Metabolic Epidermal Necrosis in Dog: a Case Report

Metabolic epidermal necrosis (MEN) was diagnosed in an unspayed nine-year-old male Poodle, associated with interface hepatitis and with active chronic pancreatitis. Also, a tumour formation was noticed at the cranial pole of the right kidney, both in ultrasound and in laparotomy examination. The histopathology did not establish the type of tumour, due to a human error. It is the first case of MEN in dogs recorded in Romania

EPIDEMIOLOGIC, CLINIC AND ETHIOPATHOGENIC STUDIES IN CANINE UROLITHIASIS

In a study performed on 983 examined dogs, urolithiasis was diagnosed in 20 cases, representing 2,03%. The highest incidence of urolithiasis was registered at the pure breeds (80%), most of them represented by males (75%). In the order of their appearance, the major symptoms for which the owners requested veterinary consult, were: anuria or dysuria (55%), bloody urine (40%), depression (40%), polakysuria (30%), stranguria (25%), urinary incontinence (15%). Using the ultrasound technique, we identified sediments in the urinary bladder in 80% of the dogs, uroliths in 20% (3 cases) and nephroliths in one case. Most of the encountered crystals were struvites (80%), cystine (10%), calcium oxalate (5%) and calcium phosphate (5%). Urolithiasis and sediment formation was found secondary to: urinary infections in 40% of the patients, chronic renal diseases in 25%, hyperproteic diet (20%), inborn errors of metabolism (10%) and diabetes mellitus (5%)

PARACLINICAL ASPECTS OF HEMOSTASIS DISTURBS IN PURPURA HEMORRHAGICA OF THE HORSES

A number of 20 work horses, clinically healthy (group no.1, control group) and 9 work horses with purpura hemorrhagica (group no.2) were investigated paraclinically using the parameters of the minimum balance of homeostasis. In both groups we determined the prothrombine time (PT), partial activated thromboplastine time (APTT) and fibrinogen. Using the average value of PT in group no.1 and group no.2 we calculated the INR value (International Normalized Ratio). The values of PT, APTT and INR were increased in horses from the 2nd group (PT= 56,43 ±86,48s; APTT = 95,53 ±14,81s; INR = 5,03) compared to the average values of the same parameters in the horses from group no. 1 (PT = 11,19 ±1,76s; APTT = 58,17 ±11,72s; INR = 1,10). Plasmatic fibrinogen was of 183 ±49,46 mg/dl in the horses of the 1st group and 136 ±57,57 mg/dl in the horses of the 2nd group

SERUM CORTISOL VARIATION OF SPORT HORSES IN RELATION WITH THE LEVEL OF TRAINING AND EFFORT INTENSITY

The aim of this research was to determine the influence of physical effort on serum cortisol values at horses in relation with physic training level and competition experience. Having in view the two parameters and the equine sport types, the horses have been divided in four groups: - group I and III , horses with a mean physic-training and a reduced competition experience - group II and IV , horses with a very good physic training and competition experience The horses from the first and second groups competed in the cross phase of the complete riding test, and those from the third and fourth groups competed in the two-hand marathon test. The results obtained in this experiment showed that the serum cortisol values were significantly increased (

Seroprevalence of Neosporosis in Dogs from Center and North-West of Romania

Seroprevalence of Neosporosis in Dogs from Center and North-West of Romani

Development of an Indirect ELISA Test Using Tachyzoite Crude Antigen for Sero-Diagnosis of Sheep Toxoplasma gondii Infection

The aim of this study was to use crude T. gondii tachyzoite antigen in an indirect ELISA and to compare it with indirect fluorescent antibody test (IFAT) considered as a “gold standard”. Sheep serum samples with known (49 negative and 45 positive) and unknown (94) status were tested. It was tried two types of microtiter plates and different antigen, sera and bloking dilution. The sensitivity, specificity positive and negative predictive value, accuracy and efficiency of test were evaluated. The chosen dilutions after CBT were 1:1000 for antigen and 1:400 for controls/sera samples on PolySorp 96-well microtiter plate (Nunc). 3% BSA had the best results in bloking phase of assay. The ELISA agreement with IFAT was relatively very good (k=0.81). The sensitivity and specificity of ELISA were 95.75%, respectively 85.11%. The accuracy of test was 81% and the efficiency 91.35%. ELISA using a crude tachyzoite antigen can be used for detecting anti-T. gondii antibodies in sheep sera, and consequently in the epidemiological studies and its uses instead of a commercial kit reduce the cost of the analysis

Research on the Etiology of Skin Diseases in Laboratory Animals

The objective of this study was to establish dermatological diseases etiology in laboratory animals. Were examined clinically and dermatologically a number of 403 rodents of different ages and sexes, respectively 173 guinea pigs (Cavia porcellus), 120 rabbits (Orytolagus cuniculus), 80 rats (Rattus norvegicus) and 30 mice (Mus musculus). Dermatological diseases identified in guinea pigs were represented by malophagosis (Gliricola porcelli in 111/173 - 64,16%), listrophorosis (Chirodiscoides caviae in 111/173 - 64,16%), demodicosis (Demodex caviae in 2/173 - 1,15%) and tricophytosis (Trichophyton mentagrophytes in 3/173 – 1,73%). Rats were infected only with Notoedres muris (56/80 respectively 70%). In rabbits was diagnosed (Psoroptes cuniculi in 7/120 – 5,83%), listrophorosis (Listrophorus gibbus in 2/120 – 1,66%) and cheyletiellosis (Cheiletiella parasitovorax in 2/120 – 1,66% ). In mice have evolved associated infestations with Mycoptes musculinus (20/30 - 66,66%), Myobia musculi (12/30 - 40%) and Chirodiscoides caviae (3/30 - 10%). Chirodiscoides caviae, mite with specific parasitism in guinea pig, has been isolated from mice with skin lesions. Were recorded two zoonotic diseases: tricophytosis and cheyletiellosi

Seroprevalence of Toxoplasma gondii and Neospora caninum in Dairy Goats from Romania

Seroprevalence of Toxoplasma gondii and Neospora caninum in Dairy Goats from Romani

EPIDEMIOLOGY AND ETIOLOGY IN SHEEP SARCOCYSTOSIS

The aim of the present study was to investigate the prevalence, intensity of infection and etiology of sarcocystosis in sheep slaughtered in two abattoirs. The prevalence of intestinal sarcocystosis in naturally infected dogs and cats were also explored. Development of sporocysts in the small intestine of dogs and cats has also been investigated. The presence of macrocysts was observed in 10/38 carcasses being noticed only in esophagus. Concerning the prevalence of microcysts, the highest prevalence was observed in esophagus (81.6%), followed by myocardium (79.9%) and diaphragm (68.8%). In myocardium there was the highest number of mycrocysts (162 microcysts/g muscle tissue). The prevalence of intestinal sarcocystosis in naturally infected dogs and cats was 1.1%, respectively 1.8%. The pre-patent period in experimentally infected dogs was 12 days and the patent period lasted 60 days. At the microscopic exam (40X) there were noticed a mean of 4 sporocysts/microscopic field at 17 days post-inoculation. The size of sporocysts was 15.14±0.72/10.26±0.69 µm. No sporocysts were shed by cats. The microcysts were identify as Sarcocystis ovicanis (S. tenella)

Drug Resistance of Eimeria Field Isolates from Commercial Broiler Flocks in Romania: Preliminary Results

Drug Resistance of Eimeria Field Isolates from Commercial Broiler Flocks in Romania: Preliminary Result