Validation of the use of firearms for euthanising stranded cetaceans

Efforts to euthanise stranded cetaceans remain highly variable in their outcomes, with few field tested operational procedures available. This study sought to validate the efficacy of using modern firearms technology to euthanise small (<6m length) stranded cetaceans. Post-mortem evidence was gathered from the standardised shooting of cetacean cadavers (n = 10), representing six species, using .30 caliber (7.62mm) firearms and blunt solid copper-alloy non-deforming projectiles, in southwestern Australia. The six species studied were Risso's dolphin, common dolphin, bottlenosed dolphin, pygmy sperm whale, Cuvier's beaked whale, and humpback whale. Post-mortem data revealed that 100% of bullet wound tracts fully penetrated the skulls of shot animals, with associated indirect skull fracturing, secondary bone missiles and brain parenchyma laceration. The results suggest that appropriate firearms technology is fully capable of inducing instantaneous fatal pathology to the central nervous system of these species. In comparison to alternative methods for the euthanasia of stranded cetaceans, the use of firearms is associated with superior animal welfare outcomes, public safety levels and accessibility. This paper provides a template for the safe, humane and repeatable use of this technique to euthanise <6m length stranded cetaceans

Presentation and prognostic indicators for free-living black cockatoos (Calyptorhynchus SPP.) admitted to an Australian Zoo Veterinary Hospital over 10 years

The veterinary records of three species of free-living, endangered black cockatoos (Calyptorhynchus spp.; n5565) admitted to the Perth Zoo Veterinary Hospital in Western Australia during a 10-yr period (2000–09) were analyzed to determine the effect of clinical presentation and treatment on survival to release. The most-common reason for admission was trauma (at least 76.7%of cases), and trauma was also the most-frequent finding on necropsy examination (80.1% of cases). Anemia and paralysis-paresis were significant factors determining the decreased likelihood of survival of cockatoos undergoing rehabilitation. Human activities, in particular vehicle strike, were significant causes of morbidity and mortality in free-living black cockatoo populations

Standard metabolic rates of three nectarivorous meliphagid passerine birds

Standard metabolic rate (VO2 STD) was determined for three species of passerine bird from the family Meliphagidae to investigate the possible effect of nectarivory on standard metabolic rate in this family. The three species that we investigated did not show a significant departure from allometric predictions of standard metabolic rate for passerine species. Disparities between standard metabolic rate for meliphagids in the present study and previous data appear to reflect methodological differences, and no general allometric relationship is apparent for meliphagids at present. In meliphagids, nectarivory per se is not an important correlate with standard metabolic rate. Data from additional meliphagid species, collected under standardised conditions, are required to confirm the generality of the findings of the present study, that nectarivorous meliphagids have a standard metabolic rate typical of passerine birds

Cryptococcosis in Gilbert's and long-nosed potoroo

Two cases of fatal cryptococcosis are described, one of Cryptococcus neoformans infection in a Gilbert's potoroo (Potorous gilbertii) and one of Cryptococcus gattii infection in a long-nosed potoroo (Potorous tridactylus). The diagnoses were confirmed by culture and specific immunohistochemistry, respectively. The long-nosed potoroo tested positive using the latex cryptococcal antigen test (LCAT), whereas the Gilbert's potoroo had a negative LCAT result despite having advanced disease of some duration. In both cases, the clinical presentation was a progressive neurologic disease associated with a central nervous system infection. Pulmonary infection was also observed in the long-nosed potoroo. Specific treatment with antifungal agents was unsuccessful in the long-nosed potoroo

Development of reference ranges for plasma total cholinesterase and brain acetylcholinesterase activity in free ranging Carnaby's black cockatoos (Calyptorhynchus latirostris)

Published avian reference ranges for plasma cholinesterase (ChE) and brain acetylcholinesterase (AChE) are numerous. However, a consistently reported recommendation is the need for species- and laboratory-specific reference ranges because of variables, including assay methods, sample storage conditions, season, and bird sex, age, and physiologic status. We developed normal reference ranges for brain AChE and plasma total ChE (tChE) activity for Carnaby's Black-Cockatoos (Calyptorhynchus latirostris) using a standardized protocol (substrate acetylthiocholine at 25 C). We report reference ranges for brain AChE (19-41 μmol/min per g, mean 21±6.38) and plasma tChE (0.41-0.53 μmol/min per mL, mean 0.47±0.11) (n=15). This information will be of use in the ongoing field investigation of a paresis-paralysis syndrome in the endangered Carnaby's Black-Cockatoos, suspected to be associated with exposure to anticholinesterase compounds and add to the paucity of reference ranges for plasma tChE and brain AChE in Australian psittacine birds

