New insight into the Pleistocene deposits of Monte delle Piche, Rome, and remarks on the biochronology of Hippopotamus (Mammalia, Hippopotamidae) and Stephanorhinus etruscus (Mammalia, Rhinocerotidae) in Italy

Several large mammal assemblages have been collected in the Roman basin since the XIX century, but they usually lack any stratigraphic datum or details about the fossiliferous localities. In this work, the stratigraphic provenance of large mammal remains discovered at Monte delle Piche (Rome) is investigated. The systematic revision of these specimens allows the recording of the presence of Hippopotamus sp., Stephanorhinus sp. and Stephanorhinus etruscus. On the basis of micropalaeontological analysis performed on sediment sampled from the studied specimens and considering the stratigraphy of the area, two fossiliferous levels are recognised at Monte delle Piche. The remain of the hippopotamus was collected in fluvial gravels and sand deposits, in which the presence of Cyprideis is also recorded. This deposit is chronologically related to the latest Early-early Middle Pleistocene. Hippopotamus was present in Italy and Western Europe from the latest Villafranchian to MIS 4/3. The mandible of S. etruscus was collected in marine deposits along with abundant foraminifera and ostracods, which correlate with the late Early Pleistocene. Stephanorhinus etruscus occurred in Western Europe at the beginning of the Villafranchian, and it was documented until the end of the Villafranchian in Italy and until the Early-Middle Pleistocene transition in the Iberian Peninsula

Cytosol Mg2+ modulates Ca2+ ionophore induced secretion from rabbit neutrophils

AbstractThe influence of extracellular Mg2+ on Ca2+ ionophore (A23187 and ionomycin) induced secretion and changes in the cytosol pH of rabbit neutrophils suspended in Ca2+-free buffer has been investigated. While extracellular Ca2+ is obligatory for ionomycin induced secretion, we have defined conditions under which A23187 can induce secretion in Ca2+-free media. The different behaviour of these two Ca2+ ionophores is discussed on the basis of their different counter cation specificities

A 1.82 m^2 ring laser gyroscope for nano-rotational motion sensing

We present a fully active-controlled He-Ne ring laser gyroscope, operating in square cavity 1.35 m in side. The apparatus is designed to provide a very low mechanical and thermal drift of the ring cavity geometry and is conceived to be operative in two different orientations of the laser plane, in order to detect rotations around the vertical or the horizontal direction. Since June 2010 the system is active inside the Virgo interferometer central area with the aim of performing high sensitivity measurements of environmental rotational noise. So far, continuous not attempted operation of the gyroscope has been longer than 30 days. The main characteristics of the laser, the active remote-controlled stabilization systems and the data acquisition techniques are presented. An off-line data processing, supported by a simple model of the sensor, is shown to improve the effective long term stability. A rotational sensitivity at the level of ten nanoradiants per squareroot of Hz below 1 Hz, very close to the required specification for the improvement of the Virgo suspension control system, is demonstrated for the configuration where the laser plane is horizontal

Rotational sensitivity of the "G-Pisa" gyrolaser

G-Pisa is an experiment investigating the possibility to operate a high sensitivity laser gyroscope with area less than $1 \rm m^2$ for improving the performances of the mirrors suspensions of the gravitational wave antenna Virgo. The experimental set-up consists in a He-Ne ring laser with a 4 mirrors square cavity. The laser is pumped by an RF discharge where the RF oscillator includes the laser plasma in order to reach a better stability. The contrast of the Sagnac fringes is typically above 50% and a stable regime has been reached with the laser operating both single mode or multimode. The effect of hydrogen contamination on the laser was also checked. A low-frequency sensitivity, below $1 \rm Hz$, in the range of $10^{-8} \rm {(rad / s)/ \sqrt{Hz}}$ has been measured.Comment: 6 pages, 6 figures, presented at the EFTF-IFCS joint conference 200

Chelation of cytoplasmic Ca2+ increases plasma membrane permeability in murine macrophages.

