Immunocytochemical Study Of Cb1 Receptors In Rat ‘s Periaqueductal Gray After Cold Stress And Effects Of Peptides Tyr -W-Mif -1 And Tyr -K-Mif -1

The immunohistochemical localization of cannabinoid type 1 (CB1) receptors in periaqueductal gray (PAG) of male rats after acute cold stress and effects of endogenous antiopiate peptides Tyr-W-MIF-1 and Tyr-KMIF-1 on nociception was studied. The nociception was measured by the paw pressure test. As control were used intact rats. Stress activates PAG as an important component of the descending inhibitory pain pathway and stress-induced analgesia. CB1 immunoreactivity appeared as puncta and was found in cell bodies, axons and dendrites. The morphometric analysis revealed that acute cold stress increases the density of CB1-immunoreactive neurons in PAG compared with expression in intact animals. Secund, the results showed that Tyr-K-MIF-1 and Tyr-W-MIF-1 decreased the density of CB1-immunoreactive neurons in PAG of control rats, acute cold stress and after acute cold stress and effects of endogenous antiopiate peptides Tyr-WMIF-1 and Tyr-K-MIF-on nociception

BULGARIAN TRADITIONAL FOODS – SOURCES OF ANTIOXIDANTS

Many scientific studies are focused on antioxidant activities in prevention, on their beneficial role for health status and age retardation. The main source of antioxidants for humans is the diet. The aim of the study was to present data for antioxidant compounds in traditional Bulgarian foods, typical of Bulgarian diet. The traditional foods selected for this study were: onion, pepper, tomatoes, green beans, apples, and wild fruit varieties – blueberries, blackberries, raspberries. Antioxidant vitamins E and C were determined by RP-HPLC/fluorescence and UV detection. Total phenolics were determined by the Folin–Ciocalteu assay. Total flavonoids were measured by the aluminum chloride assay. Total carotenoids were determined by RP-HPLC/UV-Vis. The results showed that the highest amount of vitamin E was found in red peppers (2.94 mg/100g), and in raspberries (1.47 mg/100g), comparable with the level in sesame (2.4 mg/100g). Red peppers were evidenced to be the richest source of vitamin C (102 mg/100g). Total phenolics and total flavonoids as a marker of food antioxidant potential had the highest level in blueberries (670 mgGAE/100g, 190.3 mgCE/100g) and blackberries (355mgGAE/100g, 55.5 mgCE/100g). Total carotenoids are mostly detected in tomatoes (7.70 mg/100g), followed by spring onion (3.50 mg/100g). The modern analytical techniques enable the assessment of antioxidant constituents in some foods typical of the Bulgarian diet, and furthermore, on the basis of the results the assumption could be made that the Bulgarian longevity is associated not only with yogurt consumption, but with the abundance of antioxidants in the traditional diet as well