A splendid tree frog with edema syndrome and intestinal adenocarcinoma

A splendid tree frog (Pelodryas splendida) presented with subcutaneous edema extending along its dorsum from head to vent, which resolved with improvement of ambient temperature and humidity conditions in its enclosure. Four months later, this same frog presented in a moribund state with intracoelomic fluid accumulation. An intestinal adenocarcinoma, a rarely reported neoplasm in amphibians, was diagnosed post mortem. Neoplasia is one of a number of causes of edema syndrome, which is a nonspecific response to disease and debility in anurans

An outbreak of mycobacteriosis in Gouldian finches caused by Mycobacterium peregrinum

An outbreak of mycobacteriosis was detected in an aviary containing Gouldian finches (Erythrura gouldiae) and golden shouldered parrots (Psephotus chrysopterygius). Affected birds developed granulomatous lesions, usually of the liver and intestine. Mycobacterium peregrinum, a species of the Mycobacterium fortuitum group, was cultured on pooled samples of intestinal tract from 31 euthanized finches. These rapid-growing mycobacteria are saprophytic organisms that are generally not associated with clinical disease in immunocomponenet hosts. This is the first report of mycobacteriosis in finches implicating M peregrinum as a causative agent

Hematologic and plasma biochemical reference values for three species of black cockatoos (Calyptorhynchus species)

Black cockatoos in southwest Western Australia face population declines as a result of habitat loss and fragmentation, poaching, competition with other species, vehicle strikes, and shootings. In this study, hematologic and plasma biochemical reference values were determined for 3 endemic Western Australian black cockatoo species: Carnaby's cockatoo (Calyptorhynchus latirostris) (n = 34), Baudin's cockatoo (Calyptorhynchus baudinii) (n = 22), and forest red-tailed black cockatoo (Calyptorhynchus banksii naso) (n = 22). Significant sex-related differences were detected in Carnaby's cockatoos, and differences were also found among the 3 species. The forest red-tailed black cockatoos showed a markedly greater eosinophil count than did the 2 white-tailed species. Blood values from samples obtained by different collection methods differed significantly for Carnaby's cockatoos, which indicates that anesthesia may affect analyte values and that collection methods should remain consistent. The results of this study will be useful for clinically evaluating wild black cockatoos that are undergoing rehabilitation for release and will provide baseline data for future investigations of free-living black cockatoo health

Reptarenaviruses in apparently healthy snakes in an Australian zoological collection

Background Inclusion body disease (IBD) is a disease of snakes with a global distribution and has recently been shown to be caused by reptarenaviruses. Testing for this group of viruses in asymptomatic snakes allows the association between infection and disease to be further elucidated. Methods A reptarenavirus was detected by RT‐PCR in a reticulated python (Malayopython reticulatus) from an Australian zoological collection that was open‐mouth breathing and had erythematous oral mucosa. Another 27 pythons, 4 elapids, 2 colubrids and 2 boas from this collection were then screened. From these animals, swabs, whole blood and/or tissue were tested for reptarenaviruses by RT‐PCR. Additionally, blood films from 10 snakes were examined by light microscopy for the presence of inclusion bodies. The majority of samples were collected over a 484‐day period. Results A total of 8 animals were RT‐PCR‐positive (8/36 = 22.2%): 6 were pythons, 1 was a corn snake (Pantherophis guttatus) and 1 was a Madagascar tree boa (Sanzinia madagascariensis). From them, 57 samples were collected, but only one from each animal was RT‐PCR‐positive (8/57 = 14.0%). From all 36 animals in this study, 8/182 samples were RT‐PCR‐positive (4.4%). Inclusion bodies were not recognised in any of the blood films. Only the reticulated python showed signs of illness, which improved without any further intervention. All other RT‐PCR‐positive snakes were apparently healthy throughout the duration of the study. Conclusion This study showed a weak association between the presence of reptarenaviruses and disease. Testing serially collected swab and whole‐blood samples increased the number of animals in which reptarenaviruses were detected