VALIDATION OF THE SPECTROFOTOMETRIC METHOD FOR THE DETERMINATION OF QUANTITATIVE COMPOSITION OF S-2,6-DIAGENOXIC ACID OF 3-METHYL-1,2,4-TRIAZOLYL- 5-THIOACETATE

Objective: The aim of the work was to determine the validation characteristics for the designed procedure for quantitation of the (S)-2,6- diaminohexanoic acid substance, 3-methyl-1,2,4-triazolyl-5-thioacetate, by absorption spectrophotometry in the ultraviolet region.Methods: For study purposes, the substance (S)-2,6-diaminohexanoic acid of 3-methyl-1,2,4-triazolyl-5-thioacetate and a standard sample (SS) obtained from the State Enterprise Chemical Reagents Plant†of the Scientific-Technological Complex Institute of Single Crystals†of the National Academy of Sciences of Ukraine, was used. In the course of our work, we used chemical, physical, physicochemical (absorption spectrophotometry in the ultraviolet region), and statistical methods of analysis. The study was carried out at the Laboratory for Standardization and Technology of Medicines at the Department of Pharmaceutical Chemistry at the Zaporizhia State Medical University (Ukraine).Results: In previous studies, we have developed a technique for spectrophotometric determination of the quantitative composition of (S)-2,6- diaminohexanoic acid of 3-methyl-1,2,4-triazolyl-5-thioacetate. The results of the conducted studies showed that the absorption curve in the UV region of the substance solution has a maximum absorption, namely: λ = 238 nm. The results of the quantitation of the substance (S)-2,6-diaminohexanoic acid of 3-methyl-1,2,4-triazolyl-5-thioacetate prove that our technique is accurate and reproducible and meets the current requirements. In order for the developed methodology to ensure consistent and accurate analysis results, its validation was carried out. According to the requirements of the State Pharmacopoeia of Ukraine (DFU) (2 editions), the developed method for the quantitation of the substance was checked for such validation characteristics as specificity, linearity, range of use, accuracy, correctness, and robustness.Conclusion: As a result of the work, it was proved that the developed method of the quantitation of the substance (S)-2,6-diaminohexanoic acid of 3-methyl-1,2,4-triazolyl-5-thioacetate by spectrophotometric method is valid

METABOLITHOTROPIC ASPECTS OF CARDIOPROTECTIVE ACTION OF NEW COMBINED MEDICINE BASED ON L-ARGININE AND THIOTRIAZOLIN AT MODELING OF MYOCARDIAL INFARCTION

  Objectives: The actual problem of modern medicine is the development of medications in treatment of cardiovascular system diseases. Different combinations of L-arginine with thiotriazolin in acute myocardial ischemia were examined as part of study and the optimal combination has been established - 4:1, named Argitryl.†The aim is to study the antioxidant, energotropic, and nitric oxide (NO)-modulating mechanisms of the cardioprotective effects of L-arginine with thiotriazolin (4:1) (Argitryl) drug candidate compared to 4.2% solution for injections Tivortin†(TOV Yuriya-Farm,†Ukraine) on the model of pituitrin-isadrin infarction of myocardium in laboratory rats.Methods: The study was conducted in accordance with Guidelines of State Expert Center of the Ministry of Health of Ukraine. All manipulations were carried out in accordance with the European Convention for the protection of vertebrate animals used for experimental and other scientific purposes.†The experiments were performed on 40 white outbred rats weighing 170–180 g. The antioxidant activity of the drugs was assessed by reduction of biochemical marker oxidative stress and an increase in the activity of the antioxidant system of the myocardium.Results: The study of energy metabolism has shown that the modeling of myocardial infarction leads to typical ischemic injuries - decrease in ATP, discoordination of the Krebs cycle, activation of anaerobic glycolysis in the cytosolic fraction of the heart of rats from the control group. Administration of Tivortin to animals does not lead to a significant decrease in ischemic injuries in the energy metabolism of the myocardium. The brightest anti-ischemic effect was observed with the appointment of Argitryl.â€Conclusion: The ascertained braking reaction of oxidative stress compared to the control group was found as a result of administration of Argitryl and Tivortin to animals with pituitrin-isadrin myocardial infarction. It was also found that the administration of Argitryl and Tivortin has a positive effect on the indices of the energy metabolism of the ischemic myocardium and leads to the normalization in conjugated NO system/renewed thiols. Argitryl significantly exceeds Tivortin on the influence of antioxidant, energy-tropic, and NO-modulating mechanisms of cardioprotective and anti-ischemic action on the studied indicatorsÂ

VALIDATION OF THE QUANTITATION METHODS OF 1-(β-PHENYLETHYL)-4-AMINO-1,2,4- TRIAZOLE BROMIDE SUBSTANCE BY SPECTROPHOTOMETRIC METHOD

 Objective: The aim of the work was to determine the validation characteristics for the developed method of quantitation of 1-(β-phenylethyl)-4- amino-1,2,4-triazole bromide substance by absorption spectrophotometry in the ultraviolet region (UV).Methods: For study purposes, the 1-(β-phenylethyl)-4-amino-1,2,4-triazole bromide substance and the standard sample, obtained by the state enterprise plant of chemical reagents†of scientific and technological complex institute of single crystals†of the National Academy of Sciences of Ukraine, was used. In the course of our work, we used chemical, physical, physicochemical (absorption spectrophotometry in the UV region), and statistical methods of analysis. The study was carried out at the Laboratory for Standardization and Technology of Medicines at the Department of Pharmaceutical Chemistry at Zaporizhzhia State Medical University (Ukraine).Results: In the previous studies, we have developed a technique for spectrophotometric determination of the quantitative content of 1-(β-phenylethyl)- 4-amino-1,2,4-triazole bromide substance. The results of the studies showed that the absorption curve in the UV region of the substance solution has three absorption maxima, namely: λ1=252 nm, λ2=258 nm, and λ3=263 nm. To quantify the substance, the wavelength λ2=258 nm was used. The results of the quantitation of the 1-(β-phenylethyl)-4-amino-1,2,4-triazole bromide substance by spectrophotometric method prove that the technique developed by us is accurate and reproducible and meets the current requirements. To the developed methodology to ensure accurate and accurate analysis results, its validation was carried out. According to the requirements of the State Pharmacopoeia of Ukraine (2 editions), the developed method for quantitation of the substance was checked for such validation characteristics as specificity, linearity, range of application, accuracy, correctness, and robustness.Conclusions: As a result of the work, it was proved that the method of quantitation of 1-(β-phenylethyl)-4-amino-1,2,4-triazolе bromide substance by spectrophotometric method is valid