, the
edible portion (EP) was higher in flavonoids. No differences were found in the antioxidant activity
except for the FRAP assay where the WP sample showed higher radical scavenging activity. EP
samples showed the highest content of carotenoids and WP ones in chlorophylls.Aula de Empresa "Vellsam-UGR para la Innovacion en Nutricion y Salud"Spanish Ministry of Economy and Competitiveness (MINECO) for "Ramon y Cajal" RYC-2015-1879
Current Status of the Diagnosis and Management of Osteoporosis
Osteoporosis has been defined as the silent disease of the 21st century, becoming a public health risk due to its severity, chronicity and progression and affecting mainly postmenopausal women and older adults. Osteoporosis is characterized by an imbalance between bone resorption and bone production. It is diagnosed through different methods such as bone densitometry and dual X-rays. The treatment of this pathology focuses on different aspects. On the one hand, pharmacological treatments are characterized by the use of anti-resorptive drugs, as well as emerging regenerative medicine treatments such as cell therapies and the use of bioactive hydrogels. On the other hand, non-pharmacological treatments are associated with lifestyle habits that should be incorporated, such as physical activity, diet and the cessation of harmful habits such as a high consumption of alcohol or smoking. This review seeks to provide an overview of the theoretical basis in relation to bone biology, the existing methods for diagnosis and the treatments of osteoporosis, including the development of new strategies
Evaluation of Phenolic Compounds and Pigments Content in Yellow Bell Pepper Wastes.
Bell peppers are one of the most important species consumed and cultivated in Spain. Peppers are a source of carotenoids and phenolic compounds widely associated with biological activities such as antimicrobial, antiseptic, anticancer, counterirritant, cardioprotective, appetite stimulator, antioxidant, and immunomodulator. However, undersized and damaged fruits are usually wasted. Thus, in order to evaluate the phenolic content, a Box-Behnken design has been carried out to optimize the extraction from Capsicum annuum yellow pepper by ultrasound-assisted extraction (UAE). The independent factors were time (min), ethanol/water (% v/v) and solvent/sample ratio (v/w). The model was validated by ANOVA and confirmed. Furthermore, the whole pepper and the pepper without peduncles and seeds were extracted using optimal conditions and characterized by HPLC-ESI-TOF-MS. Moreover, their antioxidant activities, measured by three different methods (DPPH, ABTS, and FRAP), carotenoid composition, assessed by HPLC-MS, and chlorophyll content, assessed by a spectrophotometric method, were compared. A total of 38 polar compounds were found of which seven have been identified in pepper fruit extracts for the first time. According to the results, whole pepper (WP) samples presented higher content in phenolic acids; meanwhile, the edible portion (EP) was higher in flavonoids. No differences were found in the antioxidant activity except for the FRAP assay where the WP sample showed higher radical scavenging activity. EP samples showed the highest content of carotenoids and WP ones in chlorophylls