Bar plots describing metabolite variations in the study population stratified in four quartiles according to visceral fat adiposity (intraperitoneal fat) at V2.

<p>Statistical significance is reported in Table S3. Key: PC-O, 1-O-alkyl-2- acylglycerophosphocholines. Assignment of PC-O species is made on the assumption that only even numbered carbon chains are present. A potential overlap between PC species containing odd-chain fatty acids and even-chained PC-O species cannot be excluded with low mass resolution.</p

Statistically significant Spearman correlation map between body fat composition parameters and clinical measures (95% confidence interval).

<p>Log₁₀ values of IPVF, VAT/SAT, VAT/total abdominal fat were strongly associated with HOMA-IR (r = 0.39, p = 0.015; r = 0.56, p<0.001; r = 0.55, p<0.001) and fasting insulin (r = 0.35, p = 0.0275; r = 0.49, p = 0.0017; r = 0.48, p = 0.0020). Strong associations were observed with ALAT (r = 0.39, p = 0.0128; r = 0.37, p = 0.0175; r = 0.38, p = 0.0167) and ALAT/ASAT ratio (r = 0.44, p = 0.0044; r = 0.35, p = 0.0268; r = 0.35, p = 0.0302). IPVF and Log₁₀ values of IPVF correlated with waist (r = 0.55, p<0.001; r = 0.35, p = 0.04) and waist/hip ratio (r = 0.69, p<0.001; r = 0.52, p = 0.0017), but not Log₁₀ values of VAT/SAT and VAT/total abdominal fat. NB: Blue denotes negative correlation, orange denotes positive correlation, and black denotes no correlation.</p

Plot describing metabolite importance and robustness in predicting visceral fat adiposity as assessed by Random forest analysis using metabolic data collected at V0 and V2.

<p>Visceral adiposity was associated with increasing concentrations of amino acids (glutamine, leucine/isoleucine, phenylalanine and tyrosine), lysophosphatidylcholine LPC 24∶0 and diacyl phospholipids (PC 30∶0, PC 34∶4). In addition, visceral adiposity was marked by a depletion in ether lipid species PC<i>-O</i> 36∶3, PC<i>-O</i> 40∶3, PC<i>-O</i> 40∶4, PC<i>-O</i> 40∶6, PC<i>-O</i> 42∶2, PC<i>-O</i> 42∶3, PC<i>-O</i> 42∶4, PC<i>-O</i> 44∶3, PC<i>-O</i> 44∶4, PC<i>-O</i> 44∶6, and two diacyl phosphocholines (PC 42∶0 and PC 42∶2). To reflect the weight of the selected biomarkers in the classification of visceral adiposity, a pooled mean decrease of accuracy for each compound was calculated from 10000 forest generations. Higher variable importance corresponds to higher values of pooled mean decrease in accuracy. Key: IPVF, intraperitoneal fat volume; LPC, Lysophosphatidylcholines; PC, Phosphatidylcholines; PC-O, 1-O-alkyl-2- acylglycerophosphocholines; Ratio1, intraperitoneal/subcutaneous fat ratio; Ratio 2, intraperitoneal/abdominal fat ratio. Assignment of PC-O species is made on the assumption that only even numbered carbon chains are present. A potential overlap between PC species containing odd-chain fatty acids and even-chained PC-O species cannot be excluded with low mass resolution.</p

Metabolite variations across subjects stratified according to intraperitoneal/abdominal fat ratio.

<p>NB: Blood plasma metabolites highlighted by multivariate analyses are reported as mean values ± SD. Key: Qi: data for population quartile i according to intraperitoneal/abdominal fat ratio. 12-HETE, 12-hydroxy-eicosatetraenoic acid; 15-HETE, 12-hydroxy-eicosatetraenoic acid; 9-HODE, 9-Hydroxy-10,12-octadecadienoic acid; AA, arachidonic acid; LPC, Lysophosphatidylcholines; PC, Phosphatidylcholines; PC-O, 1-O-alkyl-2- acylglycerophosphocholines; SM, Sphingomyelines; SM-OH, Hydroxy-Sphingomyelin.</p>*<p>Assignment of PC-O species is made on the assumption that only even numbered carbon chains are present. A potential overlap between PC species containing odd-chain fatty acids and even-chained PC-O species cannot be excluded with low mass resolution.</p

Descriptive statistics of subjects stratified according to intraperitoneal/abdominal fat ratio.

<p>Key: Qi: data for population quartile i according to intraperitoneal/abdominal fat ratio. BMI = body mass index, HDL-C =  high density lipoprotein cholesterol, homeostasis model assessment of insulin resistance =  HOMA-IR, LDL-C =  low density lipoprotein cholesterol, TG =  triglycerides, MAP =  mean arterial blood pressure, ALAT =  alanine aminotransferase, ASAT =  aspartate aminotransferase, GGT =  gamma-glutamyl transpeptidase, NEFAs = non esterified fatty acids. Data are reported as mean values ± SD.</p

Insulin and glucose response to oral glucose tolerance test according to intraperitoneal/abdominal fat ratio.

<p>NB: Values are reported as mean values ± SD. Key: Qi: data for population quartile i according to intraperitoneal/abdominal fat ratio.</p