Cytoplasmic free Ca2+ (Ca2+i) was chelated to 10-20 nM in the macrophage cell line J774 either by incubation with quin2 acetoxymethyl ester in the absence of external Ca2+ (Di Virgilio, F., Lew, P.D., and Pozzan, T. (1984) Nature 310, 691-693) or by loading [ethyl-enebis(oxyethylenenitrilo)]tetraacetic acid (EGTA) into the cytoplasm via reversible permeabilization of the plasma membrane with extracellular ATP (Steinberg, T.H., Newman, A.S., Swanson, J.A., and Silverstein, SS.C. (1987) J. Biol. Chem. 262, 8884-8888; Di Virgilio, F., Meyer, B.C., Greenberg, S., and Silverstein, S.C. (1988) J. Cell Biol. 106, 657-666). After removal of ATP from the incubation medium, ATP-permeabilized Ca2+i-depleted macrophages recovered a near-normal plasma membrane potential which slowly depolarized over a 2-4 h incubation at low [Ca2+]i. In both ATP-treated and quin2-loaded cells, depolarization of plasma membrane potential was paralleled by an increase in plasma membrane permeability to low molecular weight aqueous solutes such as eosin yellowish (Mr 692), ethidium bromide (Mr 394), and lucifer yellow (Mr 463). This increased plasma membrane permeability was not accompanied by release of the cytoplasmic marker lactic dehydrogenase for incubations up to 4 h and was likely a specific effect of Ca2+i depletion since it was not caused by: (i) the mere incubation of macrophages with extracellular EGTA, i.e. at near-normal [Ca2+]i; and (ii) loading into the cytoplasm of diethylenetriaminepentaacetic acid, a specific chelator of heavy metals with low affinity for Ca2+. Treatment of Ca2+i-depleted cells with direct (phorbol 12-myristate 13-acetate) or indirect (platelet-activating factor) activators of protein kinase C prevented the increase in plasma membrane permeability. Down-regulation of protein kinase C rendered Ca2+i-depleted macrophages refractory to the protective effect of phorbol 12-myristate 13-acetate. This report suggests a role for Ca2+i and possibly protein kinase C in the regulation of plasma membrane permeability to low molecular weight aqueous solutes

A dynamic rating curve approach to indirect discharge measurement

Abstract. The operational measurement of discharge in medium and large rivers is mostly based on indirect approaches by converting water stages into discharge on the basis of steady-flow rating curves. Unfortunately, under unsteady flow conditions, this approach does not guarantee accurate estimation of the discharge due, on the one hand, to the underlying steady state assumptions and, on the other hand, to the required extrapolation of the rating curve beyond the range of actual measurements used for its derivation. Historically, several formulae were proposed to correct the steady-state discharge value and to approximate the unsteady-flow stage-discharge relationship. In the majority of these methods, the correction is made on the basis of water level measurements taken at a single cross section where a steady state rating curve is available, while other methods explicitly account for the water surface slope using stage measurements in two reference sections. However, most of the formulae available in literature are either over-simplified or based on approximations that prevent their generalisation. Moreover they have been rarely tested on cases where their use becomes essential, namely under unsteady-flow conditions characterised by wide loop rating curves. In the present work, an original approach, based on simultaneous stage measurements at two adjacent cross sections, is introduced and compared to the approaches described in the literature. The most relevant feature is that the proposed procedure allows for the application of the full dynamic flow equations without restrictive hypotheses. The comparison has been carried out on channels with constant or spatially variable geometry under a wide range of flood wave and river bed slope conditions. The results clearly show the improvement in the discharge estimation and the reduction of estimation errors obtainable using the proposed approach

Spin-dependent direct gap emission in tensile-strained Ge films on Si substrates

The circular polarization of direct gap emission of Ge is studied in optically-excited tensile-strained Ge-on-Si heterostructures as a function of doping and temperature. Owing to the spin-dependent optical selection rules, the radiative recombinations involving strain-split light (cG-LH) and heavy hole (cG-HH) bands are unambiguously resolved. The fundamental cG-LH transition is found to have a low temperature circular polarization degree of about 85% despite an off-resonance excitation of more than 300 meV. By photoluminescence (PL) measurements and tight binding calculations we show that this exceptionally high value is due to the peculiar energy dependence of the optically-induced electron spin population. Finally, our observation of the direct gap doublet clarifies that the light hole contribution, previously considered to be negligible, can dominate the room temperature PL even at low tensile strain values of about 0.2%